Abstract:

Based on SLAF-seq technology, 122 sweet potato germplasm resources were used to develop SNP sites, which were applied to the analysis of population structure and genetic evolution on all germplasm resources. Results showed that 563.18 Mb reads length was obtained by sequencing, the reads of the samples varied from 1 419 809 to 8 392 785. The sequencing quality value (Q30) changed from 90.61% to 96.82%, and the average Q30 was 92.78%. The GC content of the samples changed from 36.46%-40.33%, the average value was 38.17%, and that of the control was 40.72%. A total of 2 388 759 SLAF tags were developed, with an average sequencing depth of 17.45×. There were 77 761 polymorphic SLAF tags accounting for 3.26% of the total SLAF tags. Finally, 129 063 SNPs were found. The population structure and genetic evolution analysis showed that 122 germplasm resources could be divided into three groups, which was not directly related to the geographical source, and the results of clustering could provide scientific basis for parents combination and utilization of heterosis in sweetpotato breeding.

Key words: sweetpotato, germplasm resources, SLAF-seq, phylogenetic analysis