Effects of Paclobutrazol on Soil Bacterial Diversity in Mango Orchard and PICRUSt-based Predicted Metagenomic Analysis
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 807-814.DOI: 10.3969/j.issn.1000-2561.2019.04.027

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Effects of Paclobutrazol on Soil Bacterial Diversity in Mango Orchard and PICRUSt-based Predicted Metagenomic Analysis

JIN Xiaotuo1,ZHOU Yanyu1,XIA Yangrongchang1,CHEN Lijun2,LI Tao2,ZHAO Hongwei1,*()   

  1. 1. College of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China
    2. Hainan Plant Protection Station, Haikou, Hainan 570100, China
  • Received:2018-11-16 Revised:2019-01-15 Online:2019-04-15 Published:2019-05-21
  • Contact: Hongwei ZHAO


The Illumina Miseq 16S rRNA high-throughput sequencing technology was used to study the bacterial community structure and diversity of two group soil samples with and without paclobutrazol treatment, respectively, from a mango orchard in Ledong, Hainan. A total of 3586 operational taxonomic units (OTUs) in two groups were obtained, which were classified as 38 phylums, 89 classes, 195 orders, 378 families, 673 genera and 1353 species. The results of the analysis of Alpha diversity showed that soil bacterial richness index was significantly higher in the paclobutrazo-treated soils, however compared with the soils without paclobutrazol treatment, the bacterial diversity was significantly decreased. The principal component analysis indicated that the community structure of soil bacteria was affected by paclobutrazol. The quantities of Proteobacteria and Planctomycetes were significantly increased in the paclobutrazol-treated soils, however, those of Actinobacteria and Firmicutes were significantly decreased. The PICRUSt analysis showed that the functional gene families were mainly related to 43 sub-functions including the cell growth and death, carbohydrate metabolism, biosynthesis of secondary metabolites, amino acid metabolism, and so on. The total metabolic capacity of soil bacteria could be reduced after treated with paclobutrazol. Therefore, the application of paclobutrazol can reduce the diversity of soil bacteria, and it also has some impacts on the relative abundance and functions of soil bacteria.

Key words: paclobutrazol, mango orchard, soil, bacterial diversity, PICRUSt analysis