Cytological Analysis on Fertility of Triploid Salvia miltiorrhiza

doi:10.3969/j.issn.1000-2561.2019.08.013

Abstract

Abstract:

Triploid Salvia, with characters of sterility, was obtained through hybridization of diploid and tetraploid. In order to investigate the cytological mechanisms of its sterility, we observed the chromosome change and cell structure during the development of pollen mother cells. Results showed the triploid plants grew well. Numerous meiotic abnormalities, including lagging chromosomes, asymmetrical separation and chromosome bridges were found during the meiosis process. Micronuclei and triads were observed except tetrads. Moreover, the shape of the pollen was abnormal, and the pollen germination rate was very low. This indicated that the chromosomal and cytological abnormalities in the meiotic process due to chromosome number imbalance may lead to sterility in triploid Salvia. The data could provide experimental supports of fertility of triploid Salvia, and be useful in the breeding of the traditional Chinese medical herb.

Key words: triploid, fertility, pollen mother cell, meiosis, Salvia miltiorrhiza

CLC Number:

Q945.53

ZHANG Nixuan,ZHANG Luyao,CAO Yannan,ZHENG Guiheng,LONG Hong. Cytological Analysis on Fertility of Triploid Salvia miltiorrhiza[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1546-1550.

Figures/Tables 5

Fig. 1 Morphology of triploid Salvia miltiorrhiza A: Plants with flower, around 3 months old; B: Inflorescence with multi-flowers, bluish violet corolla; C: Stamen, two normal-like stamen; D: Ovary, two carpel.

Fig. 2 Chromosome behavior during meiosis of triploid Salvia miltiorrhiza A: Trivalent, two valent and univalent, arrows indicate bivalent, *indicate monovalent, # indicate trivalent; B: Lagging chromosome; C: Asymmetrical separation; D: Chromosome bridges.

Fig. 3 The meiosis outcome of triploid Salvia miltiorrhiza A: Tetrad; B: Tetrad with micronuclei; C: Triad; D: Diad.

Fig. 4 Cross-section of anther of triploid Salvia miltiorrhiza Heliciform indicates abortive pollen.

Fig. 5 Pollen germination of triploid Salvia miltiorrhiza Very few pollen germinate (arrow).

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