Determination of Oxine-Copper Residue in <i>Cucumis sativus</i> L. by Dispersive Solid Phase Extraction
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (7): 1449-1454.DOI: 10.3969/j.issn.1000-2561.2019.07.029

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Determination of Oxine-Copper Residue in Cucumis sativus L. by Dispersive Solid Phase Extraction

CHEN Siyu1,2,WANG Mingyue2,LIN Bing2,LV Daizhu2,*()   

  1. 1. College of Resources and Environment, Huazhong Agricultural University, Wuhan, Hubei 430070, China
    2. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received:2018-07-26 Revised:2018-12-20 Online:2019-07-25 Published:2019-08-01
  • Contact: LV Daizhu

Abstract:

A high performance liquid chromatography (HPLC) coupled with the dispersive solid phase extraction (d-SPE) method for the determination of oxine-copper residue in cucumber was established. The sample was extracted by acetonitrile and hydrochloric acid (0.1 mol/L). The extract was purified by C18. The analysis was carried out by phosphate buffer saline (PBS) and sodium dodecyl sulfate (SDS) -acetonitrile (65∶35) at a flow rate of 0.6 mL/min on SunFireC18 (4.6×150 mm, 5 μm) column, and detected by HPLC with UV detector at a ultraviolet absorption wavelength of 250 nm. The recovery was in 92.3%-107.7% with relative standard deviation (RSD) of 2.0%-7.7% at spiked levels of 0.02, 0.2 and 2 mg/kg. The method is simple, sensitive and accurate, and could be used in the determination of oxine-copper residue in cucumber.

Key words: Cucumis sativus L., oxine-copper, dispersive solid phase extraction, high performance liquid chromatography

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