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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (12): 2356-2363.DOI: 10.3969/j.issn.1000-2561.2019.12.007

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Construction Strategy of Core Germplasm of Cherry Tomato

NIU Yu,LIU Weixia,YANG Yan,LIU Ziji()   

  1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China
  • Received:2019-05-29 Revised:2019-07-10 Online:2019-12-25 Published:2019-12-20
  • Contact: LIU Ziji

Abstract:

In order to effectively identify the core germplasm and promote the innovative utilization of cherry tomato germplasm, the genotype values of seven traits (leaf length, leaf width, first inflorescence node, fruit length, fruit width, flesh thickness and weight per fruit) of 219 cherry tomato germplasms were predicted by a mixed linear model. Cherry tomato core germplasms were constructed based on genotype values by adopting two genetic distance (Euclidean distance and Mahalanobis distance), eight clustering methods (Single linkage, Complete linkage, Centroid method, Median method, Unweighted pair-group average, Flexible-beta method, Weighted pair-group average and Ward’s method) and three sampling methods (Preferred sampling, Random sampling and Deviation sampling) with 30% sampling proportion. The core germplasms constructed by different methods were evaluated based on mean, variance, range and variation coefficient. The variance of seven traits of cherry tomato core germplasm constructed by Euclidean distance and Mahalanobis distance were higher than that of the original population. By Euclidean distance, the means of seven traits had no significant difference between the core germplasms and the original population. By Mahalanobis distance, except four traits, the means of the other three traits were significantly different between the core germplasms and the original population. The result indicated that Euclidean distance was better than Mahalanobis distance. The variance and variation coefficient of seven traits of the core germplasms constructed by eight clustering methods were higher than that of the original population. The cherry tomato core germplasm constructed by the single linkage method had relatively larger genetic variation, which was better than the other clustering methods. The variance and variation coefficient of seven traits of the core germplasms constructed by three sampling methods were higher than that of the original population. The deviation sampling was superior to the preferred sampling and random sampling method. A total of 65 cherry tomato core germplasms were selected based on Euclidean distance, single linkage clustering and deviation sampling, which could represent the genetic diversity of the original population.

Key words: cherry tomato, genotype value, genetic distance, clustering method, sampling method, core germplasm

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