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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1598-1605.DOI: 10.3969/j.issn.1000-2561.2019.08.021

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Isolation and Identification of Endophytic Fungi from Hainan Patchouli and Screening of Antagonistic Strains

YI Tianfeng,WU Yougen(),YU Jing,ZHANG Junfeng,YANG Dongmei   

  1. College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received:2018-12-20 Revised:2019-04-26 Online:2019-08-25 Published:2019-08-20
  • Contact: WU Yougen


In this study, 61 endophytic fungi were isolated from roots, stems, leaves and flowers of Hainan patchouli, and identified as 15 genera by ITS sequence analysis and morphological analysis. The dominant genus was the Colletotrichum and Meyerozyma. The inhibitory effect of Fusarium PfuJ20, PfuG16 and PfuG5 on Pestalotiopsis microspora was most obvious when Pestalotiopsis microspora, Fusarium oxysporum and Neoscytalidium dimidiatum were used as the indicator bacteria, and the activity of phytopathogenic fungi was determined by the plate sputum method. The activity of anti-phytopathogenic bacteria was determined by the filter paper diffusion method. Fusarium had strong inhibitory effects on the growth of Staphylococcus aureus, Escherichia coli, Salmonella and Bacillus subtilis and Alternaria had the strongest growth inhibitory effect on Staphylococcus aureus. The results showed that Hainan patchouli endophytic fungi had certain biodiversity and some endophytic fungal strains had more obvious antibacterial activity.

Key words: Pogostemon cablin, endophytic fungi, antagonistic strains

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