Toxicity of Insecticides Against Larvae of Spodoptera frugiperda Population, A New Invasive Pest

doi:10.3969/j.issn.1000-2561.2019.08.017

Abstract

Abstract:

The fall armyworm, Spodoptera frugiperda, first invaded Yunnan, China, in January 2019, is a notorious agricultural pest insects. It was first founded on maize in Haikou city in April, and poses a serious threat to the production of fresh eating maize. It is urgent to screen out high toxicity insecticides for controlling this pest. By leaf dipping method, the toxicities of 15 insecticides to larvae of S. frugiperda were tested under laboratory conditions. The results showed that at the relative lower concentration of emamectin benzoate (5 mg/L, 24 h) and spinosad (5 mg/L, 24 h), and relative higher concentration of chlorantraniliprole (50 mg/L, 72 h) and phoxim (100 mg/L, 72 h) have 100% contact toxicity to larvae of S. frugiperda. Their 50% lethal concentration (LC₅₀) at 24 h is 0.28, 0.31, 9.77 and 11.7 mg/L, respectively. Other insecticides such as methomy, pyriproxyfen, fipronil, acetamiprid, abamectin, beta-cypermethrin and lufenuron, at concentration of 100 mg/L with 72 h, have 100%, 96.67%, 88.33%, 86.11%, 81.67%, 80.83%, and 80.09% contact toxicity to larvae of S. frugiperda. It recommended to use emamectin benzoate, spinosad, chlorantraniliprole, and phoxim as the main ingredients, as well as combined with other above mentioned insecticides, for controlling of larvae of S. frugiperda.

Key words: Spodoptera frugiperda, insecticide, toxicity bioassay, leaf dipping method

CLC Number:

Q968.1

JIN Tao,LIN Yuying,MA Guangchang,MA Zilong,XIANG Kaiping,YI Kexian,PENG Zhengqiang. Toxicity of Insecticides Against Larvae of Spodoptera frugiperda Population, A New Invasive Pest[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1571-1576.

Figures/Tables 2

References 22

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药剂 Insecticides	浓度 Concentrations/(mg·L^-1)	24 h	48 h	72 h
甲维盐 Emamectin benzoate	100	100	100	100
	50	100	100	100
	10	100	100	100
	5	100	100	100
多杀菌素 Spinosad	100	100	100	100
	50	100	100	100
	10	100	100	100
	5	100	100	100
氯虫苯甲酰胺 Chlorantraniliprole	100	100^a	100^a	100^a
	50	89.63±5.79^a	100^a	100^a
	10	53.37±1.71^b	66.77±1.84^b	96.67±3.33^a
	5	11.67±0.83^c	58.33±4.17^b	92.50±3.82^a
辛硫磷 Phoxim	100	100^a	100^a	100^a
	50	76.67±18.56^ab	86.67±13.33^a	93.33±6.67^a
	10	32.95±3.99^bc	49.62±7.55^b	79.55±7.95^a
	5	20±10^c	41.48±1.48^b	76.30±8.54^a
吡丙醚 Pyriproxyfen	100	90.00±5.77^a	96.67±3.33^a	96.67±3.33^a
	50	20.13±1.17^b	30.20±±1.75^b	40.27±2.34^b
	10	4.17±4.17^bc	12.04±0.46^c	32.41±5.09^b
	5	0^c	13.3^c	20.00±5.77^c
药剂 Insecticides	浓度 Concentrations/(mg·L^-1)	24 h	48 h	72 h
虱螨脲 Lufenuron	100	31.94±3.67^a	74.54±8.58^a	80.09±3.79^a
	50	11.67±0.83^b	35.0±2.5^b	78.33±9.17^a
	10	7.87±3.96^b	27.31±5.09^b	46.30±1.85^b
	5	0^b	7.41±7.41^b	25.93±3.70^b
高效氯氰菊酯 Beta-cypermethrin	100	20.56±2.42^a	34.17±4.64^a	80.83±3.63^a
	50	15.08±0.79^a	30.16±8.40^a	64.29±7.14^a
	10	12.63±0.92^ab	24.67±6.81^a	37.90±2.76^b
	5	3.33±3.33^b	22.41±1.45^a	22.41±1.45^b
功夫菊酯 Cyhalothrin	100	21.48±0.74^a	39.26±3.23^a	60.37±4.81^a
	50	11.50±1.51^b	18.23±2.23^b	49.01±4.08^a
	10	11.11±1.39^b	18.06±3.67^b	41.67±11.02^a
	5	0^c	3.33±3.33^c	35.56±2.22^a
吡虫啉 Imidacloprid	100	0	24.81±2.59^a	53.71±1.92^a
	50	0	24.07±3.03^a	41.52±1.50^ab
	10	0	18.15±4.07^a	35.56±2.52^b
	5	0	16.67±3.33^a	30.0±5.8^b
啶虫脒 Acetamiprid	100	25^a	80.56±2.78^a	86.11±2.78^a
	50	15.28±3.49^b	26.39±6.94^b	69.44±2.78^b
	10	12.04±0.46^b	24.07±0.93^b	48.15±1.85^c
	5	0^c	4.17±4.17^c	45.83±4.17^c
敌百虫 Trichlorfon	100	3.33±3.33^a	40.36±11.08^a	60.95±19.54^a
	50	0^a	46.7±5.7^a	56.93±3.94^a
	10	0^a	10.74±0.37^b	39.63±8.02^a
	5	0^a	12.04±4.60^b	20.37±4.17^a
灭多威 Methomy	100	54.85±2.89^a	67.88±2.12^a	100^a
	50	10.74±0.37^b	25.19±4.12^b	68.52±9.26^b
	10	10.87±1.78^b	21.75±3.54^b	66.59±4.15^b
	5	0^c	19.17±3.63^b	58.33±4.17^b
氟虫腈 Fipronil	100	23.33±7.41^a	58.33±4.17^a	88.33±7.26^a
	50	21.48±0.74^a	53.70±1.85^a	82.22±3.39^a
	10	11.50±1.51^a	42.28±7.48^a	59.45±3.68^b
	5	7.41±3.70^a	49.83±19.61^a	38.05±3.32^c
阿维菌素 Abamectin	100	31.67±4.17^a	66.67±4.17^a	81.67±5.83^a
	50	20.40±1.21^b	30.22±1.75^b	50.34±2.92^b
	10	3.33±3.33^c	29.44±2.42^b	39.17±5.83^b
	5	3.7±3.7^c	32.22±1.11^b	42.96±1.48^b
杀螟丹 Cartap	100	6.67±6.67^a	21.48±0.74^a	53.70±1.85^a
	50	3.7±3.7^a	18.52±0.37^a	40.74±3.70^ab
	10	8.3±4.2^a	15.28±3.49^ab	23.15±6.48^bc
	5	0^a	3.7±3.7^b	11.11^c

药剂 Insecticides	浓度 Concentrations/(mg·L^-1)	24 h	48 h	72 h
甲维盐 Emamectin benzoate	100	100	100	100
	50	100	100	100
	10	100	100	100
	5	100	100	100
多杀菌素 Spinosad	100	100	100	100
	50	100	100	100
	10	100	100	100
	5	100	100	100
氯虫苯甲酰胺 Chlorantraniliprole	100	100^a	100^a	100^a
	50	89.63±5.79^a	100^a	100^a
	10	53.37±1.71^b	66.77±1.84^b	96.67±3.33^a
	5	11.67±0.83^c	58.33±4.17^b	92.50±3.82^a
辛硫磷 Phoxim	100	100^a	100^a	100^a
	50	76.67±18.56^ab	86.67±13.33^a	93.33±6.67^a
	10	32.95±3.99^bc	49.62±7.55^b	79.55±7.95^a
	5	20±10^c	41.48±1.48^b	76.30±8.54^a
吡丙醚 Pyriproxyfen	100	90.00±5.77^a	96.67±3.33^a	96.67±3.33^a
	50	20.13±1.17^b	30.20±±1.75^b	40.27±2.34^b
	10	4.17±4.17^bc	12.04±0.46^c	32.41±5.09^b
	5	0^c	13.3^c	20.00±5.77^c
药剂 Insecticides	浓度 Concentrations/(mg·L^-1)	24 h	48 h	72 h
虱螨脲 Lufenuron	100	31.94±3.67^a	74.54±8.58^a	80.09±3.79^a
	50	11.67±0.83^b	35.0±2.5^b	78.33±9.17^a
	10	7.87±3.96^b	27.31±5.09^b	46.30±1.85^b
	5	0^b	7.41±7.41^b	25.93±3.70^b
高效氯氰菊酯 Beta-cypermethrin	100	20.56±2.42^a	34.17±4.64^a	80.83±3.63^a
	50	15.08±0.79^a	30.16±8.40^a	64.29±7.14^a
	10	12.63±0.92^ab	24.67±6.81^a	37.90±2.76^b
	5	3.33±3.33^b	22.41±1.45^a	22.41±1.45^b
功夫菊酯 Cyhalothrin	100	21.48±0.74^a	39.26±3.23^a	60.37±4.81^a
	50	11.50±1.51^b	18.23±2.23^b	49.01±4.08^a
	10	11.11±1.39^b	18.06±3.67^b	41.67±11.02^a
	5	0^c	3.33±3.33^c	35.56±2.22^a
吡虫啉 Imidacloprid	100	0	24.81±2.59^a	53.71±1.92^a
	50	0	24.07±3.03^a	41.52±1.50^ab
	10	0	18.15±4.07^a	35.56±2.52^b
	5	0	16.67±3.33^a	30.0±5.8^b
啶虫脒 Acetamiprid	100	25^a	80.56±2.78^a	86.11±2.78^a
	50	15.28±3.49^b	26.39±6.94^b	69.44±2.78^b
	10	12.04±0.46^b	24.07±0.93^b	48.15±1.85^c
	5	0^c	4.17±4.17^c	45.83±4.17^c
敌百虫 Trichlorfon	100	3.33±3.33^a	40.36±11.08^a	60.95±19.54^a
	50	0^a	46.7±5.7^a	56.93±3.94^a
	10	0^a	10.74±0.37^b	39.63±8.02^a
	5	0^a	12.04±4.60^b	20.37±4.17^a
灭多威 Methomy	100	54.85±2.89^a	67.88±2.12^a	100^a
	50	10.74±0.37^b	25.19±4.12^b	68.52±9.26^b
	10	10.87±1.78^b	21.75±3.54^b	66.59±4.15^b
	5	0^c	19.17±3.63^b	58.33±4.17^b
氟虫腈 Fipronil	100	23.33±7.41^a	58.33±4.17^a	88.33±7.26^a
	50	21.48±0.74^a	53.70±1.85^a	82.22±3.39^a
	10	11.50±1.51^a	42.28±7.48^a	59.45±3.68^b
	5	7.41±3.70^a	49.83±19.61^a	38.05±3.32^c
阿维菌素 Abamectin	100	31.67±4.17^a	66.67±4.17^a	81.67±5.83^a
	50	20.40±1.21^b	30.22±1.75^b	50.34±2.92^b
	10	3.33±3.33^c	29.44±2.42^b	39.17±5.83^b
	5	3.7±3.7^c	32.22±1.11^b	42.96±1.48^b
杀螟丹 Cartap	100	6.67±6.67^a	21.48±0.74^a	53.70±1.85^a
	50	3.7±3.7^a	18.52±0.37^a	40.74±3.70^ab
	10	8.3±4.2^a	15.28±3.49^ab	23.15±6.48^bc
	5	0^a	3.7±3.7^b	11.11^c

杀虫剂Insecticide	致死中浓度 LC₅₀(95% CI)/(mg·L^-1)	致死90%浓度 LC₉₀/(mg·L^-1)	斜率 Slop(±SE)	χ²	决定系数 r²
甲维盐Emamectin benzoate	0.28(0.1~0.46)^a	3.32	1.19(±0.25)	0.24	0.99
多杀菌素Spinosad	0.31(0.1~0.51)^a	3.12	1.27(±0.30)	0.97	0.94
氯虫苯甲酰胺Chlorantraniliprole	9.77(6.78~13.44)^b	61.81	1.60(±0.28)	2.76	0.92
辛硫磷Phoxim	11.7(9.18~14.91)^b	39.56	2.42(±0.34)	3.17	0.96

杀虫剂Insecticide	致死中浓度 LC₅₀(95% CI)/(mg·L^-1)	致死90%浓度 LC₉₀/(mg·L^-1)	斜率 Slop(±SE)	χ²	决定系数 r²
甲维盐Emamectin benzoate	0.28(0.1~0.46)^a	3.32	1.19(±0.25)	0.24	0.99
多杀菌素Spinosad	0.31(0.1~0.51)^a	3.12	1.27(±0.30)	0.97	0.94
氯虫苯甲酰胺Chlorantraniliprole	9.77(6.78~13.44)^b	61.81	1.60(±0.28)	2.76	0.92
辛硫磷Phoxim	11.7(9.18~14.91)^b	39.56	2.42(±0.34)	3.17	0.96