Colletotrichum Species Responsible for Leaf Anthracnose of Liquidambar formosana Hance in Fujian

doi:10.3969/j.issn.1000-2561.2019.11.014

Abstract

Abstract:

Six morphologically different Colletotrichum strains were obtained during the investigation of Liquidambar formosana Hance leaf diseases in Fujian. The six strains were identified based on morphology (colony, acervuli, conidiophores and conidia) and multi-loci (ITS, TUB2, ACT, GAPDH and CHS-1) phylogenetic analyses. The six strains belonged to three Colletotrichum species, i.e., C. fructicola, C. tropicale and C. fioriniae, which belonged to Colletotrichum gloeosporioides complex and Colletotrichum actatum complex, respectively. The results from Koch’s postulates supported the three Colletotrichum species could cause leaf anthracnose to Liquidambar formosana, and the pathogenecity of them was different. This is the first report of the three Colletotrichum species to cause leaf anthracnose on Liquidambar formosana.

Key words: anthracnose, Liquidambar formosana, identification, phylogenetic analyses

ZHU Yangyan,SU Jinyu,PAN Aifang,HE Xueyou,HU Hongli. Colletotrichum Species Responsible for Leaf Anthracnose of Liquidambar formosana Hance in Fujian[J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2197-2204.

Figures/Tables 7

References 24

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物种 Species	菌株 Strain		基因登录号GenBank accession numbers
物种 Species	菌株 Strain		ITS		GAPDH			ACT			CHS1			TUB2
C. acutatum	CBS 112996		JQ005776		JQ948677			JQ005839			JQ005797			JQ005860
C. aenigma	ICMP 18686		JX010243		JX009913			JX009519			JX009789			JX010390
C. aeschynomenes	ICMP 17673		JX010176		JX009930			JX009483			JX009799			JX010392
C. alatae	CBS 304.67		JX010190		JX009990			JX009471			JX009837			JX010383
C. alienum	ICMP 12071		JX010251		JX010028			JX009572			JX009882			JX010411
C. aotearoa	ICMP 18537		JX010205		JX010005			JX009564			JX009853			JX010420
C. asianum	ICMP 18580		FJ972612		JX010053			JX009584			JX009867			JX010406
C. boninense	CBS 128526, ICMP 18591		JQ005162		JQ005249			JQ005510			JQ005336			JQ005596
C. chrysanthemi	CBS 126518		JQ948271		JQ948601			JQ949592			JQ948932			JQ949922
C. clidemiae	ICMP 18706		JX010274		JX009909			JX009476			JX009777			JX010439
C. cliviae	CBS 125375		JX519223		JX546611			JX519240			JX519232			JX519249
C. conoides	CAUG17		KP890168		KP890162			KP890144			KP890156			KP890174
C. cordylinicola	ICMP 18579		JX010226		JX009975			HM470235			JX009864			JX010440
C. dracaenophilum	CBS 118199		JX519222		JX546707			JX519238			JX519230			JX519247
C. endophytica	CAUG28		KP145441		KP145413			KP145329			KP145385			KP145469
C. fioriniae	CAUA18		KP145016		KP145096			KP145032			KP145048			KP145080
C. fructicola	CAUG1		KP145416		KP145388			KP145304			KP145360			KP145444
C. gloeosporiodes	CAUG2		KP145417		KP145389			KP145305			KP145361			KP145445
C. godetiae	CBS 133.44		JQ948402		JQ948733			JQ949723			JQ949063			JQ950053
C. grevillea	CBS 132879		KC297078		KC297010			KC296941			KC296987			KC297102
C. grossum	CAUG7		KP890165		KP890159			KP890141			KP890153			KP890171
C. guajavae	IMI 350839		JQ948270		JQ948600			JQ949591			JQ948931			JQ949921
C. hebeiense	JZB 330028		KF156863		KF377495			KF377532			KF289008			KF288975
C. hymenocallidis	CAUG9		KP145423		KP145395			KP145311			KP145367			KP145451
C. karstii	CAUOS8		KP890109		KP890140			KP890102			KP890133			KP890117
C. laticiphilum	CBS 112989		JQ948289		JQ948619			JQ949610			JQ948950			JQ949940
C. liaoningense	CAUOS2		KP890104		KP890135			KP890097			KP890127			KP890111
C. musae	ICMP 19119		JX010146		JX010050			JX009433			JX009896			HQ596280
C. nupharicola	ICMP 17938		JX010189		JX009936			JX009486			JX009834			JX010397
C. nymphaeae	CBS 515.78		JQ948197		JQ948527			JQ949518			JQ948858			JQ949848
C. orchidophilum	CBS 632.80		JQ948151		JQ948481			JQ949472			JQ948812			JQ949802
C. psidii	ICMP 19120		JX010219		JX009967			JX009515			JX009901			JX010443
C. queenslandicum	ICMP 1778		JX010276		JX009934			JX009447			JX009899			JX010414
C. salicis	CBS 607.94		JQ948460		JQ948791			JQ949781			JQ949121			JQ950111
C. salsolae	ICMP 19051		JX010242		JX009916			JX009562			JX009863			JX010403
C. scovillei	CAUA1		KP145208		KP145144			KP145112			KP145272			KP145240
C. siamense	CBS 130420		HM131511		HM131497			HM131507			JX009895			JX010415
物种 Species	菌株 Strain	基因登录号GenBank accession numbers
物种 Species	菌株 Strain	ITS		GAPDH			ACT		CHS1				TUB2
C. theobromicola	CBS 124945	JX010294		JX010006		JX009444				JX009869		JX010447
C. ti	ICMP 4832	JX010269		JX009952		JX009520				JX009898		JX010442
C. tropicale	CBS 124949	JX010264		JX010007		JX009489				JX009870		JX010407
C. viniferum	CAUG27	KP145440		KP145412		KP145328				KP145384		KP145468
C. xanthorrhoeae	ICMP 17903	JX010261		JX009927		JX009478				JX009823		JX010448
C. fructicola	FX HB 2	√		√		√				√		√
C. fructicola	FX HB 3	√		√		√				√		√
C. fructicola	FX HB SLGY 2	√		√		√				√		√
C. fructicola	FX YB 3	√		√		√				√		√
C. tropicale	FX OHB 1 2	√		√		√				√		√
C. fioriniae	FX YB SLGY 2	√		√		√				√		√

物种 Species	菌株 Strain		基因登录号GenBank accession numbers
物种 Species	菌株 Strain		ITS		GAPDH			ACT			CHS1			TUB2
C. acutatum	CBS 112996		JQ005776		JQ948677			JQ005839			JQ005797			JQ005860
C. aenigma	ICMP 18686		JX010243		JX009913			JX009519			JX009789			JX010390
C. aeschynomenes	ICMP 17673		JX010176		JX009930			JX009483			JX009799			JX010392
C. alatae	CBS 304.67		JX010190		JX009990			JX009471			JX009837			JX010383
C. alienum	ICMP 12071		JX010251		JX010028			JX009572			JX009882			JX010411
C. aotearoa	ICMP 18537		JX010205		JX010005			JX009564			JX009853			JX010420
C. asianum	ICMP 18580		FJ972612		JX010053			JX009584			JX009867			JX010406
C. boninense	CBS 128526, ICMP 18591		JQ005162		JQ005249			JQ005510			JQ005336			JQ005596
C. chrysanthemi	CBS 126518		JQ948271		JQ948601			JQ949592			JQ948932			JQ949922
C. clidemiae	ICMP 18706		JX010274		JX009909			JX009476			JX009777			JX010439
C. cliviae	CBS 125375		JX519223		JX546611			JX519240			JX519232			JX519249
C. conoides	CAUG17		KP890168		KP890162			KP890144			KP890156			KP890174
C. cordylinicola	ICMP 18579		JX010226		JX009975			HM470235			JX009864			JX010440
C. dracaenophilum	CBS 118199		JX519222		JX546707			JX519238			JX519230			JX519247
C. endophytica	CAUG28		KP145441		KP145413			KP145329			KP145385			KP145469
C. fioriniae	CAUA18		KP145016		KP145096			KP145032			KP145048			KP145080
C. fructicola	CAUG1		KP145416		KP145388			KP145304			KP145360			KP145444
C. gloeosporiodes	CAUG2		KP145417		KP145389			KP145305			KP145361			KP145445
C. godetiae	CBS 133.44		JQ948402		JQ948733			JQ949723			JQ949063			JQ950053
C. grevillea	CBS 132879		KC297078		KC297010			KC296941			KC296987			KC297102
C. grossum	CAUG7		KP890165		KP890159			KP890141			KP890153			KP890171
C. guajavae	IMI 350839		JQ948270		JQ948600			JQ949591			JQ948931			JQ949921
C. hebeiense	JZB 330028		KF156863		KF377495			KF377532			KF289008			KF288975
C. hymenocallidis	CAUG9		KP145423		KP145395			KP145311			KP145367			KP145451
C. karstii	CAUOS8		KP890109		KP890140			KP890102			KP890133			KP890117
C. laticiphilum	CBS 112989		JQ948289		JQ948619			JQ949610			JQ948950			JQ949940
C. liaoningense	CAUOS2		KP890104		KP890135			KP890097			KP890127			KP890111
C. musae	ICMP 19119		JX010146		JX010050			JX009433			JX009896			HQ596280
C. nupharicola	ICMP 17938		JX010189		JX009936			JX009486			JX009834			JX010397
C. nymphaeae	CBS 515.78		JQ948197		JQ948527			JQ949518			JQ948858			JQ949848
C. orchidophilum	CBS 632.80		JQ948151		JQ948481			JQ949472			JQ948812			JQ949802
C. psidii	ICMP 19120		JX010219		JX009967			JX009515			JX009901			JX010443
C. queenslandicum	ICMP 1778		JX010276		JX009934			JX009447			JX009899			JX010414
C. salicis	CBS 607.94		JQ948460		JQ948791			JQ949781			JQ949121			JQ950111
C. salsolae	ICMP 19051		JX010242		JX009916			JX009562			JX009863			JX010403
C. scovillei	CAUA1		KP145208		KP145144			KP145112			KP145272			KP145240
C. siamense	CBS 130420		HM131511		HM131497			HM131507			JX009895			JX010415
物种 Species	菌株 Strain	基因登录号GenBank accession numbers
物种 Species	菌株 Strain	ITS		GAPDH			ACT		CHS1				TUB2
C. theobromicola	CBS 124945	JX010294		JX010006		JX009444				JX009869		JX010447
C. ti	ICMP 4832	JX010269		JX009952		JX009520				JX009898		JX010442
C. tropicale	CBS 124949	JX010264		JX010007		JX009489				JX009870		JX010407
C. viniferum	CAUG27	KP145440		KP145412		KP145328				KP145384		KP145468
C. xanthorrhoeae	ICMP 17903	JX010261		JX009927		JX009478				JX009823		JX010448
C. fructicola	FX HB 2	√		√		√				√		√
C. fructicola	FX HB 3	√		√		√				√		√
C. fructicola	FX HB SLGY 2	√		√		√				√		√
C. fructicola	FX YB 3	√		√		√				√		√
C. tropicale	FX OHB 1 2	√		√		√				√		√
C. fioriniae	FX YB SLGY 2	√		√		√				√		√

菌株 Strains	叶片病斑长度 Lesion sizes on wounded leaves/cm	叶柄病斑长度 Lesion sizes on wounded petiole/cm
FX HB 2	0.94±0.11^a	0.47±0.16^a
FX HB SLGY 2	0.93±0.11^a	0.37±0.58^ab
FX YB 3	0.82±0.30^ab	0.12±0.16^bc
FX HB 3	0.74±0.11^bc	0.27±0.15^abc
FX OHB 1 2	0.90±0.18^a	0.48±0.19^a
FX YB SLGY 2	0.60±0.17^c	0.33±0.29^c

菌株 Strains	叶片病斑长度 Lesion sizes on wounded leaves/cm	叶柄病斑长度 Lesion sizes on wounded petiole/cm
FX HB 2	0.94±0.11^a	0.47±0.16^a
FX HB SLGY 2	0.93±0.11^a	0.37±0.58^ab
FX YB 3	0.82±0.30^ab	0.12±0.16^bc
FX HB 3	0.74±0.11^bc	0.27±0.15^abc
FX OHB 1 2	0.90±0.18^a	0.48±0.19^a
FX YB SLGY 2	0.60±0.17^c	0.33±0.29^c