Transformation of Tissues of Litchi Fruit with Injection of Agrobacterium

doi:10.3969/j.issn.1000-2561.2019.12.013

Abstract

Abstract:

To find an efficient transient gene expression method for litchi, the fruits of ‘Xinqiumili’ were used as the material to transform fruit tissues by Agrobacterium in this study. The effects of fruit developing stage, bacterium strains, injection sites, sampling time, bacteria solution concentration on the transformation efficiency were studied. The results showed that the GUS staining rates were better in four tissues under the condition that Agrobacterium strain GV3101 with OD₆₀₀ of 2.4 was injected into the stalk, peel, seed and pulp of the stage Ⅱ b fruit with the pulp completely wrapped in the seed in vivo, and the fruits were sampled for detection after four days of injection. The transient gene expression system of litchi fruits was successfully established, which laid a foundation for the rapid identification of related genes of litchi fruits in the future.

Key words: injection of Agrobacterium, ‘Xinqiumili’ fruit, transient expression, GUS detection

CLC Number:

S667.1

WANG Shujun,WANG Guo,LI Huanling,SUN Jinhua,LI Fang,WANG Jiabao. Transformation of Tissues of Litchi Fruit with Injection of Agrobacterium[J]. Chinese Journal of Tropical Crops, 2019, 40(12): 2405-2410.

Figures/Tables 7

Fig. 1 Effects of strains on fruit set percentage

Fig. 2 Effects of strains on GUS staining rate

Fig. 3 Morphology and anatomy of litchi fruit

Fig. 4 Color change accelerated after Agrobacterium injection in litchi peel

Fig. 5 Effects of sampling time on GUS staining rate Different letters indicate significant difference of GUS staining rates between different sampling times in the same tissues (P<0.05).

Fig. 6 Effects of bacteria solution concentration on GUS staining rate Different letters indicate significant difference of GUS staining rates between different sampling times in the same tissue (P<0.05).

Fig. 7 GUS staining results in different fruit tissues sampled after four days of Agrobacterium injection

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