Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (5): 898-904.DOI: 10.3969/j.issn.1000-2561.2019.05.010

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Cloning of HbHsfA4a and Its Transcriptional Responses to Cold Stress in Hevea brasiliensis

YU Wencai1,2,3,LI Yan2,WU Shaohua2,CHAO Jinquan2,ZHANG Shixin2,YANG Shuguang2,TIAN Weimin1,2,*()   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Rubber Research Institute, Chinese Academy of Topical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture & Rural Affairs / State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Danzhou, Hainan 571737, China;
    3. Yunnan Institute of Tropical Crops, Jinghong, Yunnan 666100, China
  • Received:2018-08-27 Revised:2019-03-06 Online:2019-05-25 Published:2019-05-31
  • Contact: Weimin TIAN

Abstract:

Heat shock transcription factors (Hsfs) play an important role in the response of plants to abiotic stresses. An Hsf gene, designated as HbHsfA4a, was cloned from a rubber tree (Hevea brasiliensis) clonal line 93-114 by RT-PCR. The ORF of HbHsfA4a was 1215 bp in length which would encode 404 amino acid residues. The putative protein was predicted to possess a molecular mass of 46.40 ku and an isoelectric point of 4.91. Quantitative Real-time PCR (QRT-PCR) analysis showed that the expression of HbHsfA4a was highly induced in the bark tissue of 93-114 by cold stress (4 ℃) and significantly higher than in Reken501 under the same cold treatment. Further analysis showed that the level of the background expression and the expression of HbHsfA4a in response to cold stress within 8 h in the bark tissue of the two cold-tolerant rubber tree clones was significantly higher than that of the two cold-sensitive rubber tree clones. The up-regulation of HbHsfA4a might be crucial to the survival of rubber trees under cold stress.

Key words: Hevea brasiliensis Muell. Arg, heat shock transcription factors, cloning, cold stress, expression analysis

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