Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1590-1597.DOI: 10.3969/j.issn.1000-2561.2019.08.020
• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles Next Articles
WU Ruhui,LI Zengping(),CHENG Lele,ZHANG Yu
Received:
2018-09-18
Revised:
2019-04-24
Online:
2019-08-25
Published:
2019-08-20
Contact:
LI Zengping
CLC Number:
WU Ruhui,LI Zengping,CHENG Lele,ZHANG Yu. Identification and Biological Characteristics of Acacia confuse Root Rot Pathogen of Amauroderma[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1590-1597.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.rdzwxb.com/EN/10.3969/j.issn.1000-2561.2019.08.020
Fig. 1 Field symptoms A, B: Symptoms of field plants;C: Diseased tree xylem tissue white rot;D-F: Basidiocarps grow on the surface and the stem and stem base of diseased trees; G: The under surface of the basidiocarp turns blood red after injury.
Fig. 2 Pathogenicity test A:The leaves turn yellow and branches die after 4 months of inoculation; B:The pathogens have been colonized at the inoculation site, and the internal tissue is white rot; C: CK.
Fig. 4 Morphology of pathogen A: Pathogen’s colony morphology on PDA medium for 6 days; B: Young basidiocarp of pathogen grown on PDA medium for 90 days; C,D,E: Fresh basidiocarp; F: dried basidiocarp; G: Hyphae (①Skeletal hyphae; ②Bindinghyphae; ③Generative hyphae); H: Basidiospores.
Fig. 6 Effect of different temperature on hyphae growth of A. rugosum Different small letters indicate significant difference at 0.05 level. Different capital letters indicate significant difference at 0.01 level.
Fig. 7 Effect of different light on mycelial growth of A. rugosum Different small letters indicate significant difference at 0.05 level. Different capital letters indicate significant difference at 0.01 level.
Fig. 8 Effect of different pH on mycelial growth of A. rugosum Different small letters indicate significant difference at 0.05 level. Different capital letters indicate significant difference at 0.01 level.
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落特征 Colony characteristics |
---|---|---|
D-果糖 D-Fructose | 7.72±0.07aA | 菌丝乳白色致密,菌落近圆形,长势优良 |
可溶性淀粉 Soluble starch | 6.40±0.05bB | 菌丝乳白色,菌落扇形,长势一般 |
葡萄糖 Glucose | 6.22±0.07cC | 菌丝乳白色,菌落扇形,长势一般 |
蔗糖 Sucrose | 6.10±0.08cdCD | 菌丝乳白色,菌落近圆形,长势一般 |
麦芽糖 Malt dust | 6.00±0.05dD | 菌丝乳白色,菌落近圆形,长势一般 |
肌醇 Inositol | 5.55±0.10eE | 菌落近圆形,较透明, 长势极差 |
D-山梨醇 D-Sorbitol | 4.28±0.05gG | 菌丝乳白色,菌落扇形,长势一般 |
对照 CK | 5.28±0.07fF | 菌落不规则形,较透明, 长势极差 |
Tab. 1 Effect of different carbon sources on mycelial growth of A. rugosum
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落特征 Colony characteristics |
---|---|---|
D-果糖 D-Fructose | 7.72±0.07aA | 菌丝乳白色致密,菌落近圆形,长势优良 |
可溶性淀粉 Soluble starch | 6.40±0.05bB | 菌丝乳白色,菌落扇形,长势一般 |
葡萄糖 Glucose | 6.22±0.07cC | 菌丝乳白色,菌落扇形,长势一般 |
蔗糖 Sucrose | 6.10±0.08cdCD | 菌丝乳白色,菌落近圆形,长势一般 |
麦芽糖 Malt dust | 6.00±0.05dD | 菌丝乳白色,菌落近圆形,长势一般 |
肌醇 Inositol | 5.55±0.10eE | 菌落近圆形,较透明, 长势极差 |
D-山梨醇 D-Sorbitol | 4.28±0.05gG | 菌丝乳白色,菌落扇形,长势一般 |
对照 CK | 5.28±0.07fF | 菌落不规则形,较透明, 长势极差 |
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落特征 Colony characteristics |
---|---|---|
酵母浸膏 Yeast extract | 7.28±0.05aA | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
大豆蛋白胨 Soya peptone | 6.62±0.06bB | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
L-天冬酰胺 L-Asparagine | 6.35±0.03cC | 菌落乳白色,菌落扇形,长势一般 |
牛肉浸膏 Beef extract | 5.92±0.07dD | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
硝酸钙 Calcium nitrate | 5.77±0.07deDE | 菌丝白色不规则,长势差 |
硝酸铵 Ammonium nitrate | 5.76±0.02defDEF | 菌丝白色,菌落不规则形,长势差 |
硝酸钾 Potassium nitrate | 5.71±0.07efgDEFG | 菌丝层较厚且厚薄均匀,长势优良 |
硫酸铵 Ammonium sulphate | 5.70±0.05fghEFG | 菌丝白色扇形,长势差 |
硝酸钠 Sodium nitrate | 5.67±0.03fghFG | 菌丝白色,菌落近圆形,长势差 |
氯化铵 Ammonium chloride | 5.55±0.08ghFG | 菌丝层近透明;长势差 |
草酸铵 Ammonium oxalate | 5.41±0.06hG | 菌丝白色,菌落雪花状,长势较差 |
对照 CK | 5.58±0.06iH | 菌丝白色不规则,长势差 |
Tab. 2 Effect of different nitrogen sources on mycelial growth of A. rugosum
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落特征 Colony characteristics |
---|---|---|
酵母浸膏 Yeast extract | 7.28±0.05aA | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
大豆蛋白胨 Soya peptone | 6.62±0.06bB | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
L-天冬酰胺 L-Asparagine | 6.35±0.03cC | 菌落乳白色,菌落扇形,长势一般 |
牛肉浸膏 Beef extract | 5.92±0.07dD | 菌落近圆形,中心深褐色边缘乳白色,菌丝致密 |
硝酸钙 Calcium nitrate | 5.77±0.07deDE | 菌丝白色不规则,长势差 |
硝酸铵 Ammonium nitrate | 5.76±0.02defDEF | 菌丝白色,菌落不规则形,长势差 |
硝酸钾 Potassium nitrate | 5.71±0.07efgDEFG | 菌丝层较厚且厚薄均匀,长势优良 |
硫酸铵 Ammonium sulphate | 5.70±0.05fghEFG | 菌丝白色扇形,长势差 |
硝酸钠 Sodium nitrate | 5.67±0.03fghFG | 菌丝白色,菌落近圆形,长势差 |
氯化铵 Ammonium chloride | 5.55±0.08ghFG | 菌丝层近透明;长势差 |
草酸铵 Ammonium oxalate | 5.41±0.06hG | 菌丝白色,菌落雪花状,长势较差 |
对照 CK | 5.58±0.06iH | 菌丝白色不规则,长势差 |
[1] | 吕春 . 台湾相思树[J]. 甘肃林业, 2010(5):44. |
[2] | 沙桦欣, 伍建榕, 周利平 , 等. 干热河谷台湾相思树种根瘤菌的耐干热研究[J]. 中南林业科技大学学报, 2013,33(8):63-67, 73. |
[3] | 黄宝灵, 吕成群, 韦原莲 , 等. 相思树种根瘤菌的若干抗逆特性[J]. 南京林业大学学报(自然科学版), 2004(1):29-32. |
[4] | 张一帆, 谢意珍, 杨小兵 . 假芝功效研究进展[J]. 食用菌, 2018,40(2):1-4. |
[5] | 范藜, 刘波 . 假芝属一新种[J]. 真菌学报, 1990,9(3):202-205. |
[6] | 赵继鼎, 张小青 . 中国灵芝科真菌资源与分布[J]. 真菌学报, 1992,11(l):55-62. |
[7] | 赵继鼎, 张小青 . 中国真菌志: 第18卷灵芝科[M]. 北京: 科学出版社, 2000: 144-183, 384-385. |
[8] | 秦改娟, 王珊珊, 陈青君 , 等. 一株假芝的分离鉴定与生物学特性[J]. 应用与环境生物学报, 2015,21(3):464-469. |
[9] | 戴玉成, 吴兴亮, 魏玉莲 , 等. 中国海南台湾相思树干基腐朽病[J]. 林业科学研究, 2004(3):352-355. |
[10] | 谭象生, 汪国声 . 台湾相思的一种新病害——根腐病[J]. 林业科技通讯, 1984(9):29. |
[11] | 方中达 . 植病研究方法[M]. 北京: 中国农业出版社, 1998: 122-127. |
[12] | 高秀兵, 李增平, 李晓娜 , 等. 橡胶树几种根病的人工接种方法[J]. 热带作物学报, 2010,31(4):626-630. |
[13] | 卯晓岚 . 中国大型真菌[M]. 郑州: 河南科学技术出版社, 2000: 450. |
[14] | 吴兴亮, 戴玉成, 李泰辉 , 等. 中国热带真菌[M]. 北京: 科学出版社, 2011: 322-327. |
[15] | White T, Bruns T, Lee S , et al. 38-Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Innis M A, Gelfand D H, Sninsky J, et al. PCR protocols: a guide to methods and applications. San Diego: Academic Press, 1990: 315-322. |
[16] | Tamura K, Stecher G, Peterson D , et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 2013,30(12):2725-2729. |
[17] | 肖自添, 刘明, 何焕清 . 一株野生乌芝的鉴定及其生物学特性研究[J]. 广东农业科学, 2016,43(3):72-76, 2. |
[18] | Jiao C W, Xie Y Z, Yang X L , et al. Anticancer Activity of Amauroderma rude[J]. PLoS One, 2013,8(6):e66504. |
[19] | 吴兴亮, 戴玉成, 林尤河 . 灵芝科资源及其孢子扫描电镜观察研究[J]. 贵州科学, 2005,23(2):33-39. |
[1] | WANG Qinglong,WANG Maoyuan,YANG Hubiao,YAN Xiaoxia,WANG Zhunian. Newly Recorded Plants of Angiosperm in Hainan (Ⅲ) [J]. Chinese Journal of Tropical Crops, 2020, 41(7): 1484-1489. |
[2] | CHE Haiyan,CAO Xueren,HE Yanheng,LUO Daquan. Distribution and Identification of Viruses from Cucumber in Hainan Island [J]. Chinese Journal of Tropical Crops, 2020, 41(11): 2280-2284. |
[3] | HAO Huihua,LI Guoyin,CUI Zhifu,SUN Junmei,ZHAN Daoyi,ZHANG Fangping,PENG Zhengqiang. Investigation on Predatory Mites in Rubber Trees and Evaluation of Dominant Species of Predatory Mites in Hainan [J]. Chinese Journal of Tropical Crops, 2020, 41(1): 141-147. |
[4] | LU Hui,TANG Jihong,LYU Baoqian,MA Zilong,HE Xing,CHEN Qi,SU Hao. Recent Advances in Biological Control and Invasion Risk of Spodoptera frugiperda [J]. Chinese Journal of Tropical Crops, 2019, 40(6): 1237-1244. |
[5] | HE Chunping,LI Rui,LIANG Yanqiong,WU Weihuai,HUANG Xing,XI Jingen,ZHENG Jinlong,YI Kexian. Biological Characteristics and Toxicity Test of Pathogen Strains Causing Rubber Red Root Rot from Different Regions [J]. Chinese Journal of Tropical Crops, 2019, 40(3): 522-529. |
[6] | WU Lin,PENG Lixu,LIU Beibei,PAN Pan,CHEN Xin,LI Qinfen. Intercropping Performance of Five New Yam Varieties in Young Rubber Plantation in Hainan [J]. Chinese Journal of Tropical Crops, 2019, 40(2): 209-214. |
[7] | WU Ruhui,LI Zengping,CHEN Lilang. Identification and Biological Characteristics of the Pathogen Causing Stem Rot on Casuarina equisetifolia [J]. Chinese Journal of Tropical Crops, 2019, 40(2): 334-340. |
[8] | YE Kang. Newly Recorded Species of Orchidaceae in Hainan, China [J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2261-2263. |
[9] | XUE Yang,SU Shaofeng,LIN Zhipan,WANG Xiaoyan,CHEN Yiqing,XUE Yanwen,CHEN Pingmei. Soil Organic Carbon Distribution in Five Forest Types in the Northeast of Hainan Island [J]. Chinese Journal of Tropical Crops, 2019, 40(11): 2294-2299. |
[10] | CHEN Zongzhu, CHEN Yiqing, YANG Qi, CHEN Xiaohua, LEI Jinrui. Compatibility Biomass Model of Eucalyptus in Hainan Island [J]. Chinese Journal of Tropical Crops, 2018, 39(9): 1868-1875. |
[11] | HE Cuicui, FENG Huande, WEI Zhiyuan, HOU Xianwen, CHEN Yeyuan. Investigation of Current Situation of Fertilization and Analysis of Soil Nutrients of Mango Orchard in Hainan Island [J]. Chinese Journal of Tropical Crops, 2018, 39(7): 1266-1275. |
[12] | WU Ruhui, LI Zengping, SHUNXIAN Yiqing, ZHANG Yu, DING Jingyu, CHENG Lele. Identification and Biological Characteristics of the Pathogen Causing Stinking Root Rot of Rubber Tree [J]. Chinese Journal of Tropical Crops, 2018, 39(5): 940-947. |
[13] | XING Wenting XU Yi CHEN Pei DU Limin CHEN Xianzhen. Introduction Cultivation and Growth Characteristics of Cinnamomum kanehirae Hayata (Lauraceae) in Hainan [J]. Chinese Journal of Tropical Crops, 2018, 39(4): 630-634. |
[14] | DING Jingyu, LI Zengping. Identification and Biological Characteristics of Salix babylonica Stem Rot Pathogen [J]. Chinese Journal of Tropical Crops, 2018, 39(3): 547-552. |
[15] | HE Cuicui , FENG Huande , WEI Zhiyuan, HOU Xianwen , CHEN Yeyuan. Investigation of Current Situation of Fertilization and Analysis of Soil Nutrients of Mango Orchard in Hainan Island [J]. Chinese Journal of Tropical Crops, 2018, 39(12): 2336-2342. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||