Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1546-1550.DOI: 10.3969/j.issn.1000-2561.2019.08.013

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cytological Analysis on Fertility of Triploid Salvia miltiorrhiza

ZHANG Nixuan,ZHANG Luyao,CAO Yannan,ZHENG Guiheng,LONG Hong()   

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2018-09-08 Revised:2019-03-19 Online:2019-08-25 Published:2019-08-20
  • Contact: LONG Hong


Triploid Salvia, with characters of sterility, was obtained through hybridization of diploid and tetraploid. In order to investigate the cytological mechanisms of its sterility, we observed the chromosome change and cell structure during the development of pollen mother cells. Results showed the triploid plants grew well. Numerous meiotic abnormalities, including lagging chromosomes, asymmetrical separation and chromosome bridges were found during the meiosis process. Micronuclei and triads were observed except tetrads. Moreover, the shape of the pollen was abnormal, and the pollen germination rate was very low. This indicated that the chromosomal and cytological abnormalities in the meiotic process due to chromosome number imbalance may lead to sterility in triploid Salvia. The data could provide experimental supports of fertility of triploid Salvia, and be useful in the breeding of the traditional Chinese medical herb.

Key words: triploid, fertility, pollen mother cell, meiosis, Salvia miltiorrhiza

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