Physiological Responses and Evaluation of Cold Resistance under Cold Stress for Six Varieties of Eriobotrya japonica (Thunb.)

doi:10.3969/j.issn.1000-2561.2019.12.009

Abstract

Abstract:

In order to investigate the physiological response and comprehensively assess cold resistance of different varieties under low stress, six varieties of Eriobotrya japonica (Thunb.) under cold stress were analyzed to determine the dynamic changes of relative electric conductivity (REC), malonadehyde (MDA), free proline (Pro), soluble sugar and soluble protein. The results showed that the relative electric conductivity (REC) in vitro seed embryos of young fruits of six loquat varieties presented an ‘S’ type curve change pattern as temperature decreased. The semi-lethal temperature of the six loquat varieties ranging from -4.759 to -2.73 ℃ using logistic equation, the semi-lethal temperature of white bud mutation was the highest and the Dawuxing loquat was the lowest among them. The contents of free proline (Pro) and malonadehyde (MDA) presented an inverted “V” type curve change pattern with the different time point of reaching the peak, which generally increased first and then decreased under cold stress. Furthermore, the soluble sugar and soluble protein increased as temperature decreased with the maximum value was obtained at the time of 24 h when treated at -5 ℃. The cold resistance ability of the six loquat varieties evaluated as WT (white bud mutation) > Guanyu > Ninghaibai > Longquan No.5 > Zaozhong No.6 > Dawuxing. In conclusion, the WT (white bud mutation) showed strong adaptability to low temperature stress and strong cold resistance. The median-lethal temperature combined with comprehensive evaluation based on subordinate function analysis could accurately identify the cold resistance of loquat varieties, which would provide a theoretical foundation and reference for screening of cold materials and looking for a new approach to increase the cold hardiness of loquat.

Key words: loquat (Eriobotrya japonica (Thunb.) Lindl.), cold stress, physiological responses, cold resistance, evaluation

CLC Number:

S667.3

PAN Cuiping,XIE Hongjiang,WANG Yongqing,ZHANG Hui,DENG Qunxian,YANG Zhiwu,WEN Lu,HE Shanshan. Physiological Responses and Evaluation of Cold Resistance under Cold Stress for Six Varieties of Eriobotrya japonica (Thunb.)[J]. Chinese Journal of Tropical Crops, 2019, 40(12): 2369-2374.

Figures/Tables 8

Fig. 1 Dynamic changes of the relative electric conductivity in vitro seed embryos of young fruits of six loquat varieties under cold stress

Tab. 1 Logistic equation and the semi-lethal temperature of six loquat varieties

品种 Variety	Logistic方程 Logistic equation	R²	半致死温度LT₅₀ The median-lethal temperature/℃
WT	y=92.312/(1+8.214e^-0.431^x)	0.921	-4.759
冠玉	y=94.258/(1+9.325e^-0.461^x)	0.946	-4.02
宁海白	y=89.775/(1+7.841e^-0.495^x)	0.947	-3.81
龙泉5号	y=98.553/(1+6.964e^-0.478^x)	0.934	-2.98
早钟6号	y=96.742/(1+9.119e^-0.477^x)	0.911	-2.91
大五星	y=96.954/(1+6.665e^-0.492^x)	0.907	-2.73

Fig. 2 Dynamic changes of the contents of malonadehyde in vitro seed embryos of young fruits of six loquat varieties under cold stress

Fig. 3 Dynamic changes of the contents of free proline in vitro seed embryos of young fruits of six loquat varieties under cold stress

Fig. 4 Dynamic changes of the contents of soluble sugar in vitro seed embryos of young fruits of six loquat varieties under cold stress

Fig. 5 Dynamic changes of the contents of soluble protein in vitro seed embryos of young fruits of six loquat varieties under cold stress

Tab. 2 Comprehensive evaluation on cold resistance of six loquat varieties

指标 Indexes	WT White bud mutation	宁海白 Ninghaibai	冠玉 Guanyu	早钟6号 Zaozhong No.6	龙泉5号 Longquan No.5	大五星 Dawuxing
相对电导率	0.763	0.641	0.698	0.611	0.604	0.521
可溶性糖含量	0.819	0.608	0.671	0.366	0.413	0.354
可溶性蛋白含量	0.731	0.539	0.581	0.287	0.429	0.302
丙二醛含量	0.626	0.439	0.447	0.417	0.403	0.389
脯氨酸含量	0.857	0.555	0.564	0.488	0.523	0.471
平均隶属度	0.759	0.556	0.592	0.434	0.474	0.407
抗寒能力排序	1	3	2	5	4	6

Fig. 6 Cluster analysis on cold resistance of six loquat varieties

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