Establishment of a Tissue Culture System for Wild <i>Polygonatum cyrtonema</i> Hua in Sanming
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1559-1564.DOI: 10.3969/j.issn.1000-2561.2019.08.015

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Establishment of a Tissue Culture System for Wild Polygonatum cyrtonema Hua in Sanming

LYU Yumeng1,XU Xiaoping1,ZHANG Shuting1,GUO Zhipeng2,WANG Jinfeng2,LIN Yuling1,WANG Tianchi1,*(),LAI Zhongxiong1,*()   

  1. 1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Fujian Pangu Traditional Chinese Medicine Co., Ltd., Sanming, Fujian 365000, China
  • Received:2018-11-24 Revised:2019-04-10 Online:2019-08-25 Published:2019-08-20
  • Contact: WANG Tianchi,LAI Zhongxiong


An aseptic tissue culture procedure was established for wild Polygonatum cyrtonema Hua in Samming by optimizing the disinfection and the adventitious bud induction procedures, in which the tubers from wild-growing plants were used as the explants. The best sterilization procedure for the explants was as follows: brushed soils off the tubers in clear water with a soft toothbrush; cleaned within diluted detergent for 16 hours; soaked in 3% carbendazim for 2 days (shaked intermittently) and washed in water to remove the residual carbendazim from the surface; dried on filter paper for 5 h; dipped first in 75% alcohol for 30 s and then in 0.2% mercuric chloride for 15 min, which resulted in a contamination rate of only 20.2%. Comparatively, the explants from March to April were significantly better than those from November to December in both the growth and the induction of adventitious buds. The optimal medium for the adventitious bud induction of the wild P. cyrtonema Hua explants was: MS+6-BA 4.0 mg/L +NAA 0.2 mg/L, and the budding rate on the medium was as high as 88.0%.

Key words: wild Polygonatum cyrtonema Hua, sterile system, tissue culture, adventitious bud induction

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