Construction of Cassiicolin Genes Barcode Database and Molecular Detection Technology of <i>Corynespora cassiicola</i> from <i>Hevea brasiliensis</i> in China
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (9): 1770-1782.DOI: 10.3969/j.issn.1000-2561.2019.09.015

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Construction of Cassiicolin Genes Barcode Database and Molecular Detection Technology of Corynespora cassiicola from Hevea brasiliensis in China

LI Boxun,LIU Xianbao,FENG Yanli,XIE Huiting,HUANG Guixiu()   

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2019-03-01 Revised:2019-04-22 Online:2019-09-25 Published:2019-09-10
  • Contact: HUANG Guixiu

Abstract:

Corynespora cassiicola is a plant pathogenic fungus, which is harmful to major tropical crops in China. It has a wide range of hosts, significant morphological differences, diverse symptoms and contains a host-specific toxin, which encoding up to six distinct protein isoforms. In the study, we used public database to construct Cassiicolin genes barcode database of C. cassiicola form major tropical crops in China. At the same time, we established a set of molecular detection techniques with high specificity and sensitivity, which could detect 100 pg/μL target genomic DNA. Then, we systematically detected the isoforms cassiicolin of C. cassiicola, which were isolated from rubber tree, cassava, papaya, melon and vegetable in China. There were only two isoforms Cassiicolin in China, among which the Cas5 isoform Cassiicolin accounted for 94.8%, and had obvious host specificity and was specific and advantage population on rubber tree in China. Phylogenetic tree analysis showed that distinct isoforms Cassiicolin of C. cassiicola was closely related to host origin, but not to geographical origin. The results of the research would significantly contribute to the population genetic structure, dominant population, preservation of C. cassiicola and control strategies.

Key words: Corynespora cassiicola, Cassiicolin genes, DNA barcoding, molecular detection