Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (6): 1163-1170.DOI: 10.3969/j.issn.1000-2561.2019.06.018

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Function of Red Spider Mite (Tetranychus cinnabarinus) Transcription Factor TcNrf2 on Regulating the Transcription of Antioxidant Enzyme Genes

Gao Jintao1,2,LIANG Xiao2,WU Chunling2,CHEN Qing2,*(),CHEN Qian1,2   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2018-10-28 Revised:2019-03-04 Online:2019-06-25 Published:2019-07-02
  • Contact: CHEN Qing

Abstract:

The transcription factor Nrf2 plays a key role in the regulation of antioxidant enzymes in model organisms, but up to now, there was a lack of study indicating that Nrf2 can regulate antioxidant enzymes in mites. In this study, the Nrf2 inhibitors and activators were used to treat with the red spider mite (Tetranychus cinnabarinus). The transcription of TcSOD and TcCAT were studied by inhibiting and activating the transcription factor TcNrf2. The results showed that the transcriptions of TcNrf2, TcSOD and TcCAT decreased significantly after feeding on mite-resistant and mite-susceptible cassava cultivars for 1, 4 and 8 d, while were not affected by retinoic acid treatment. However, the transcription of those genes significantly increased after treated by TBHQ (tert-butylhydroquinone) and D3T (1,3-dithiole-2-thione-4,5-dithiolat), respectively, suggesting that the transcriptions of TcNrf2 and its regulated TcSOD and TcCAT genes were consistent. This study directly proves that TcNrf2 gene plays an important role in cassava mite resistance at the molecular level, which would lay a theoretical foundation for the molecular mechanism of mite resistance to cassava.

Key words: cassava, Tetranychus cinnabarinus, transcription factor TcNrf2, regulation function

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