Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (12): 2405-2410.DOI: 10.3969/j.issn.1000-2561.2019.12.013

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Transformation of Tissues of Litchi Fruit with Injection of Agrobacterium

WANG Shujun,WANG Guo,LI Huanling,SUN Jinhua,LI Fang,WANG Jiabao()   

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2019-05-20 Revised:2019-07-04 Online:2019-12-25 Published:2019-12-20
  • Contact: WANG Jiabao


To find an efficient transient gene expression method for litchi, the fruits of ‘Xinqiumili’ were used as the material to transform fruit tissues by Agrobacterium in this study. The effects of fruit developing stage, bacterium strains, injection sites, sampling time, bacteria solution concentration on the transformation efficiency were studied. The results showed that the GUS staining rates were better in four tissues under the condition that Agrobacterium strain GV3101 with OD600 of 2.4 was injected into the stalk, peel, seed and pulp of the stage Ⅱ b fruit with the pulp completely wrapped in the seed in vivo, and the fruits were sampled for detection after four days of injection. The transient gene expression system of litchi fruits was successfully established, which laid a foundation for the rapid identification of related genes of litchi fruits in the future.

Key words: injection of Agrobacterium, ‘Xinqiumili’ fruit, transient expression, GUS detection

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