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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (5): 995-1001.DOI: 10.3969/j.issn.1000-2561.2019.05.023
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ZHANG Hui,LIN Chenqiang,WU Dahua,CHEN Jichen,CAI Haisong(
)
Received:2018-11-08
Revised:2019-01-10
Online:2019-05-25
Published:2019-05-31
Contact:
Haisong CAI
CLC Number:
ZHANG Hui,LIN Chenqiang,WU Dahua,CHEN Jichen,CAI Haisong. Optimization of Liquid Fermentation Conditions of Bacillus subtilis CS27[J]. Chinese Journal of Tropical Crops, 2019, 40(5): 995-1001.
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| 碳源种类 Carbon source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) | 有机氮源种类 Organic nitrogen source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) | 无机氮源种类 Inorganic nitrogen source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) |
|---|---|---|---|---|---|
| 麦芽糖 | 4.40cBC | 大豆蛋白胨 | 11.3bBC | 氯化铵 | 18.00aA |
| 蔗糖 | 7.10bB | 蛋白胨 | 0.600dD | 硫酸铵 | 14.50bB |
| 玉米淀粉 | 6.90bB | 黄豆粉 | 20.3aA | 草酸铵 | 6.50cC |
| 葡萄糖 | 3.80cBC | 豆粕 | 14.5bB | 脲 | 4.27dD |
| 玉米粉 | 6.80bB | 酵母粉 | 12.5bB | 硝酸铵 | 0.367eE |
| 糖蜜 | 11.9aA | 牛肉膏 | 7.50cC | 硝酸钾 | 0.100eE |
| CK1 | 2.40cC | CK2 | 2.90dD | CK3 | 14.10bB |
Tab. 1 Cell biomass of CS27 strain with different C and N sources
| 碳源种类 Carbon source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) | 有机氮源种类 Organic nitrogen source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) | 无机氮源种类 Inorganic nitrogen source | 细胞生物量 Cell biomass/ (×108 cfu?g-1) |
|---|---|---|---|---|---|
| 麦芽糖 | 4.40cBC | 大豆蛋白胨 | 11.3bBC | 氯化铵 | 18.00aA |
| 蔗糖 | 7.10bB | 蛋白胨 | 0.600dD | 硫酸铵 | 14.50bB |
| 玉米淀粉 | 6.90bB | 黄豆粉 | 20.3aA | 草酸铵 | 6.50cC |
| 葡萄糖 | 3.80cBC | 豆粕 | 14.5bB | 脲 | 4.27dD |
| 玉米粉 | 6.80bB | 酵母粉 | 12.5bB | 硝酸铵 | 0.367eE |
| 糖蜜 | 11.9aA | 牛肉膏 | 7.50cC | 硝酸钾 | 0.100eE |
| CK1 | 2.40cC | CK2 | 2.90dD | CK3 | 14.10bB |
Fig. 1 The cell biomass of CS27 strain cultured at different concentrations of molasses
Fig. 2 The cell biomass of CS27 strain cultured at different concentrations of soybean powder
Fig. 3 The cell biomass of CS27 strain at different ammoniumchloride concentrations
| 试验号 No. | 因素 Factors | 细胞生物量 Cell biomass /(×108cfu?g-1) | ||
|---|---|---|---|---|
| (A)黄豆粉 Soybean powder/% | (B)糖蜜 Molasses /% | (C)氯化铵 Ammonium chloride /% | ||
| 1 | 1(1.0) | 1(0.5) | 1(0.3) | 8.40 |
| 2 | 1(1.0) | 2(1.0) | 2(0.5) | 10.10 |
| 3 | 1(1.0) | 3(1.5) | 3(0.8) | 5.40 |
| 4 | 2(1.5) | 1(0.5) | 3(0.8) | 12.90 |
| 5 | 2(1.5) | 2(1.0) | 1(0.3) | 12.30 |
| 6 | 2(1.5) | 3(1.5) | 2(0.5) | 1.51 |
| 7 | 3(2.0) | 1(0.5) | 2(0.5) | 6.80 |
| 8 | 3(2.0) | 2(1.0) | 3(0.8) | 5.90 |
| 9 | 3(2.0) | 3(1.5) | 1(0.3) | 9.20 |
| K1 | 2.39×109 | 2.81×109 | 2.99×109 | |
| K2 | 2.67×109 | 2.83×109 | 1.84×109 | |
| K3 | 2.19×109 | 1.61×109 | 2.42×109 | |
| R | 2.14×109 | 1.70×108 | 7.40×108 | |
Tab. 2 L9(33) orthogonal experimental design and results analysis
| 试验号 No. | 因素 Factors | 细胞生物量 Cell biomass /(×108cfu?g-1) | ||
|---|---|---|---|---|
| (A)黄豆粉 Soybean powder/% | (B)糖蜜 Molasses /% | (C)氯化铵 Ammonium chloride /% | ||
| 1 | 1(1.0) | 1(0.5) | 1(0.3) | 8.40 |
| 2 | 1(1.0) | 2(1.0) | 2(0.5) | 10.10 |
| 3 | 1(1.0) | 3(1.5) | 3(0.8) | 5.40 |
| 4 | 2(1.5) | 1(0.5) | 3(0.8) | 12.90 |
| 5 | 2(1.5) | 2(1.0) | 1(0.3) | 12.30 |
| 6 | 2(1.5) | 3(1.5) | 2(0.5) | 1.51 |
| 7 | 3(2.0) | 1(0.5) | 2(0.5) | 6.80 |
| 8 | 3(2.0) | 2(1.0) | 3(0.8) | 5.90 |
| 9 | 3(2.0) | 3(1.5) | 1(0.3) | 9.20 |
| K1 | 2.39×109 | 2.81×109 | 2.99×109 | |
| K2 | 2.67×109 | 2.83×109 | 1.84×109 | |
| K3 | 2.19×109 | 1.61×109 | 2.42×109 | |
| R | 2.14×109 | 1.70×108 | 7.40×108 | |
| 无机盐种类 Inorganic salt | 细胞生物量 Cell biomass /(×108 cfu?g-1) |
|---|---|
| 柠檬酸钠 | 19.60aA |
| MgSO4·7H2O | 17.60bB |
| 碳酸钙 | 15.90cBC |
| CK | 14.10dCD |
| FeSO4·7H2O | 12.80dD |
| 氯化钙 | 9.80eE |
| ZnSO4·7H2O | 9.53eE |
| 磷酸氢二钾 | 5.43fF |
| 磷酸二氢钾 | 2.80gG |
Tab. 3 The cell biomass of CS27 strain with different inorganic salt
| 无机盐种类 Inorganic salt | 细胞生物量 Cell biomass /(×108 cfu?g-1) |
|---|---|
| 柠檬酸钠 | 19.60aA |
| MgSO4·7H2O | 17.60bB |
| 碳酸钙 | 15.90cBC |
| CK | 14.10dCD |
| FeSO4·7H2O | 12.80dD |
| 氯化钙 | 9.80eE |
| ZnSO4·7H2O | 9.53eE |
| 磷酸氢二钾 | 5.43fF |
| 磷酸二氢钾 | 2.80gG |
Fig. 4 The cell biomass of CS27 strain cultured at different concentrations of sodium chloride
Fig. 5 The cell biomass of CS27 strain cultured at different concentrations of magnesium sulfate
Fig. 6 The cell biomass of CS27 strain cultured at different concentrations of sodium citrate
Fig. 7 The cell biomass of CS27 strain cultured at different Concentrations of calcium carbonate
| 试验号 No. | 因素 Factors | 细胞生量 Cell biomass /(×108 cfu?g-1) | |||
|---|---|---|---|---|---|
| （A'）柠檬酸钠 Sodium citrate/% | （B'）七水合硫酸镁 Magnesium sulfate/% | （C'）碳酸钙 Calcium carbonate /% | （D'）氯化钠 Sodium chloride /% | ||
| 1 | 1(0.05) | 1(0.1) | 1(0.2) | 1(0.6) | 12.60 |
| 2 | 1(0.05) | 2(0.3) | 2(0.5) | 2(0.8) | 7.00 |
| 3 | 1(0.05) | 3(0.5) | 3(0.8) | 3(1.0) | 6.90 |
| 4 | 2(0.10) | 1(0.1) | 2(0.5) | 3(1.0) | 22.90 |
| 5 | 2(0.10) | 2(0.3) | 3(0.8) | 1(0.6) | 9.60 |
| 6 | 2(0.10) | 3(0.5) | 1(0.2) | 2(0.8) | 7.30 |
| 7 | 3(0.15) | 1(0.1) | 3(0.8) | 2(0.8) | 14.00 |
| 8 | 3(0.15) | 2(0.3) | 1(0.2) | 3(1.0) | 12.40 |
| 9 | 3(0.15) | 3(0.5) | 2(0.5) | 1(0.6) | 11.40 |
| K1 | 26.50 | 49.50 | 32.30 | 33.60 | |
| K2 | 39.80 | 29.00 | 41.30 | 28.30 | |
| K3 | 37.80 | 25.60 | 30.50 | 42.20 | |
| R | 13.30 | 20.50 | 9.00 | 8.80 | |
Tab. 4 L9(33) Orthogonal experimental design and results analysis
| 试验号 No. | 因素 Factors | 细胞生量 Cell biomass /(×108 cfu?g-1) | |||
|---|---|---|---|---|---|
| （A'）柠檬酸钠 Sodium citrate/% | （B'）七水合硫酸镁 Magnesium sulfate/% | （C'）碳酸钙 Calcium carbonate /% | （D'）氯化钠 Sodium chloride /% | ||
| 1 | 1(0.05) | 1(0.1) | 1(0.2) | 1(0.6) | 12.60 |
| 2 | 1(0.05) | 2(0.3) | 2(0.5) | 2(0.8) | 7.00 |
| 3 | 1(0.05) | 3(0.5) | 3(0.8) | 3(1.0) | 6.90 |
| 4 | 2(0.10) | 1(0.1) | 2(0.5) | 3(1.0) | 22.90 |
| 5 | 2(0.10) | 2(0.3) | 3(0.8) | 1(0.6) | 9.60 |
| 6 | 2(0.10) | 3(0.5) | 1(0.2) | 2(0.8) | 7.30 |
| 7 | 3(0.15) | 1(0.1) | 3(0.8) | 2(0.8) | 14.00 |
| 8 | 3(0.15) | 2(0.3) | 1(0.2) | 3(1.0) | 12.40 |
| 9 | 3(0.15) | 3(0.5) | 2(0.5) | 1(0.6) | 11.40 |
| K1 | 26.50 | 49.50 | 32.30 | 33.60 | |
| K2 | 39.80 | 29.00 | 41.30 | 28.30 | |
| K3 | 37.80 | 25.60 | 30.50 | 42.20 | |
| R | 13.30 | 20.50 | 9.00 | 8.80 | |
Fig. 8 Effect of temperature on cell biomass of CS27 strain
Fig. 9 Effect of initial pH on cell biomass of CS27 strain
Fig. 10 Effect of liquid volume in flask on cell biomass of CS27 strain
Fig. 11 Effect of inoculum concentration on cell biomass of CS27 strain
Fig. 12 Effect of the mesh of soybean powder on cell biomass of CS27 strain
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