An Open <i>in vitro</i> Culture System for Rapid Propagation of Cut Flower <i>Chrysanthemum</i> cv Iwanohakusen
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1551-1558.DOI: 10.3969/j.issn.1000-2561.2019.08.014

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

An Open in vitro Culture System for Rapid Propagation of Cut Flower Chrysanthemum cv Iwanohakusen

ZHU Mengzhu1,YANG Huiting1,HU Jihong1,TAN Yanli1,HONG Rongqin2,LIN Qingliang1,PAN Dongming1,KANG Jianban3,SHE Wenqin1,*(),CHEN Guixin1,*()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Youxi Sanye Flower Co. Ltd., Youxi, Fujian 365100, China
    3. Institute of Crops, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
  • Received:2018-07-19 Revised:2019-03-20 Online:2019-08-25 Published:2019-08-20
  • Contact: SHE Wenqin,CHEN Guixin


An open in vitro culture system was established for the rapid propagation of cut flower Chrysanthemum ‘Iwanohakusen’, in which the best explant surface sterilization agent and the best microorganism growth inhibiting agents were screened out. The best explant surface sterilization was obtained by gently shaking the explants in 0.1% (V/V) of sodium hypochlorite for 15 min. The best result for inhibiting the growth of microorganisms in the primary culture stage was achieved by adding 50 mg/L of mancozeb, 0.1% (V/V) of sodium hypochlorite and 5 mg/L of potassium sorbate, into the medium, and under the condition the contamination rate was as low as 36.7%. There was no significant differences in the induction and the growth rates of the adventitious buds between the traditional tissue culture system and the open culture system. The best combination of the microorganism growth inhibiting agents was 40 mg/L of mancozeb, 0.1% (V/V) sodium hypochlorite and 5 mg/L of potassium sorbate at the stages of subculture and the rooting in the open system, and a high bud multiplication efficiency of 5.93% and a rooting rate of 80.0% were achieved at the condition, without any significant difference in bud growth compared to the conventional tissue culture system.

Key words: cut flower Chrysanthemum ‘Iwanohakusen’ (Chrysanthemum cv. Iwanohakusen), open rapid propagation in vitro, bacteriostatic agents

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