Function of Red Spider Mite (Tetranychus cinnabarinus) Transcription Factor TcNrf2 on Regulating the Transcription of Antioxidant Enzyme Genes

doi:10.3969/j.issn.1000-2561.2019.06.018

Abstract

Abstract:

The transcription factor Nrf2 plays a key role in the regulation of antioxidant enzymes in model organisms, but up to now, there was a lack of study indicating that Nrf2 can regulate antioxidant enzymes in mites. In this study, the Nrf2 inhibitors and activators were used to treat with the red spider mite (Tetranychus cinnabarinus). The transcription of TcSOD and TcCAT were studied by inhibiting and activating the transcription factor TcNrf2. The results showed that the transcriptions of TcNrf2, TcSOD and TcCAT decreased significantly after feeding on mite-resistant and mite-susceptible cassava cultivars for 1, 4 and 8 d, while were not affected by retinoic acid treatment. However, the transcription of those genes significantly increased after treated by TBHQ (tert-butylhydroquinone) and D3T (1,3-dithiole-2-thione-4,5-dithiolat), respectively, suggesting that the transcriptions of TcNrf2 and its regulated TcSOD and TcCAT genes were consistent. This study directly proves that TcNrf2 gene plays an important role in cassava mite resistance at the molecular level, which would lay a theoretical foundation for the molecular mechanism of mite resistance to cassava.

Key words: cassava, Tetranychus cinnabarinus, transcription factor TcNrf2, regulation function

CLC Number:

Gao Jintao,LIANG Xiao,WU Chunling,CHEN Qing,CHEN Qian. Function of Red Spider Mite (Tetranychus cinnabarinus) Transcription Factor TcNrf2 on Regulating the Transcription of Antioxidant Enzyme Genes[J]. Chinese Journal of Tropical Crops, 2019, 40(6): 1163-1170.

Figures/Tables 9

References 24

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引物名称 Primers	序列 Sequences (5'-3')	退火温度 T_m /℃	产物大小 Product size/bp
TcNrf2-F	GAGGGGATCTGCTTGTCCAG	59.8	111
TcNrf2-R	GCTATTGTGAGCGATTGGCG	60.0	111
TcSOD-F	TGGTGACAACACAAACGG	58.3	196
TcSOD-R	CTCCACAAGCAATACGGC	58.4	196
TcCAT-F	TACAAACGGTGGGCAAGA	59.6	212
TcCAT-R	GAGGGTCACGAACAGTATCAGC	60.4	212
Tcactin-F	CAGCCATGTATGTTGCCATC	59.4	166
Tcactin-R	AAATCACGACCAGCCAAATC	58.5	166

引物名称 Primers	序列 Sequences (5'-3')	退火温度 T_m /℃	产物大小 Product size/bp
TcNrf2-F	GAGGGGATCTGCTTGTCCAG	59.8	111
TcNrf2-R	GCTATTGTGAGCGATTGGCG	60.0	111
TcSOD-F	TGGTGACAACACAAACGG	58.3	196
TcSOD-R	CTCCACAAGCAATACGGC	58.4	196
TcCAT-F	TACAAACGGTGGGCAAGA	59.6	212
TcCAT-R	GAGGGTCACGAACAGTATCAGC	60.4	212
Tcactin-F	CAGCCATGTATGTTGCCATC	59.4	166
Tcactin-R	AAATCACGACCAGCCAAATC	58.5	166

木薯品种 Cassava cultivars	观察时间 Time/d	BA浓度Concentration of BA/(μmol·L^-1)
木薯品种 Cassava cultivars	观察时间 Time/d	20.00	10.00	5.00	2.50	1.25	0 (control)
BRA900	1	6.67±1.92^a	6.67±1.92^a	3.33±0.56^b	3.33±0.00^b	3.33±0.56^b	0.00^c
	4	14.29±2.13^a	10.34±1.86^bc	7.14±1.35^c	4.17±1.82^d	3.45±1.34^d	0.00^e
	8	20.96±4.05^a	17.01±3.78^b	10.47±1.91^c	7.50±1.82^d	6.78±1.90^e	0.00^f
C1115	1	13.33±2.18^a	10.00±1.84^b	6.67±1.57^c	3.33±0.00^d	3.33±0.56^d	3.33±0.56^d
C1115	4	21.43±3.15^a	13.04±2.78^b	11.58±3.28^b	12.05±1.76^b	12.14±1.87^b	12.57±2.16^b
	8	64.76±5.33^a	53.04±4.62^b	47.25±4.85^c	45.33±1.76^c	39.14±1.87^c	32.57±2.16^d

木薯品种 Cassava cultivars	观察时间 Time/d	BA浓度Concentration of BA/(μmol·L^-1)
木薯品种 Cassava cultivars	观察时间 Time/d	20.00	10.00	5.00	2.50	1.25	0 (control)
BRA900	1	6.67±1.92^a	6.67±1.92^a	3.33±0.56^b	3.33±0.00^b	3.33±0.56^b	0.00^c
	4	14.29±2.13^a	10.34±1.86^bc	7.14±1.35^c	4.17±1.82^d	3.45±1.34^d	0.00^e
	8	20.96±4.05^a	17.01±3.78^b	10.47±1.91^c	7.50±1.82^d	6.78±1.90^e	0.00^f
C1115	1	13.33±2.18^a	10.00±1.84^b	6.67±1.57^c	3.33±0.00^d	3.33±0.56^d	3.33±0.56^d
C1115	4	21.43±3.15^a	13.04±2.78^b	11.58±3.28^b	12.05±1.76^b	12.14±1.87^b	12.57±2.16^b
	8	64.76±5.33^a	53.04±4.62^b	47.25±4.85^c	45.33±1.76^c	39.14±1.87^c	32.57±2.16^d

木薯品种 Cassava cultivars	观察时间 Time/d	RA浓度Concentration of RA/(μmol·L^-1)
木薯品种 Cassava cultivars	观察时间 Time/d	20.00	10.00	5.00	2.50	1.25	0 (control)
BRA900	1	3.33±1.92^a	0. 00^b	0.00^b	0.00^b	0.00^b	0.00^b
	4	4.71±1.13^b	7.14±1.86^a	7.57±1.35^a	3.33±1.92^c	0.00^d	0.00^d
	8	9.85±2.05^a	8.14±1.86^a	8.57±1.35^a	8.33±1.74^a	3.15±0.97^b	3.56±0.53^b
C1115	1	3.33±1.92^a	3.33±1.92^a	0.00^b	0.00^b	0.00^b	0.00^b
C1115	4	18.71±3.15^a	17.14±2.78^a	16.57±3.28^a	15.25±2.14^a	15.26±1.37^a	15.33±1.92^a
	8	38.71±7.23^a	36.14±5.79^a	36.57±6.28^a	34.28±5.18^a	33.76±6.31^a	34.57±5.73^a