Change of Root Growth of Muskmelon,Soil Biological Properties and Bacterial Diversity in Rhizosphere under Wood Vinegar Application

doi:10.3969/j.issn.1000-2561.2019.07.003

Abstract

Abstract:

To develop a new liquid fertilizer and soil conditioner with wood vinegar, the effects of wood vinegar on the root growth, soil biological properties and soil bacterial diversity in the rhizosphere of muskmelon were conducted with pot culture using traditional and modern analyzed techniques. The results showed that the root growth, soil enzyme activities and microbial biomass (C, N, P) were not only significantly improved, the richness and diversity indexes, which including Chao1and Shannon, were also increased after using 600 times dilution of wood vinegar. Proteobacteria, Actinobacteria, Acidobacteria, Verrucomicrobia and Chloroflexi were the most abundant under phyla level, and Subgroup 6_no rank, Saccharibacteria, Gemmatimonadaceae, Sphingomonas, Soil Crenarchaeotic Group, Bacillus, Rhodospirillaceae, Roseiflexus, Lactococcus and Acidimicrobiales were the dominant genus under genus level both in CK and wood vinegar treatments. It meant that the soil bacterial community was not changed using different dilutions of wood vinegar. Moreover, higher diversity of bacteria, rapid growth of root and more favorable soil biological properties in the rhizosphere of muskmelon by fertilizing with 600 times dilution of wood vinegar.

Key words: wood vinegar, muskmelon, root, bacterial diversity, high-throughput sequencing

CLC Number:

S652

ZHANG Chuanjin,WU Renmin,WANG Shuaishuai,ZHOU Liuqiang,YANG Shangdong,TAN Hongwei. Change of Root Growth of Muskmelon,Soil Biological Properties and Bacterial Diversity in Rhizosphere under Wood Vinegar Application[J]. Chinese Journal of Tropical Crops, 2019, 40(7): 1265-1271.

Figures/Tables 9

References 27

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处理 Treatment	总根长 Length/cm	总根表面积 Surf area/cm²	总根体积 Root volume/cm³	根尖数 Tips
CK	1274.6±32.7^c	259.6±12.5^d	2.93±0.42^c	6237±235^c
A	1569.4±48.1^b	301.5±6.7^b	3.41±0.33^b	8492±362^b
B	1891.7±54.7^a	352.8±9.1^a	4.22±0.64^a	10324±458^a
C	1303.3±28.9^c	261.2±10.7^c	3.06±0.28^c	5873±413^d

处理 Treatment	总根长 Length/cm	总根表面积 Surf area/cm²	总根体积 Root volume/cm³	根尖数 Tips
CK	1274.6±32.7^c	259.6±12.5^d	2.93±0.42^c	6237±235^c
A	1569.4±48.1^b	301.5±6.7^b	3.41±0.33^b	8492±362^b
B	1891.7±54.7^a	352.8±9.1^a	4.22±0.64^a	10324±458^a
C	1303.3±28.9^c	261.2±10.7^c	3.06±0.28^c	5873±413^d

处理 Treatment	细菌 Bacteria/ （10⁶CFU·g^-1）	真菌 Fungi/ （10⁴ CFU·g^-1）	放线菌 Actinomycetes/（10⁶ CFU·g^-1）
CK	20.11±0.41^c	4.07±0.14^c	19.32±0.69^c
A	26.58±0.22^b	5.96±0.27^b	26.59±1.32^b
B	39.24±0.36^a	7.32±0.31^a	34.91±0.97^a
C	21.63±0.19^c	4.11±0.44^c	20.17±1.34^c

处理 Treatment	细菌 Bacteria/ （10⁶CFU·g^-1）	真菌 Fungi/ （10⁴ CFU·g^-1）	放线菌 Actinomycetes/（10⁶ CFU·g^-1）
CK	20.11±0.41^c	4.07±0.14^c	19.32±0.69^c
A	26.58±0.22^b	5.96±0.27^b	26.59±1.32^b
B	39.24±0.36^a	7.32±0.31^a	34.91±0.97^a
C	21.63±0.19^c	4.11±0.44^c	20.17±1.34^c

处理 Treatment	生物量碳 Microbial biomass C	生物量氮 Microbial biomass N	生物量磷 Microbial biomass P
CK	112.92±2.17^c	14.79±1.12^d	18.53±1.41^c
A	136.69±3.06^b	18.08±1.36^b	22.50±1.12^b
B	165.40±1.90^a	20.82±1.47^a	24.49±1.07^a
C	115.35±2.70^c	16.96±1.03^c	19.15±1.22^c