Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (5): 995-1001.DOI: 10.3969/j.issn.1000-2561.2019.05.023

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Optimization of Liquid Fermentation Conditions of Bacillus subtilis CS27

ZHANG Hui,LIN Chenqiang,WU Dahua,CHEN Jichen,CAI Haisong()   

  1. Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
  • Received:2018-11-08 Revised:2019-01-10 Online:2019-05-25 Published:2019-05-31
  • Contact: Haisong CAI


The fermentation medium and culture conditions were optimized by single factor and orthogonal experiments. The optimum fermentation medium was 1.5% soybean powder, 0.5% molasses, 0.8% ammonium chloride, 1.0% NaCl, 0.1% sodium citrate, 0.5% calcium carbonate, 0.1% MgSO4·7H2O. The optimum fermentation conditions were fermentation temperature 32 ℃, liquid volume 50 mL / 250 mL, inoculation amount 10%, initial pH value 7.0. The cell biomass could reach 1.75 × 10 9cfu/mL under the optimized fermentation medium, which could provide a scientific basis for the large scale production and application of B. subtilis CS27.

Key words: Bacillus subtilis, fermentation conditions optimization, microbial fungicide, carbon source, nitrogen source, inorganic salt

CLC Number: