Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (12): 2369-2374.DOI: 10.3969/j.issn.1000-2561.2019.12.009

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Physiological Responses and Evaluation of Cold Resistance under Cold Stress for Six Varieties of Eriobotrya japonica (Thunb.)

PAN Cuiping1,XIE Hongjiang2,WANG Yongqing1,*(),ZHANG Hui1,DENG Qunxian1,YANG Zhiwu1,WEN Lu1,HE Shanshan1   

  1. 1. College of Horticulture, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China
    2. Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China
  • Received:2019-05-28 Revised:2019-06-21 Online:2019-12-25 Published:2019-12-20
  • Contact: WANG Yongqing


In order to investigate the physiological response and comprehensively assess cold resistance of different varieties under low stress, six varieties of Eriobotrya japonica (Thunb.) under cold stress were analyzed to determine the dynamic changes of relative electric conductivity (REC), malonadehyde (MDA), free proline (Pro), soluble sugar and soluble protein. The results showed that the relative electric conductivity (REC) in vitro seed embryos of young fruits of six loquat varieties presented an ‘S’ type curve change pattern as temperature decreased. The semi-lethal temperature of the six loquat varieties ranging from -4.759 to -2.73 ℃ using logistic equation, the semi-lethal temperature of white bud mutation was the highest and the Dawuxing loquat was the lowest among them. The contents of free proline (Pro) and malonadehyde (MDA) presented an inverted “V” type curve change pattern with the different time point of reaching the peak, which generally increased first and then decreased under cold stress. Furthermore, the soluble sugar and soluble protein increased as temperature decreased with the maximum value was obtained at the time of 24 h when treated at -5 ℃. The cold resistance ability of the six loquat varieties evaluated as WT (white bud mutation) > Guanyu > Ninghaibai > Longquan No.5 > Zaozhong No.6 > Dawuxing. In conclusion, the WT (white bud mutation) showed strong adaptability to low temperature stress and strong cold resistance. The median-lethal temperature combined with comprehensive evaluation based on subordinate function analysis could accurately identify the cold resistance of loquat varieties, which would provide a theoretical foundation and reference for screening of cold materials and looking for a new approach to increase the cold hardiness of loquat.

Key words: loquat (Eriobotrya japonica (Thunb.) Lindl.), cold stress, physiological responses, cold resistance, evaluation

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