Dissipation, Residues and Dietary Risk Assessment of Dinotefuran in Rice Ecosystem

doi:10.3969/j.issn.1000-2561.2019.07.027

Abstract

Abstract:

To evaluate the residue behavior and dietary intake risk of the dinotefuran in rice ecosystem, the residual trial of dinotefuran in rice was carried out in Hainan, Hunan and Heilongjiang in 2014. The method for the residue determination of dinotefuran in soil, paddy water, rice plant, brown rice, and rice husk was developed using high performance liquid chromatography with ultraviolet detector (HPLC-UV). The dietary risk assessment for China population based on dinotefuran residues data in rice was investigated. The samples were extracted by acetonitrile, purified by NH₂ column, detected by HPLC-UV. Results showed that the limit of detection (LOD) of dinotefuran was 0.08 ng. The limits of quantification (LOQ) of dinotefuran in rice plant, rice husk, brown rice, soil and paddy water was 0.1, 0.1, 0.05, 0.02, and 0.02 mg/kg, respectively. At fortified levels of 0.05 to 2 mg/kg, the average recoveries varied from 75% to 114% with the relative standard deviation of 0.5% to 19.0%. The dissipations of dinotefuran in rice plant, paddy water and soil were basically consistent with the first-order kinetic equation. The corresponding half-lives were about 0.5, 1, and 5 days, respectively. At the 14 ^th day after the last application, the terminal residues of dinotefuran in rice indicated that the supervised trials median residue (STMR) was 0.058 mg/kg, the highest residue (HR) was 0.13 mg/kg. STMR and HR were all below the Chinese MRL of 1 mg/kg. Risk assessment indicated a lower chronic risk after a long-term dietary intake of dinotefuran in rice for China population.

Key words: dinotefuran, rice, high performance liquid chromatography, residue, dissipation, risk assessment

CLC Number:

X839.2

GE Huilin,XIE Defang,SU Bingxia,LV Daizhu,ZHAO Fangfang. Dissipation, Residues and Dietary Risk Assessment of Dinotefuran in Rice Ecosystem[J]. Chinese Journal of Tropical Crops, 2019, 40(7): 1434-1440.

Figures/Tables 6

References 20

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样品 Sample	添加水平 Spiked level /(mg?kg^-1)	回收率 Recovery/%						相对标准偏差 RSD/%
样品 Sample	添加水平 Spiked level /(mg?kg^-1)	1	2	3	4	5	平均值 Average	相对标准偏差 RSD/%
稻株	0.10	80	80	90	90	80	84	6.5
	0.20	80	75	75	80	80	78	3.5
	0.50	80	82	82	84	82	82	1.7
土壤	0.02	90	80	75	75	75	79	8.3
	0.20	73	72	76	77	77	75	3.1
	0.50	70	89	77	70	75	76	10.3
田水	0.02	80	115	110	90	75	94	19.0
	0.20	94	96	95	96	96	95	0.9
	0.50	95	94	94	94	94	94	0.5
糙米	0.05	110	130	90	90	120	108	16.6
	0.20	95	95	95	100	95	96	2.3
	0.50	93	94	92	91	91	92	1.4
稻壳	0.10	120	100	110	120	120	114	7.8
	0.20	110	110	115	110	110	111	2.0
	0.50	92	94	94	94	94	94	1.0

样品 Sample	添加水平 Spiked level /(mg?kg^-1)	回收率 Recovery/%						相对标准偏差 RSD/%
样品 Sample	添加水平 Spiked level /(mg?kg^-1)	1	2	3	4	5	平均值 Average	相对标准偏差 RSD/%
稻株	0.10	80	80	90	90	80	84	6.5
	0.20	80	75	75	80	80	78	3.5
	0.50	80	82	82	84	82	82	1.7
土壤	0.02	90	80	75	75	75	79	8.3
	0.20	73	72	76	77	77	75	3.1
	0.50	70	89	77	70	75	76	10.3
田水	0.02	80	115	110	90	75	94	19.0
	0.20	94	96	95	96	96	95	0.9
	0.50	95	94	94	94	94	94	0.5
糙米	0.05	110	130	90	90	120	108	16.6
	0.20	95	95	95	100	95	96	2.3
	0.50	93	94	92	91	91	92	1.4
稻壳	0.10	120	100	110	120	120	114	7.8
	0.20	110	110	115	110	110	111	2.0
	0.50	92	94	94	94	94	94	1.0

样品 Sample	C₀/(mg?kg^-1)	K	R²	DT₅₀/d
海南稻株	6.85	2.168	0.993	0.32
湖南稻株	36.38	1.657	1.000	0.42
黑龙江稻株	37.56	1.406	0.994	0.49
海南土壤	0.062	0.133	0.917	5.21
湖南土壤	0.198	0.259	0.797	2.68
黑龙江土壤	0.518	0.134	0.972	5.17
海南田水	1.11	0.897	0.935	0.77
湖南田水	1.35	0.656	0.807	1.06
黑龙江田水	1.68	0.690	0.991	1.00

样品 Sample	C₀/(mg?kg^-1)	K	R²	DT₅₀/d
海南稻株	6.85	2.168	0.993	0.32
湖南稻株	36.38	1.657	1.000	0.42
黑龙江稻株	37.56	1.406	0.994	0.49
海南土壤	0.062	0.133	0.917	5.21
湖南土壤	0.198	0.259	0.797	2.68
黑龙江土壤	0.518	0.134	0.972	5.17
海南田水	1.11	0.897	0.935	0.77
湖南田水	1.35	0.656	0.807	1.06
黑龙江田水	1.68	0.690	0.991	1.00

试验点 Trial site	施药剂量 Application dosage/(g?hm^-2)	施药次数 Application frequency	采收间隔期 Preharvest interval/d	土壤Soil /(mg?kg^-1)	稻株Rice plant /(mg?kg^-1)	稻壳Rice husk /(mg?kg^-1)	糙米Brown rice /(mg?kg^-1)
海南	120	2	14	0.024	0.15	0.13	<0.05
	120	2	21	<0.02	<0.1	0.12	<0.05
	120	3	14	<0.02	0.11	0.13	<0.05
	120	3	21	0.022	<0.1	<0.1	<0.05
	180	2	14	<0.02	0.18	0.15	<0.05
	180	2	21	0.024	<0.1	<0.1	<0.05
	180	3	14	0.029	<0.1	0.15	<0.05
	180	3	21	0.032	<0.1	<0.1	<0.05
湖南	120	2	14	<0.02	0.21	0.95	0.07
	120	2	21	<0.02	<0.1	<0.1	<0.05
	120	3	14	<0.02	0.24	0.72	<0.05
	120	3	21	<0.02	<0.1	<0.1	<0.05
	180	2	14	<0.02	1.26	<0.1	<0.05
	180	2	21	<0.02	<0.1	1.54	0.07
	180	3	14	<0.02	1.4	2.49	0.13
	180	3	21	<0.02	<0.1	<0.1	<0.05
黑龙江	120	2	14	0.096	0.26	1.53	0.07
	120	2	21	0.14	0.27	0.79	0.065
	120	3	14	0.17	0.78	1.34	0.075
	120	3	21	0.13	0.66	0.86	0.07
	180	2	14	0.26	0.39	1.89	0.07
	180	2	21	0.25	0.37	1.3	0.07
	180	3	14	0.33	0.51	2.0	0.065
	180	3	21	0.3	0.32	2.15	0.085