Proteomic Analysis of Different Sugarcane Genotypes in Response to Pokkah Boeng Disease

doi:10.3969/j.issn.1000-2561.2019.05.015

Abstract

Abstract:

This study was to provide a theoretical guidance and scientific reference for sugarcane resistance breeding and disease prevention by analyzing the physiological and biochemical mechanism in the infection process of sugarcane response to pokkah boeng disease. With spore suspension of Fusarium verticillioides (the mainly pathogenic fungus of pokkah boeng disease in China) as the pathogeny and YT94/128 (HR), GT37 (HS) as the tested materials by syringe inoculation under a greenhouse, and the samples were collected at the 14 ^th post-inoculation with the highest disease index to carry out quantitative expression analysis by isobaric tags for relative and absolute quantitation (iTRAQ). The results showed a total of 3707 proteins were successfully identified from GT37, and 542 differentially expressed proteins (DEPs) were obtained, of which 187 were up-regulated and 355 were down-regulated. Meanwhile, a total of 3068 proteins were successfully identified from YT94/128, and 449 DEPs were obtained, of which 191 were up-regulated and 258 were down-regulated. In a word, there are significant differences in the protein composition of sugarcane varieties with different genetic backgrounds, and it is speculated that this is the important molecular basis for the difference of resistance on pokkah boeng disease between different sugarcane varieties.

Key words: sugarcane, pokkah boeng disease, Fusarium verticillioides, proteomic, infection

CLC Number:

S566.1

WANG Zeping,LIN Shanhai,LIANG Qiang,LI Changning,SONG Xiupeng,LIU Lu,LI Yijie. Proteomic Analysis of Different Sugarcane Genotypes in Response to Pokkah Boeng Disease[J]. Chinese Journal of Tropical Crops, 2019, 40(5): 939-946.

Figures/Tables 4

References 25

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登录号 UniProtKB Entry	基因名称 Gene Name	蛋白名称 Protein name			抗病（接种）与抗病（对照） R(JZ) vs. R(CK)	感病（接种）与感病（对照） S(JZ) vs. S(CK)
光合作用 Photosynthesis
A1E9R9	AtpH	ATP synthase CF0 C subunit			1.39	-1.13
C5YVW5	Lhcb1	Chlorophyll a-b binding protein			-1.88	-1.65
C5WTC1	Lhcb2	Chlorophyll a-b binding protein			-1.44	-1.38
C5X7M3	Lhcb1	Chlorophyll a-b binding protein			-1.54	-1.26
A1E9U0	PsbF	Cytochrome b6-f complex iron-sulfur subunit			1.54	1.13
C5XL99	PetB	Cytochrome b6-f complex iron-sulfur subunit			1.77	1.28
C5WQF7	PetF	Photosynthetic election transport			-1.38	-1.24
C5YLG2	PetF	Photosynthetic election transport			2.25	1.88
C5X3S4	PsaG	Photosystem Ⅰ subunit Ⅴ			-1.75	-1.55
A1E9X5	PsaC	Photosystem Ⅰ subunit Ⅶ			1.56	1.46
C5XNG5	PsaL	Photosystem Ⅰ subunit Ⅺ			-1.62	-1.37
C5XAT4	PsbQ	Photosystem Ⅱ protein			-1.37	-1.04
A1E9Q4	PsbA	Photosystem Ⅱ protein			1.56	1.16
A1E9V0	PsbB	Photosystem Ⅱ protein			1.59	1.18
A1E9Q9	PsbD	Photosystem Ⅱ protein			1.60	1.26
氮代谢 Nitrogen metabolism
C5YWD0	Sb09g016850	AMP deaminase			-1.06	-1.13
C5YCK7	Sb06g023390	Adenylosuccinate lyase			1.32	1.09
C5X4P8	Sb02g029240	Uridine kinase			1.26	1.18
C5YA36	Sb06g032330	Large subunit ribosomal protein LP2			1.49	1.14
C5XIJ4	Sb03g046345	Nonsense-mediated mRNA decay protein 3			1.51	1.58
C5Y6E0	Sb05g024440	Carboxypeptidase			1.09	-1.09
C5Y9S3	Sb06g018740	γ-glutamyl transpeptidase			1.28	1.04
C5XKW6	Sb03g035510	Formamidase			1.09	1.18
C5XPZ6	Sb03g006000	γ-glutamyltranspeptidase			2.23	2.79
登录号 UniProtKB Entry	基因名称 Gene Name		蛋白名称 Protein name	抗病（接种）与抗病（对照） R(JZ) vs. R(CK)		感病（接种）与感病（对照） S(JZ) vs. S(CK)
C5X143	Sb01g008050		β-glucosidase	-1.67		-1.26
C5WUM9	Sb01g031300		5-oxoprolinase (ATP-hydrolysing)	1.12		-1.09
C5WY74	Sb01g005990		Glutathione S-transferase	1.14		2.10
C5WNQ0	Sb01g010540		Glutathione S-transferase	1.68		1.06
C5WUH4	Sb01g030800		Glutathione S-transferase	-1.09		-1.46
C5YIG8	Sb07g005780		Polyamine oxidase	-1.06		-1.69
C5WSJ9	Sb01g029660		Proline dehydrogenase	1.06		-1.16
C5XCA6	Sb02g036990		Amine oxidase	1.20		-1.01
C5Z0N4	SORBI_3009G054600		Dihydrolipoyl dehydrogenase	1.03		-1.07
C5WSJ3	Sb01g029600		3-hydroxyisobutyryl-CoA hydrolase	1.10		-1.03
C5WMY2	Sb01g009880		Glutamte decarboxylase	1.38		1.06
C5YCK7	Sb06g023390		Adenylosuccinate lyase	1.32		1.09
C5YIC3	Sb07g005410		Polyphenol oxidase	1.10		-1.13
C5X371	Sb02g040190		Oxidoreductases	1.33		1.06
C5Z796	SORBI_010G216300		Amine-lyases	2.30		1.23
C5YN64	Sb07g024070		Dihydroxy-acid dehydratase	1.15		1.04
萜类物质代谢Terpenoid backbone biosynthesis
C5XAU4	Sb02g035700		Isopentenyl-diphosphate-δ-isomerase	-1.49		-1.36
C5XHL1	Sb03g032280		Geranyltranstransferase	-1.33		-1.39
C5X0Z0	Sb01g049310		3-hydroxy-3-methylglutaryl coenzyme A synthase	-1.19		-1.54
C5YB59	Sb06g034170		Prenylcysteine oxidase	1.44		1.05
C5X5D5	Sb02g029780		Protein prenyltransferase	1.42		1.13
A0A194YT87	SORBI_004G287300		Geranylgeranyl pyrophosphate synthase	-1.30		-1.12
植物-病原菌互作Plant-pathogen interaction
C5YJ75	SORBI_3007G216300		Probable heat shock protein	-1.37		-1.11
C5X3T9	SORBI_3002G243500		Probable heat shock protein	-1.39		-1.08
C5XEC3	SORBI_3003G226500		SGT1 disease resistance protein homolog1	-1.52		-1.49
C5XBP4	SORBI_3002G343500		Calcium-dependent protein kinase	-1.31		-1.68
C5YMD2	Sb07g001680		Calmodulin	-1.82		-1.77
C5XQS6	SORBI_3003G082600		Calmodulin	1.47		1.39

登录号 UniProtKB Entry	基因名称 Gene Name	蛋白名称 Protein name			抗病（接种）与抗病（对照） R(JZ) vs. R(CK)	感病（接种）与感病（对照） S(JZ) vs. S(CK)
光合作用 Photosynthesis
A1E9R9	AtpH	ATP synthase CF0 C subunit			1.39	-1.13
C5YVW5	Lhcb1	Chlorophyll a-b binding protein			-1.88	-1.65
C5WTC1	Lhcb2	Chlorophyll a-b binding protein			-1.44	-1.38
C5X7M3	Lhcb1	Chlorophyll a-b binding protein			-1.54	-1.26
A1E9U0	PsbF	Cytochrome b6-f complex iron-sulfur subunit			1.54	1.13
C5XL99	PetB	Cytochrome b6-f complex iron-sulfur subunit			1.77	1.28
C5WQF7	PetF	Photosynthetic election transport			-1.38	-1.24
C5YLG2	PetF	Photosynthetic election transport			2.25	1.88
C5X3S4	PsaG	Photosystem Ⅰ subunit Ⅴ			-1.75	-1.55
A1E9X5	PsaC	Photosystem Ⅰ subunit Ⅶ			1.56	1.46
C5XNG5	PsaL	Photosystem Ⅰ subunit Ⅺ			-1.62	-1.37
C5XAT4	PsbQ	Photosystem Ⅱ protein			-1.37	-1.04
A1E9Q4	PsbA	Photosystem Ⅱ protein			1.56	1.16
A1E9V0	PsbB	Photosystem Ⅱ protein			1.59	1.18
A1E9Q9	PsbD	Photosystem Ⅱ protein			1.60	1.26
氮代谢 Nitrogen metabolism
C5YWD0	Sb09g016850	AMP deaminase			-1.06	-1.13
C5YCK7	Sb06g023390	Adenylosuccinate lyase			1.32	1.09
C5X4P8	Sb02g029240	Uridine kinase			1.26	1.18
C5YA36	Sb06g032330	Large subunit ribosomal protein LP2			1.49	1.14
C5XIJ4	Sb03g046345	Nonsense-mediated mRNA decay protein 3			1.51	1.58
C5Y6E0	Sb05g024440	Carboxypeptidase			1.09	-1.09
C5Y9S3	Sb06g018740	γ-glutamyl transpeptidase			1.28	1.04
C5XKW6	Sb03g035510	Formamidase			1.09	1.18
C5XPZ6	Sb03g006000	γ-glutamyltranspeptidase			2.23	2.79
登录号 UniProtKB Entry	基因名称 Gene Name		蛋白名称 Protein name	抗病（接种）与抗病（对照） R(JZ) vs. R(CK)		感病（接种）与感病（对照） S(JZ) vs. S(CK)
C5X143	Sb01g008050		β-glucosidase	-1.67		-1.26
C5WUM9	Sb01g031300		5-oxoprolinase (ATP-hydrolysing)	1.12		-1.09
C5WY74	Sb01g005990		Glutathione S-transferase	1.14		2.10
C5WNQ0	Sb01g010540		Glutathione S-transferase	1.68		1.06
C5WUH4	Sb01g030800		Glutathione S-transferase	-1.09		-1.46
C5YIG8	Sb07g005780		Polyamine oxidase	-1.06		-1.69
C5WSJ9	Sb01g029660		Proline dehydrogenase	1.06		-1.16
C5XCA6	Sb02g036990		Amine oxidase	1.20		-1.01
C5Z0N4	SORBI_3009G054600		Dihydrolipoyl dehydrogenase	1.03		-1.07
C5WSJ3	Sb01g029600		3-hydroxyisobutyryl-CoA hydrolase	1.10		-1.03
C5WMY2	Sb01g009880		Glutamte decarboxylase	1.38		1.06
C5YCK7	Sb06g023390		Adenylosuccinate lyase	1.32		1.09
C5YIC3	Sb07g005410		Polyphenol oxidase	1.10		-1.13
C5X371	Sb02g040190		Oxidoreductases	1.33		1.06
C5Z796	SORBI_010G216300		Amine-lyases	2.30		1.23
C5YN64	Sb07g024070		Dihydroxy-acid dehydratase	1.15		1.04
萜类物质代谢Terpenoid backbone biosynthesis
C5XAU4	Sb02g035700		Isopentenyl-diphosphate-δ-isomerase	-1.49		-1.36
C5XHL1	Sb03g032280		Geranyltranstransferase	-1.33		-1.39
C5X0Z0	Sb01g049310		3-hydroxy-3-methylglutaryl coenzyme A synthase	-1.19		-1.54
C5YB59	Sb06g034170		Prenylcysteine oxidase	1.44		1.05
C5X5D5	Sb02g029780		Protein prenyltransferase	1.42		1.13
A0A194YT87	SORBI_004G287300		Geranylgeranyl pyrophosphate synthase	-1.30		-1.12
植物-病原菌互作Plant-pathogen interaction
C5YJ75	SORBI_3007G216300		Probable heat shock protein	-1.37		-1.11
C5X3T9	SORBI_3002G243500		Probable heat shock protein	-1.39		-1.08
C5XEC3	SORBI_3003G226500		SGT1 disease resistance protein homolog1	-1.52		-1.49
C5XBP4	SORBI_3002G343500		Calcium-dependent protein kinase	-1.31		-1.68
C5YMD2	Sb07g001680		Calmodulin	-1.82		-1.77
C5XQS6	SORBI_3003G082600		Calmodulin	1.47		1.39