Nitrogen Nutrition Characteristics of Rubber Tree Leaves and Its Response to Nitrogen Application Rate

doi:10.3969/j.issn.1000-2561.2019.05.001

Abstract

Abstract:

Rubber tree saplings planted after 2 years were used as the planting material, and a single-factor randomized block design field experiment was conducted to set three treatments with different nitrogen levels (low nitrogen 28 g/plant, medium nitrogen 56 g/plant and high nitrogen 84 g/plant). The parameters of rainfall, daily average temperature, nitrogen content of mature leaves, chlorophyll content and other indicators were dynamically observed for regression analysis and correlation analysis. The nitrogen content of rubber tree leaves showed a decline trend during the annual growth cycle. The difference of nitrogen contents in the leaves between different treatments was not significant. The dynamic change of chlorophyll content in the leaves had obvious seasonal variations, and several peaks were observed throughout the growth cycle. There was no significant chlorophyll content difference between different nitrogen levels. The results of correlation analysis showed that chlorophyll content and total nitrogen content were positively correlated (P<0.05). The nitrogen contents in the leaves was positively correlated with rainfall and temperature, and its correlation with temperature was extremely significant (P<0.01) while its correlation with rainfall was significant (P<0.05). Chlorophyll a, chlorophyll b and chlorophyll content were all extremely correlated with rainfall and temperature (P<0.01). Chlorophyll a/b ratio was extremely negatively correlated with N application rate, and a decrease in chlorophyll a/b ratio was observed with an increase in N rate. Obvious increment of N content in rubber leaves were found after N fertilizer application. Chlorophyll content could be regarded as an indicator revealing N status of rubber leaves to some degree. However, season and climate factors should be considered together.

Key words: nitrogen application rate, rubber tree, nitrogen nutrition, variation

CLC Number:

CHEN Yanbin, ZHANG Yongfa, WANG Wenbin, XUE Xinxin, LUO Xuehua, WU Xiaoping, WANG Dapeng, ZHAO Chunmei. Nitrogen Nutrition Characteristics of Rubber Tree Leaves and Its Response to Nitrogen Application Rate[J]. Chinese Journal of Tropical Crops, 2019, 40(5): 831-838.

Figures/Tables 6

Fig. 1 Daily mean temperature and daily rainfall from May 2017 to Feb. 2018

Fig. 2 Dynamic changes of total N content in leaves of young rubber trees from May 2017 to Feb. 2018 Ⅰ: First fertilization time; Ⅱ: Second fertilization time; Ⅲ: Third fertilization time.

Fig. 3 Dynamic changes of chlorophyll in leaves of young rubber trees from May 2017 to Feb. 2018

Fig. 4 Chlorophyll content rate in different N treatments * indicates significant correlation (P<0.05), ** indicate extremely significant correlation (P<0.01).

Fig. 5 Relationships between chlorophyll content and N content ** indicate extremely significant correlation (P<0.01).

Fig. 6 Relationships between chlorophyll content and, N content and air temperature, daily precipitation * indicates significant correlation (P<0.05), ** indicate extremely significant correlation (P<0.01).

References 29

[1]	何康, 黄宗道 . 热带北缘橡胶树栽培[M]. 广州: 广东科技出版社, 1987: 186-220.
[2]	赵春梅, 蒋菊生, 曹建华 . 橡胶林氮素研究进展[J]. 热带农业科学, 2009,29(3):44-50.
[3]	王文斌, 郭海超, 罗雪华 , 等. 应用 ¹⁵N尿素研究氮素在幼龄橡胶树中吸收和分配特性 [J]. 热带作物学报, 2011,32(1):7-10.
[4]	何向东, 吴小平 . 海南垦区胶园肥力演变探研[J]. 热带农业科学, 2002,22(1):16-22.
[5]	李晶, 姜远茂, 魏绍冲 , 等. 不同施氮水平苹果矮化中间砧幼树光合产物的周年分配利用[J]. 植物营养与肥料学报, 2015,21(3):800-806. DOI
[6]	刘小勇, 王发林, 张坤 , 等. 两个苹果品种不同枝类叶营养含量年周期变化及营养诊断方法研究[J]. 植物营养与肥料学报, 2014,20(2):481-489.
[7]	李德军, 莫江明, 方运霆 , 等. 模拟氮沉降对三种南亚热带树苗生长和光合作用的影响[J]. 生态学报, 2004,24(5):876-882.
[8]	孙金伟, 吴家兵, 任亮 , 等. 氮添加对长白山阔叶红松林2种树木幼苗光合生理生态特征的影响[J]. 生态学报, 2016,36(21):6777-6785.
[9]	Schaberg P G, Perkins T D, Mcnulty S G . Effects of chronic low-level N additions on foliar elemental concentrations, morphology, and gas exchange of mature montane red spruce[J]. Canadian Journal of Forest Research, 1997,27(10):1622-1629. DOI URL
[10]	吴家胜, 应叶青, 曹福亮 , 等. 施氮对银杏叶产量及黄酮含量的影响[J]. 浙江农林大学学报, 2002,19(4):372-375.
[11]	周珺, 林位夫, 王军 , 等. 低N胁迫下巴西橡胶实生苗叶片SPAD值、N素营养及农艺性状的变化[J]. 亚热带植物科学, 2010,39(1):29-33.
[12]	陆行正, 何向东, 吴小平 . 海南省主要胶园土壤配比施肥对胶树生长的影响——橡胶小苗盆栽试验报告[J]. 热带农业科学, 1990,10(4):14-18.
[13]	张宪政 . 植物叶绿素含量测定——丙酮乙醇混合液法[J]. 辽宁农业科学, 1986(3):28-30.
[14]	贝美容, 罗雪华, 杨红竹 . AA3型连续流动分析仪(CFA)同时测定橡胶叶全氮、全磷、全钾的方法研究[J]. 热带作物学报, 2011,32(7):1258-1264.
[15]	Vitousek P M, Howarth R W . Nitrogen limitation on land and in the sea: how can it occur[J]. Biogeochemistry, 1991,13(2):87-115.
[16]	史作民, 唐敬超, 程瑞梅 , 等. 植物叶片氮分配及其影响因子研究进展[J]. 生态学报, 2015,35(18):5909-5919. DOI
[17]	朱海峰, 马建江, 刘艳 , 等. 不同产量库尔勒香梨叶片矿质元素含量年动态变化研究[J]. 新疆农业科学, 2017(3):66-73.
[18]	陈倩, 丁宁, 彭玲 , 等. 供氮水平对矮化苹果 ¹⁵N-尿素吸收、利用、损失及产量和品质的影响 [J]. 应用生态学报, 2017,28(7):2247-2253.
[19]	陆行正, 何向东 . 橡胶树的营养诊断指导施肥[J]. 热带作物学报, 1982,3(1):27-39.
[20]	Kitajima K, Hogan K P . Increases of chlorophyll a/b ratios during acclimation of tropical woody seedlings to nitrogen limitation and high light[J]. Plant, Cell & Environment, 2003,26:857.
[21]	Li J W, Yang J P, Fei P P , et al. Responses of rice leaf thickness, SPAD readings and chlorophyll a/b ratios to different nitrogen supply rates in paddy field[J]. Field Crops Research, 2009,114(3):426-432. DOI URL
[22]	Guo X, Wang R Q, Chang R Y , et al. Effects of nitrogen addition on growth and photosynthetic characteristics of Acer truncatum seedlings[J]. Dendrobiology, 2014,72:151-161. DOI URL
[23]	Lovelock C E, Feller I C, Mckee K L , et al. The effect of nutrient enrichment on growth, photosynjournal and hydraulic conductance of dwarf mangroves in Panamá[J]. Functional Ecology, 2004,18(1):25-33. DOI URL
[24]	Elvir J A, Wiersma G B, Day M E , et al. Effects of enhanced nitrogen deposition on foliar chemistry and physiological processes of forest trees at the Bear Brook Watershed in Maine[J]. Forest Ecology & Management, 2006,221(1-3):207-214.
[25]	Ji D H, Mao Q Z, Watanabe Y , et al. Effect of nitrogen loading on the growth and photosynthetic responses of Japanese larch seedlings grown under different light regimes[J]. Journal of Agricultural Meteorology, 2015,71(3):232-238. DOI URL
[26]	Raese J T, Drake S R, Curry E A . Nitrogen fertilizer influences fruit quality, soil nutrients and cover crops, leaf color and nitrogen content, biennial bearing and cold hardiness of ‘Golden Delicious’[J]. Journal of Plant Nutrition, 2007,30(10):1585-1604. DOI URL
[27]	钟小莉, 马晓东, 吕豪豪 , 等. 干旱胁迫下氮素对胡杨幼苗生长及光合的影响[J]. 生态学杂志, 2017,36(10):2777-2786.
[28]	彭礼琼, 金则新, 王强 . 模拟氮沉降对夏蜡梅幼苗生理生态特性的影响[J]. 生态学杂志, 2014,33(4):989-995.
[29]	李娟, 安锋, 林位夫 , 等. 不同氮磷钾肥用量对五指毛桃叶绿素含量的影响[J]. 核农学报, 2016,30(1):193-200.