Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 753-757.DOI: 10.3969/j.issn.1000-2561.2019.04.020

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Determination of Imidacloprid and β-cypermethrin in Banana by UPLC-MS/MS

WANG Mimi1,2,XIE Defang1,2,*()   

  1. 1. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
  • Received:2018-07-25 Revised:2019-01-25 Online:2019-04-15 Published:2019-05-21
  • Contact: Defang XIE

Abstract:

A method was established for the simultaneous determination of imidacloprid and β-cypermethrin residues in bananas. The samples were extracted with 1% of acetic acid-acetonitrile, cleaned by PSA and GCB. Gradient elution separation was carried out with methanol and 2 mmol/L ammonium acetate solution as the mobile phase. The samples were determined using electrospray positive ionization (ESI +) with multiple reaction monitoring (MRM) and then quantified by the matrix-matched external standard method. The results showed that the correlation coefficient r was > 0.9991, when the concentration ranged from 0.001 to 0.1 mg/L. The limit of detection (LOD) was 1 pg and the limit of quantification (LOQ) was 0.01 mg/kg. The recovery of imidacloprid and β-cypermethrin in banana was from 84.91% to 104.78% and the relative standard deviation (RSD) was from 1.23% to 9.69%. The method is simple, sensitive and reproducible for the determination of imidacloprid and β-cypermethrin residues in bananas.

Key words: banana, imidacloprid, β-cypermethrin;, UPLC-MS/MS