Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (5): 939-946.DOI: 10.3969/j.issn.1000-2561.2019.05.015

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Proteomic Analysis of Different Sugarcane Genotypes in Response to Pokkah Boeng Disease

WANG Zeping,LIN Shanhai,LIANG Qiang,LI Changning,SONG Xiupeng,LIU Lu,LI Yijie()   

  1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture & Rural Affairs, Nanning, Guangxi 530007, China
  • Received:2018-07-02 Revised:2019-02-27 Online:2019-05-25 Published:2019-05-31
  • Contact: Yijie LI


This study was to provide a theoretical guidance and scientific reference for sugarcane resistance breeding and disease prevention by analyzing the physiological and biochemical mechanism in the infection process of sugarcane response to pokkah boeng disease. With spore suspension of Fusarium verticillioides (the mainly pathogenic fungus of pokkah boeng disease in China) as the pathogeny and YT94/128 (HR), GT37 (HS) as the tested materials by syringe inoculation under a greenhouse, and the samples were collected at the 14 th post-inoculation with the highest disease index to carry out quantitative expression analysis by isobaric tags for relative and absolute quantitation (iTRAQ). The results showed a total of 3707 proteins were successfully identified from GT37, and 542 differentially expressed proteins (DEPs) were obtained, of which 187 were up-regulated and 355 were down-regulated. Meanwhile, a total of 3068 proteins were successfully identified from YT94/128, and 449 DEPs were obtained, of which 191 were up-regulated and 258 were down-regulated. In a word, there are significant differences in the protein composition of sugarcane varieties with different genetic backgrounds, and it is speculated that this is the important molecular basis for the difference of resistance on pokkah boeng disease between different sugarcane varieties.

Key words: sugarcane, pokkah boeng disease, Fusarium verticillioides, proteomic, infection

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