Antioxidant and α-Glucosidase Inhibitory Activities of Different Polarity Fractions of the Extracts of the Young Leaves of Myrciaria cauliflora

doi:10.3969/j.issn.1000-2561.2019.07.018

Abstract

Abstract:

It is of great significance to search for natural antioxidants and α-glucosidase inhibitors from plants to diabetic patients. Based on the previous research, the polarity fractions of the ethanol extracts from the tender leaves of M. cauliflora were applied to determine the scavenging effects towards DPPH and ABTS ⁺ free radicals, inhibition on α-glucosidase activity, the contents of total polyphenols (TP) and total flavonoids (TF) by colorimetry, providing a reference evidence for the application of effective polarity fraction from the tender leaves of M. cauliflora in food, chemical and medical fields. The ethyl acetate fraction exhibited the highest contents of TP (33.92 mg/g) and TF (13.35 mg/g), followed by n-butanol fraction (TP 31.11 mg/g; TF 12.93 mg/g) and water fraction (TP 17.04 mg/g; TF 11.18 mg/g). TP and TF were not detected in the petroleum ether and dichloromethane fractions. The scavenging abilities of n-butanol fraction and ethyl acetate fraction towards DPPH and ABTS ⁺ radicals were higher than that of other fractions. The ethyl acetate fraction had the strongest inhibitory activity in the α-glucosidase, followed by the n-butanol and water fraction. This results indicated that the contents of TP and TF were higher in the ethyl acetate and n-butanol fractions, and the antioxidant activity and inhibition in α-glucosidase activity were stronger, compared with the other three polarity fractions, so the ethyl acetate and n-butanol fractions could be used as the main polar fractions to excavate active substances for antioxidation activity and inhibition activity in α-glucosidase.

Key words: Myrciaria cauliflora, young leaves, ethanol extracts, different polarity fractions, antioxidation, α-glucosidase

CLC Number:

S667.9

QIU Shanlian,LIN Baomei,ZHANG Shaoping,ZHANG Shuhe,ZHANG Shuai,HONG Jiamin,ZHENG Kaibin. Antioxidant and α-Glucosidase Inhibitory Activities of Different Polarity Fractions of the Extracts of the Young Leaves of Myrciaria cauliflora[J]. Chinese Journal of Tropical Crops, 2019, 40(7): 1373-1378.

Figures/Tables 5

References 26

[1]	邱珊莲, 林宝妹, 张少平 , 等. 嘉宝果果实不同部位营养成分分析[J]. 福建农业科技, 2018(1):1-3.
[2]	唐丽, 袁婷婷, 钟秋平 . 嘉宝果营养成分分析[J]. 经济林研究, 2014,32(2):120-124.
[3]	Wu S B, Long C L, Kennelly E J . Phytochemistry and health benefits of jaboticaba, an emerging fruit crop from Brazil[J]. Food Research International, 2013,54(1):148-159. DOI URL
[4]	Leite-legatti A V, Batista Â G, Dragano N R V , et al. Jaboticaba peel: Antioxidant compounds, antiproliferative and antimutagenic activities[J]. Food Research International, 2012,49(1):596-603. DOI URL
[5]	Borges L L, Conceição E C, Silveira D . Active compounds and medicinal properties of Myrciaria genus[J]. Food Chemistry, 2014,153:224-233. DOI URL
[6]	李慧敏, 刘艳妮, 王小蕊 , 等. 以α-葡萄糖苷酶活性抑制率为指标优化山茱萸果肉的提取条件[J]. 植物资源与环境学报, 2013,22(1):112-114.
[7]	Nishikawa T, Edelstein D, Du X L , et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage[J]. Nature, 2000,404(6779):787-790. DOI
[8]	Lhommeau I, Douillard S, Bigot E , et al. Serum resistance to singlet oxygen in patients with diabetes mellitus in comparison to healthy donors[J]. Metabolism, 2011,60(9):1340-l348. DOI URL
[9]	Hureau C, Faller P . Aβ-mediated ROS production by Cu ions: Structural insights, mechanisms and relevance to Alzheimer’s disease[J]. Biochimie, 2009,91(10):1212-1217. DOI URL
[10]	冯亚娟, 胡滨青, 周建华 . 甘草黄酮对糖尿病大鼠血糖、血脂水平及抗氧化能力的影响[J]. 山东医药, 2016,56(3):23-25.
[11]	金银萍, 李珊珊, 郑颖 , 等. 五味子抗糖尿病和抗氧化作用研究进展[J]. 特产研究, 2017,39(1):64-68.
[12]	邱珊莲, 林宝妹, 洪佳敏 , 等. 树葡萄植株不同部位醇提物抗氧化及抑制α-葡萄糖苷酶活性的比较研究[J]. 果树学报, 2018,35(3):311-318.
[13]	林宝妹, 郑开斌, 张帅 , 等. 不同成熟度树葡萄果实醇提物抗氧化及抑制α-葡萄糖苷酶活性研究[J]. 热带亚热带植物学报, 2018,26(3):233-240.
[14]	Li X C, Lin J, Gao Y X , et al. Antioxidant activity and mechanism of Rhizoma cimicifugae[J]. Chemistry Central Journal, 2012,6(1):140-150.
[15]	Hu Q P, Xu J G . Profiles of carotenoids, anthocyanins, phenolics, and antioxidant activity of selected color waxy corn grains during maturation[J]. Journal of Agricultural and Food Chemistry, 2011,59(5):2026-2033. DOI URL
[16]	梁丽, 李成江 . 2型糖尿病的抗氧化剂治疗[J]. 国外医学(老年医学分册), 2006,27(3):133-136.
[17]	张艳萍, 戴志远 . 山茱萸提取物不同极性部位体外抗氧化活性研究[J]. 中国粮油学报, 2009,24(3):98-102.
[18]	姚卫峰, 陈汀, 张丽 , 等. 女贞子醇提物不同极性部位的体外抗氧化活性研究[J]. 中国实验方剂学杂志, 2011,17(22):138-140.
[19]	张京芳, 王冬梅, 周丽 , 等. 香椿叶提取物不同极性部位体外抗氧化活性研究[J]. 中国食品学报, 2007,7(5):12-17.
[20]	吴峰华, 刘相真, 杨虎清 , 等. 覆盆子醇提物及其不同极性部位抗氧化活性研究[J]. 中国食品学报, 2012,12(2):24-29.
[21]	刘刚, 姜唯唯, 吴京 , 等. 芒果叶不同极性部位提取物的抗氧化活性[J]. 食品研究与开发, 2014,35(11):10-14.
[22]	孙霁寒, 黄玲艳, 顾嘉昌 , 等. 豆腐柴提取物不同极性部位的总酚、总黄酮含量及体外抗氧化活性[J]. 食品工业科技, 2017,38(15):55-58.
[23]	薛雅荣, 薛海波, 郝丽琴 , 等. 菠萝皮渣醇提物不同极性部位抗氧化活性研究[J]. 食品研究与开发, 2016,37(20):8-11.
[24]	张燕, 李琳琳 , 木合布力·阿布力孜, 等. 新疆昆仑雪菊5种提取物对α-葡萄糖昔酶活性的影响[J]. 中国实验方剂学杂志, 2011,17(7):166-169.
[25]	胡鑫, 齐艳菲, 王兴云 , 等. 8种傣药提取物体外抑制α-糖苷酶活性的研究[J]. 中南民族大学学报(自然科学版), 2013,32(3):60-62, 117.
[26]	许有瑞 . 甘草中α-葡萄糖苷酶抑制剂的筛选研究[D]. 兰州: 兰州大学, 2006.

样品 Samples	总多酚含量 Content of total polyphenols/ (mg·g^-1 )	总黄酮含量 Content of total flavonoid/ (mg·g^-1 )
粗提液 Crude extract	91.09±0.41^a	41.10±0.42^a
石油醚相 Petrol ether phase	—	—
二氯甲烷相Dichloromethane phase	—	—
乙酸乙酯相Ethyl acetate phase	33.92±0.43^b	13.35±0.27^b
正丁醇相N-butanol phase	31.11±0.25^c	12.93±0.35^b
水相Water phase	17.04±0.46^d	11.18±0.59^c

样品 Samples	总多酚含量 Content of total polyphenols/ (mg·g^-1 )	总黄酮含量 Content of total flavonoid/ (mg·g^-1 )
粗提液 Crude extract	91.09±0.41^a	41.10±0.42^a
石油醚相 Petrol ether phase	—	—
二氯甲烷相Dichloromethane phase	—	—
乙酸乙酯相Ethyl acetate phase	33.92±0.43^b	13.35±0.27^b
正丁醇相N-butanol phase	31.11±0.25^c	12.93±0.35^b
水相Water phase	17.04±0.46^d	11.18±0.59^c

样品 Samples	回归方程 Regression equation	R²	IC₅₀ /(g·L^-1)
粗提液 Crude extract	y=110.9054x+5.2374	0.9604	0.4036
石油醚相 Petrol ether phase	—	—	—
二氯甲烷相 Dichloromethane phase	—	—	—
乙酸乙酯相 Ethyl acetate phase	y=45.1889x+6.2520	0.9856	0.9681
正丁醇相 N-butanol phase	y=43.3100x+8.9707	0.9940	0.9473
水相 Water phase	y=25.3556x-1.6366	0.9576	2.0365
抗坏血酸 Ascorbic acid	y=854.7750x-0.7475	0.9909	0.0594

样品 Samples	回归方程 Regression equation	R²	IC₅₀ /(g·L^-1)
粗提液 Crude extract	y=110.9054x+5.2374	0.9604	0.4036
石油醚相 Petrol ether phase	—	—	—
二氯甲烷相 Dichloromethane phase	—	—	—
乙酸乙酯相 Ethyl acetate phase	y=45.1889x+6.2520	0.9856	0.9681
正丁醇相 N-butanol phase	y=43.3100x+8.9707	0.9940	0.9473
水相 Water phase	y=25.3556x-1.6366	0.9576	2.0365
抗坏血酸 Ascorbic acid	y=854.7750x-0.7475	0.9909	0.0594

样品 Samples	回归方程 Regression equation	R²	IC₅₀/(g·L^-1)
粗提液 Crude extract	y=100.7607x+9.1715	0.9986	0.4052
石油醚相 Petrol ether phase	y=1.7543x+6.6634	0.9935	24.7031
二氯甲烷相 Dichloromethane phase	—	—	—
乙酸乙酯相 Ethyl acetate phase	y=39.0584x+8.2856	0.9942	1.0680
正丁醇相 N-butanol phase	y=37.1979x+5.0920	0.9924	1.2073
水相 Water phase	y=15.3430x+7.9746	0.9901	2.7508
抗坏血酸 Ascorbic acid	y=623.7699x-1.5367	0.9994	0.0826