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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 734-740.DOI: 10.3969/j.issn.1000-2561.2019.04.017
• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles Next Articles
WANG Xiaoyu,LI Zengping(
),ZHENG Zhilin,ZHANG Yu,WU Ruhui
Received:2018-11-20
Revised:2019-01-19
Online:2019-04-15
Published:2019-05-21
Contact:
Zengping LI
WANG Xiaoyu,LI Zengping,ZHENG Zhilin,ZHANG Yu,WU Ruhui. Identification and Biological Characteristics of Corynespora cassiicola Causing Corynespora Leaf Spot on Nelumbo nucifera[J]. Chinese Journal of Tropical Crops, 2019, 40(4): 734-740.
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Fig. 1 The symptoms of Corynespora leaf spot on N. nucifera A: Natural symptoms; B: Premonitory symptoms; C: Medium symptoms; D: Final symptoms.
Fig. 2 Pathogenicity testing of Corynespora leaf spot on N. nucifera
Fig. 3 Infection of other plants by the pathogen of Corynespora leaf spot on N. nucifera A: Leaf symptoms of Hevea brasiliensis; B: Leaf symptoms of Manihot esculenta; C: Leaf symptoms of Solanum melongena; D: Leaf symptoms of Carica papaya.
Fig. 4 Morphological of pathogen conidium A: Pathogen’s colony morphology on PDA medium in 30 ℃ for 10 d; B: Conidiophore; C: Conidium; D: Germinal conidium.
Fig. 5 Molecular identification of the pathogen of Corynespora leaf spot on N. nucifera
Fig. 6 Colony diameter of pathogen cultured at different temperature Different capital letters mean extremely significant difference at 0.01 level, and different lowercase letters mean significant difference at 0.05 level.
Fig. 7 Colony diameter of pathogen cultured at different pH value Different capital letters mean extremely significant difference at 0.01 level, and different lowercase letters mean significant difference at 0.05 level.
| 碳源 Carbon source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
|---|---|---|
| 葡萄糖Glucose | 7.72±0.19aA | 菌丝稀薄,产生色素少 |
| D-木糖D-Xylose | 7.67±0.08aA | 菌丝稀薄,产生色素少 |
| D-半乳糖 D-Galactose | 7.10±0.22bB | 菌丝厚度中等,产生色素中等 |
| α-乳糖α-Lactose | 6.58±0.15cC | 菌丝稀薄,产生色素少 |
| 麦芽糖Maltose | 6.30±0.22cC | 菌丝稀薄,产生色素少 |
| 甘露醇Mannitol | 5.42±0.45dD | 菌丝稀薄,产生色素少 |
| 碳源对照CK | 5.20±0.28deDE | 菌丝最薄,产生色素少 |
| D-果糖 D-Fructose | 5.20±0.13edDE | 菌丝厚,产生色素多 |
| D-山梨醇 D-Sorbitol | 5.08±0.10defDE | 菌丝稀薄,产生色素少 |
| 可溶性淀粉 Soluble starch | 4.92±0.13efDE | 菌丝厚度中等,产生色素少 |
| 蔗糖Sucrose | 4.77±0.13fEF | 菌丝厚,产生色素多 |
| 肌醇Inositol | 4.32±0.13gF | 菌丝稀薄,产生色素中等 |
| 甘油Glycerinum | 4.27±0.33gF | 菌丝稀薄,产生色素少 |
Tab. 1 Colony diameter and colony characteristics of pathogen under different carbon sources conditions
| 碳源 Carbon source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
|---|---|---|
| 葡萄糖Glucose | 7.72±0.19aA | 菌丝稀薄,产生色素少 |
| D-木糖D-Xylose | 7.67±0.08aA | 菌丝稀薄,产生色素少 |
| D-半乳糖 D-Galactose | 7.10±0.22bB | 菌丝厚度中等,产生色素中等 |
| α-乳糖α-Lactose | 6.58±0.15cC | 菌丝稀薄,产生色素少 |
| 麦芽糖Maltose | 6.30±0.22cC | 菌丝稀薄,产生色素少 |
| 甘露醇Mannitol | 5.42±0.45dD | 菌丝稀薄,产生色素少 |
| 碳源对照CK | 5.20±0.28deDE | 菌丝最薄,产生色素少 |
| D-果糖 D-Fructose | 5.20±0.13edDE | 菌丝厚,产生色素多 |
| D-山梨醇 D-Sorbitol | 5.08±0.10defDE | 菌丝稀薄,产生色素少 |
| 可溶性淀粉 Soluble starch | 4.92±0.13efDE | 菌丝厚度中等,产生色素少 |
| 蔗糖Sucrose | 4.77±0.13fEF | 菌丝厚,产生色素多 |
| 肌醇Inositol | 4.32±0.13gF | 菌丝稀薄,产生色素中等 |
| 甘油Glycerinum | 4.27±0.33gF | 菌丝稀薄,产生色素少 |
| 氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
|---|---|---|
| 牛肉膏 Beef extract | 7.50±0.10aA | 菌丝厚,产生色素多 |
| 大豆蛋白胨 Soy peptone | 7.30±0.13aA | 菌丝厚,产生色素多 |
| 氮源对照CK | 7.23±0.06aA | 菌丝最薄,产生色素少 |
| 酵母浸膏 Yeast extract | 6.80±0.09bB | 菌丝厚,产生色素中等 |
| 硝酸钙 Ca(NO3)2 | 6.78±0.23bB | 菌丝稀薄,产生色素少 |
| 硝酸钠 NaNO3 | 5.77±0.21cC | 菌丝厚,产生色素中等 |
| 草酸铵 (NH4)2C2O4 | 5.40±0.05cCD | 菌丝厚,产生色素多 |
| 硝酸钾 KNO3 | 5.27±0.21dD | 菌丝厚度中等,产生色素中等 |
| L-天冬酰胺 L-Asparagine | 5.23±0.08dD | 菌丝厚度中等,产生色素中等 |
| 氯化铵NH4Cl | 4.52±0.18eE | 菌丝厚,产生色素多 |
| 硝酸铵NH4NO3 | 4.28±0.35eE | 菌丝厚,产生色素多 |
| 硫酸铵 (NH4)2SO4 | 3.62±0.08fF | 菌丝厚,产生色素多 |
Tab. 2 Colony diameter and colony characteristics of pathogen under different nitrogen sources conditions
| 氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
|---|---|---|
| 牛肉膏 Beef extract | 7.50±0.10aA | 菌丝厚,产生色素多 |
| 大豆蛋白胨 Soy peptone | 7.30±0.13aA | 菌丝厚,产生色素多 |
| 氮源对照CK | 7.23±0.06aA | 菌丝最薄,产生色素少 |
| 酵母浸膏 Yeast extract | 6.80±0.09bB | 菌丝厚,产生色素中等 |
| 硝酸钙 Ca(NO3)2 | 6.78±0.23bB | 菌丝稀薄,产生色素少 |
| 硝酸钠 NaNO3 | 5.77±0.21cC | 菌丝厚,产生色素中等 |
| 草酸铵 (NH4)2C2O4 | 5.40±0.05cCD | 菌丝厚,产生色素多 |
| 硝酸钾 KNO3 | 5.27±0.21dD | 菌丝厚度中等,产生色素中等 |
| L-天冬酰胺 L-Asparagine | 5.23±0.08dD | 菌丝厚度中等,产生色素中等 |
| 氯化铵NH4Cl | 4.52±0.18eE | 菌丝厚,产生色素多 |
| 硝酸铵NH4NO3 | 4.28±0.35eE | 菌丝厚,产生色素多 |
| 硫酸铵 (NH4)2SO4 | 3.62±0.08fF | 菌丝厚,产生色素多 |
Fig. 8 Colony diameter of pathogen culture under different light conditions The same letter means no significant difference.
| [1] |
盛尊科 . 鱼台县莲藕常见病害综合防治技术[J]. 吉林蔬菜, 2017(6):24-25.
DOI URL |
| [2] | 杨文成, 吕环照, 曾幼民 , 等. 莲藕褐斑病的初步研究[J]. 江西农业科技, 1992(6):28-29. |
| [3] | 余松华 . 莲藕褐斑病的发生与防治技术[J]. 中国果菜, 2016,36(8):60-61. |
| [4] |
马小三 . 莲藕褐斑病的发生与防治[J]. 甘肃农业, 2010(5):94.
DOI URL |
| [5] | 王红霞, 阎河 . 莲藕褐斑病的发生与防治[J]. 农村百事通, 2003(24):33. |
| [6] |
丁自立, 吴金平, 刁英 , 等. 莲藕主要病害及其综合防治[J]. 湖北农业科学, 2011,50(15):3078-3079, 3082.
DOI URL |
| [7] |
陈雯, 陈夕军, 陈春风 , 等. 我国水生蔬菜主要病害研究进展[J]. 长江蔬菜, 2017,18(21):72-76.
DOI URL |
| [8] | 方中达 . 植病研究方法[M]. 北京: 中国农业出版社, 1998: 1-427. |
| [9] | 孙广宇, 宗兆锋 . 植物病理学实验技术[M]. 北京: 中国农业出版社, 2002. |
| [10] | 陆家云 . 植物病原真菌学[M]. 北京: 中国农业出版社, 2001: 407-408. |
| [11] |
王惠哲, 邓强, 曹明明 , 等. 黄瓜棒孢叶斑病菌的分子鉴定及rDNA-ITS序列分析[J]. 华北农学报, 2015,30(3):175-179.
DOI |
| [12] | 吴如慧, 李增平, 孙先伊晴 , 等. 橡胶树臭根病菌的鉴定及其生物学特性研究[J]. 热带作物学报, 2018,39(5):940-947. |
| [13] | Hieu N D, Nghia N A, Chi V T Q , et al. Genetic diversity and pathogenicity of Corynespora cassiicola isolates from rubber trees and other hosts in vietnam[J]. Journal of Rubber Research, 2014,17(3):187-203. |
| [14] | 李宝聚, 高苇, 石延霞 , 等. 多主棒孢和棒孢叶斑病的研究进展[J]. 植物保护学报, 2012,39(2):171-176. |
| [15] | 肖敏, 曾向萍, 严婉荣 , 等. 节瓜褐斑病病原鉴定及生物学特性[J]. 分子植物育种, 2016,14(11):3183-3189. |
| [16] |
秦人全, 林江, 王洪星 , 等. 木棉褐斑病病原菌鉴定及生物学特性[J]. 江苏农业科学, 2017,45(9):101-104.
DOI URL |
| [17] |
张贺, 蒲金基, 张欣 , 等. 巴西橡胶树棒孢霉落叶病病原菌的生物学特性[J]. 热带作物学报, 2007,28(3):83-87.
DOI URL |
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