Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1577-1584.DOI: 10.3969/j.issn.1000-2561.2019.08.018

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Identification of Viruses Infecting Passiflora edulis in Guizhou Province by Small RNA Sequencing

YAN Jiawen1,YUAN Qifeng1,XIE Pu1,WANG Lijuan1,MA Yuhua1,*(),WANG Zhengyuan2   

  1. 1. Institute of Pomology, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China
    2. Fruit Industry Development Office of Luodian County, Luodian, Guizhou 550100, China
  • Received:2018-11-28 Revised:2019-01-29 Online:2019-08-25 Published:2019-08-20
  • Contact: MA Yuhua


Virus disease is one of the main diseases of Passiflora edulis, and the identification of virus species can provide a theoretical basis for the disease prevention. In this study, six suspected virus-infected leaf samples were mixed and analyzed by small RNA deep sequencing. Cucumber mosaic virus (CMV) and Telosma mosaic virus (TeMV) were detected from the mixed sample. Viruses identified by small RNA sequencing were validated by virus coat protein gene (CP) specific RT-PCR from the above six samples. CMV and TeMV was detected from all six samples, respectively. The open reading frame of CP gene of CMV and TeMV obtained by RT-PCR was 657 bp and 816 bp, correspondingly. The nucleotide sequence identity of CMV-GZ (accession number: MH623077), TeMV-GZ1 (accession number: MH623078) and TeMV-GZ2 (accession number: MH623079) was 82.3%-95.9%, 87.8%-99.3% and 87.8%-98.3%, respectively, with the reported CMV and TeMV isolates. CMV and TeMV in P. edulis was first reported Guizhou. The combined infection of the two viruses may be a potential threat to P. edulis.

Key words: Passiflora edulis, virus, small RNA sequencing, identification

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