Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1565-1570.DOI: 10.3969/j.issn.1000-2561.2019.08.016
• Biotechnology and Tissue Culture • Previous Articles Next Articles
SU Fan1,2,YANG Xiaobo1,*(),LI Donghai1
Received:
2018-09-28
Revised:
2019-05-02
Online:
2019-08-25
Published:
2019-08-20
Contact:
YANG Xiaobo
CLC Number:
SU Fan,YANG Xiaobo,LI Donghai. The Classification Status of Wuzhishan Wild Tea Based on Morphological Features and psbA-trnH Chloroplast Encoding Gene Sequence[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1565-1570.
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位置Locality | 取样数Number | 经度Longitude | 纬度Latitude | 海拔Altitude/m |
---|---|---|---|---|
畅好什冲岭 | 10份 | 109°26’11.72E | 18°4336.77N | 735 |
南圣毛祥后山1 | 10份 | 109°3915.0E | 18°4748.6N | 625 |
南圣毛祥后山2 | 10份 | 109°3916.9E | 18°4722.6N | 563 |
钟山番赛村后山 | 10份 | 109°3251.94E | 18°4951.18N | 1039 |
毛道新泰南岭 | 10份 | 109°235.9E | 18°4253.9N | 371 |
水满水满村后山 | 10份 | 109°4234.7E | 18°504.0N | 1122 |
水满东松岭后山 | 10份 | 109°4136.6E | 18°5252.5N | 1031 |
水满仕阶老村后山 | 10份 | 109°4510.12E | 18°5415.91N | 1102 |
Tab. 1 Populations of wild tea in Wuzhishan
位置Locality | 取样数Number | 经度Longitude | 纬度Latitude | 海拔Altitude/m |
---|---|---|---|---|
畅好什冲岭 | 10份 | 109°26’11.72E | 18°4336.77N | 735 |
南圣毛祥后山1 | 10份 | 109°3915.0E | 18°4748.6N | 625 |
南圣毛祥后山2 | 10份 | 109°3916.9E | 18°4722.6N | 563 |
钟山番赛村后山 | 10份 | 109°3251.94E | 18°4951.18N | 1039 |
毛道新泰南岭 | 10份 | 109°235.9E | 18°4253.9N | 371 |
水满水满村后山 | 10份 | 109°4234.7E | 18°504.0N | 1122 |
水满东松岭后山 | 10份 | 109°4136.6E | 18°5252.5N | 1031 |
水满仕阶老村后山 | 10份 | 109°4510.12E | 18°5415.91N | 1102 |
茶(原变种) C. sinensis | 白毛茶 C. sinensis var. pubilimba | 普洱茶 C. sinensis var. assamica | 五指山野生茶 Wuzhishan wild tea | |
---|---|---|---|---|
叶 | 叶片下面无毛或初时有柔毛;边缘有锯齿。 | 嫩枝及叶片下面均被有密柔毛;叶边缘锯齿不明显;叶柄无毛。 | 嫩枝有微毛,顶芽有白柔毛;侧脉8~9对,叶边缘有细锯齿;叶柄被柔毛。 | 侧脉11~13对,有不显著齿或波状齿,老叶锯齿深明,常大小相间;叶柄被柔毛。 |
花 | 花腋生,白色;萼片无毛。 | 花腋生,白色,花特别小,萼片被灰白色 | 花腋生,白色,花柄被柔毛;萼片无毛;花瓣6~7片,背面无毛。 | 花腋生,白色,花柄被柔毛;萼片无毛;花瓣5片,倒卵形,有时为披尖形。 |
果实 | 蒴果3球形或1~2球形,每球有种子1~2粒。花期10月至翌年2月。 | 蒴果3球形或1~2球形,每室有种子1~2粒。 | 蒴果扁三角球形,3爿裂开,种子每室1个。 | 蒴果扁三角球形或1个圆球形,3爿裂开,种子每室1个。 |
Tab. 2 Comparison of the morphological characteristics of three kinds of tea
茶(原变种) C. sinensis | 白毛茶 C. sinensis var. pubilimba | 普洱茶 C. sinensis var. assamica | 五指山野生茶 Wuzhishan wild tea | |
---|---|---|---|---|
叶 | 叶片下面无毛或初时有柔毛;边缘有锯齿。 | 嫩枝及叶片下面均被有密柔毛;叶边缘锯齿不明显;叶柄无毛。 | 嫩枝有微毛,顶芽有白柔毛;侧脉8~9对,叶边缘有细锯齿;叶柄被柔毛。 | 侧脉11~13对,有不显著齿或波状齿,老叶锯齿深明,常大小相间;叶柄被柔毛。 |
花 | 花腋生,白色;萼片无毛。 | 花腋生,白色,花特别小,萼片被灰白色 | 花腋生,白色,花柄被柔毛;萼片无毛;花瓣6~7片,背面无毛。 | 花腋生,白色,花柄被柔毛;萼片无毛;花瓣5片,倒卵形,有时为披尖形。 |
果实 | 蒴果3球形或1~2球形,每球有种子1~2粒。花期10月至翌年2月。 | 蒴果3球形或1~2球形,每室有种子1~2粒。 | 蒴果扁三角球形,3爿裂开,种子每室1个。 | 蒴果扁三角球形或1个圆球形,3爿裂开,种子每室1个。 |
Fig. 2 Rich morphological variation of the wild tea communities in Wuzhishan A, C, D, F were taken in Shichong Mountain, Changhao village. B, E, G were taken in Dongsong Mountain, Shuiman village.
名称 Name | 扩增引物 Amplimer | PCR反应条件 Reaction condition | 测序结果 Sequencing analysis | |
---|---|---|---|---|
rbcL | 1F: ATGTCACCACAAACAGAGACTAAAGC | 95 ℃ 4 min;94 ℃ 30 s;54 ℃ 1 min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序失败 | |
724R: TCGCATGTACCTGCAGTAGC | ||||
matK | 3F: CGTACAGTACTTTTGTGTTTACGAG | 95 ℃ 4 min;94 ℃ 30 s;51 ℃ 1 min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序失败 | |
1R: ACCCAGTCCATCTGGAAATCTTGGTTC | ||||
psbA-trnH | trnH: CGCGCATGGTGGATTCACAATCC | 95 ℃ 4 min;94 ℃ 30 s;55 ℃ 1min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序成功 | |
psbA: GTTATGCATGAACGTAAT GCTC | ||||
ITS | ITS4: TCCTCCGCTTATTGATATGC | 95 ℃ 4 min;94 ℃ 30 s;50 ℃ 1 min;72 ℃1 min;35次循环;72 ℃ 10 min | 测序失败 | |
ITS5: GGAAGTAAAAGTCGTAACAAGG |
Tab. 3 Universal primers and reaction conditions for candidate barcodes
名称 Name | 扩增引物 Amplimer | PCR反应条件 Reaction condition | 测序结果 Sequencing analysis | |
---|---|---|---|---|
rbcL | 1F: ATGTCACCACAAACAGAGACTAAAGC | 95 ℃ 4 min;94 ℃ 30 s;54 ℃ 1 min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序失败 | |
724R: TCGCATGTACCTGCAGTAGC | ||||
matK | 3F: CGTACAGTACTTTTGTGTTTACGAG | 95 ℃ 4 min;94 ℃ 30 s;51 ℃ 1 min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序失败 | |
1R: ACCCAGTCCATCTGGAAATCTTGGTTC | ||||
psbA-trnH | trnH: CGCGCATGGTGGATTCACAATCC | 95 ℃ 4 min;94 ℃ 30 s;55 ℃ 1min;72 ℃ 1 min;35次循环;72 ℃ 10 min | 测序成功 | |
psbA: GTTATGCATGAACGTAAT GCTC | ||||
ITS | ITS4: TCCTCCGCTTATTGATATGC | 95 ℃ 4 min;94 ℃ 30 s;50 ℃ 1 min;72 ℃1 min;35次循环;72 ℃ 10 min | 测序失败 | |
ITS5: GGAAGTAAAAGTCGTAACAAGG |
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