Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (8): 1507-1514.DOI: 10.3969/j.issn.1000-2561.2019.08.008
• Phytophysiology and Biochemistry • Previous Articles Next Articles
XUE Xinxin,WU Xiaoping(),LUO Xuehua,WANG Wenbin,WANG Dapeng,ZHANG Yongfa,ZHAO Chunmei
Received:
2019-01-21
Revised:
2019-03-06
Online:
2019-08-25
Published:
2019-08-20
Contact:
WU Xiaoping
CLC Number:
XUE Xinxin,WU Xiaoping,LUO Xuehua,WANG Wenbin,WANG Dapeng,ZHANG Yongfa,ZHAO Chunmei. Effects of Potassium and Magnesium Deficiency on Leaf Physiological Characteristics and Chloroplast Ultrastructure of Anther Culture Seedling of Rubber Tree (Hevea brasiliensis)[J]. Chinese Journal of Tropical Crops, 2019, 40(8): 1507-1514.
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Fig. 2 Plant height and stem diameter of rubber tree seedling under different levels of potassium and magnesium deficiency Different lowercase letters above the bars indicate significant difference among treatments at P<0.05; * and ** indicate significant difference or extremely significant difference at 0.05 and 0.01 levels, respectively, “ns” indicates not significant difference.
Fig. 3 Photosynthetic pigment in leaves of rubber tree seedling under potassium and magnesium deficiency Different lowercase letters above the bars indicate significant difference among treatments at P<0.05; * and ** indicate significant difference or extremely significant difference at 0.05 and 0.01 levels, respectively, “ns” indicates not significant difference.
处理 Treatment | 钾含量 K concentration/% | 镁含量 Mg concentration/% | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
叶 leaf | 茎干 Stem | 茎皮 Stem bark | 叶柄 Petiole | 根 Root | 叶 leaf | 茎干 Stem | 茎皮 Stem bark | 叶柄 Petiole | 根 Root | |
-K-Mg | 1.020b | 0.911b | 0.991b | 1.451c | 1.140b | 0.190c | 0.104c | 0.195c | 0.158c | 0.075c |
-K | 1.081b | 0.874b | 1.076b | 1.606c | 1.135b | 0.264a | 0.249a | 0.470a | 0.311a | 0.144a |
-Mg | 1.582a | 1.530a | 1.605a | 3.023a | 1.526a | 0.179c | 0.100c | 0.179c | 0.142c | 0.058d |
CK | 1.558a | 1.364a | 1.640a | 2.742b | 1.506a | 0.231b | 0.155b | 0.321b | 0.251b | 0.107b |
P值(K) | 0.000** | 0.000** | 0.000** | 0.000** | 0.002** | 0.016* | 0.000** | 0.002** | 0.012** | 0.000** |
P值(Mg) | 0.783 | 0.171 | 0.420 | 0.256 | 0.885 | 0.000** | 0.000** | 0.000** | 0.000** | 0.000** |
P值(K×Mg) | 0.547 | 0.368 | 0.735 | 0.003** | 0.930 | 0.190 | 0.001** | 0.007** | 0.094 | 0.041* |
Tab. 1 Concentrations of potassium and magnesium and their variance analysis in different organs of rubber tree seedling under potassium and magnesium deficiency
处理 Treatment | 钾含量 K concentration/% | 镁含量 Mg concentration/% | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
叶 leaf | 茎干 Stem | 茎皮 Stem bark | 叶柄 Petiole | 根 Root | 叶 leaf | 茎干 Stem | 茎皮 Stem bark | 叶柄 Petiole | 根 Root | |
-K-Mg | 1.020b | 0.911b | 0.991b | 1.451c | 1.140b | 0.190c | 0.104c | 0.195c | 0.158c | 0.075c |
-K | 1.081b | 0.874b | 1.076b | 1.606c | 1.135b | 0.264a | 0.249a | 0.470a | 0.311a | 0.144a |
-Mg | 1.582a | 1.530a | 1.605a | 3.023a | 1.526a | 0.179c | 0.100c | 0.179c | 0.142c | 0.058d |
CK | 1.558a | 1.364a | 1.640a | 2.742b | 1.506a | 0.231b | 0.155b | 0.321b | 0.251b | 0.107b |
P值(K) | 0.000** | 0.000** | 0.000** | 0.000** | 0.002** | 0.016* | 0.000** | 0.002** | 0.012** | 0.000** |
P值(Mg) | 0.783 | 0.171 | 0.420 | 0.256 | 0.885 | 0.000** | 0.000** | 0.000** | 0.000** | 0.000** |
P值(K×Mg) | 0.547 | 0.368 | 0.735 | 0.003** | 0.930 | 0.190 | 0.001** | 0.007** | 0.094 | 0.041* |
处理 Treatment | 葡萄糖 Glucose/% | 蔗糖 Sucrose/% | 果糖 Fructose/% |
---|---|---|---|
-K-Mg | 0.77±0.01a | 0.057±0.003b | 1.21±0.07a |
-K | 0.62±0.04b | 0.044±0.003c | 0.90±0.03c |
-Mg | 0.57±0.05b | 0.064±0.001a | 1.10±0.04b |
CK | 0.46±0.05c | 0.049±0.002c | 0.61±0.05d |
P值(K) | 0.000** | 0.000** | 0.000** |
P值(Mg) | 0.001** | 0.000** | 0.000** |
P值(K×Mg) | 0.302ns | 0.580ns | 0.009** |
Tab. 2 Carbohydrates contents in leaves of rubber tree seedling and variance analysis under potassium and magnesium deficiency
处理 Treatment | 葡萄糖 Glucose/% | 蔗糖 Sucrose/% | 果糖 Fructose/% |
---|---|---|---|
-K-Mg | 0.77±0.01a | 0.057±0.003b | 1.21±0.07a |
-K | 0.62±0.04b | 0.044±0.003c | 0.90±0.03c |
-Mg | 0.57±0.05b | 0.064±0.001a | 1.10±0.04b |
CK | 0.46±0.05c | 0.049±0.002c | 0.61±0.05d |
P值(K) | 0.000** | 0.000** | 0.000** |
P值(Mg) | 0.001** | 0.000** | 0.000** |
P值(K×Mg) | 0.302ns | 0.580ns | 0.009** |
Fig. 4 Chloroplast ultrastructure in leaves of rubber tree seedling under different levels of potassium and magnesium deficiency A: -K-Mg; B: -K; C: -Mg; D: CK; Sg: Starch grannules; Pg: Plastoglobulus; Tl: Thylakoild lamella.
处理 Treatment | 叶绿体长度 Chloroplast length/μm | 叶绿体厚度 Chloroplast thickness/μm | 嗜饿体数 Plastoglobulus number | 淀粉粒数 Starch grannules number |
---|---|---|---|---|
-K-Mg | 4.36±0.23b | 2.38±0.05ab | 1.06±0.15b | 1.23±0.14a |
-K | 3.69±0.24c | 2.17±0.12b | 2.95±0.14a | 0.00±0.00c |
-Mg | 4.79±0.29b | 2.59±0.18a | 0.81±0.22b | 1.27±0.34a |
CK | 5.96±0.53a | 2.23±0.07b | 0.34±0.05c | 0.37±0.15b |
P值(K) | 0.000** | 0.069ns | 0.000** | 0.110ns |
P值(Mg) | 0.247ns | 0.003** | 0.000** | 0.000** |
P值(K×Mg) | 0.002** | 0.278ns | 0.000** | 0.201ns |
Tab. 3 Chloroplast structure in leaves of rubber tree seedling and variance analysis under different levels of potassium and magnesium deficiency
处理 Treatment | 叶绿体长度 Chloroplast length/μm | 叶绿体厚度 Chloroplast thickness/μm | 嗜饿体数 Plastoglobulus number | 淀粉粒数 Starch grannules number |
---|---|---|---|---|
-K-Mg | 4.36±0.23b | 2.38±0.05ab | 1.06±0.15b | 1.23±0.14a |
-K | 3.69±0.24c | 2.17±0.12b | 2.95±0.14a | 0.00±0.00c |
-Mg | 4.79±0.29b | 2.59±0.18a | 0.81±0.22b | 1.27±0.34a |
CK | 5.96±0.53a | 2.23±0.07b | 0.34±0.05c | 0.37±0.15b |
P值(K) | 0.000** | 0.069ns | 0.000** | 0.110ns |
P值(Mg) | 0.247ns | 0.003** | 0.000** | 0.000** |
P值(K×Mg) | 0.002** | 0.278ns | 0.000** | 0.201ns |
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