Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 734-740.DOI: 10.3969/j.issn.1000-2561.2019.04.017
• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles Next Articles
WANG Xiaoyu,LI Zengping(),ZHENG Zhilin,ZHANG Yu,WU Ruhui
Received:
2018-11-20
Revised:
2019-01-19
Online:
2019-04-15
Published:
2019-05-21
Contact:
Zengping LI
WANG Xiaoyu,LI Zengping,ZHENG Zhilin,ZHANG Yu,WU Ruhui. Identification and Biological Characteristics of Corynespora cassiicola Causing Corynespora Leaf Spot on Nelumbo nucifera[J]. Chinese Journal of Tropical Crops, 2019, 40(4): 734-740.
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URL: http://www.rdzwxb.com/EN/10.3969/j.issn.1000-2561.2019.04.017
Fig. 3 Infection of other plants by the pathogen of Corynespora leaf spot on N. nucifera A: Leaf symptoms of Hevea brasiliensis; B: Leaf symptoms of Manihot esculenta; C: Leaf symptoms of Solanum melongena; D: Leaf symptoms of Carica papaya.
Fig. 4 Morphological of pathogen conidium A: Pathogen’s colony morphology on PDA medium in 30 ℃ for 10 d; B: Conidiophore; C: Conidium; D: Germinal conidium.
Fig. 6 Colony diameter of pathogen cultured at different temperature Different capital letters mean extremely significant difference at 0.01 level, and different lowercase letters mean significant difference at 0.05 level.
Fig. 7 Colony diameter of pathogen cultured at different pH value Different capital letters mean extremely significant difference at 0.01 level, and different lowercase letters mean significant difference at 0.05 level.
碳源 Carbon source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
---|---|---|
葡萄糖Glucose | 7.72±0.19aA | 菌丝稀薄,产生色素少 |
D-木糖D-Xylose | 7.67±0.08aA | 菌丝稀薄,产生色素少 |
D-半乳糖 D-Galactose | 7.10±0.22bB | 菌丝厚度中等,产生色素中等 |
α-乳糖α-Lactose | 6.58±0.15cC | 菌丝稀薄,产生色素少 |
麦芽糖Maltose | 6.30±0.22cC | 菌丝稀薄,产生色素少 |
甘露醇Mannitol | 5.42±0.45dD | 菌丝稀薄,产生色素少 |
碳源对照CK | 5.20±0.28deDE | 菌丝最薄,产生色素少 |
D-果糖 D-Fructose | 5.20±0.13edDE | 菌丝厚,产生色素多 |
D-山梨醇 D-Sorbitol | 5.08±0.10defDE | 菌丝稀薄,产生色素少 |
可溶性淀粉 Soluble starch | 4.92±0.13efDE | 菌丝厚度中等,产生色素少 |
蔗糖Sucrose | 4.77±0.13fEF | 菌丝厚,产生色素多 |
肌醇Inositol | 4.32±0.13gF | 菌丝稀薄,产生色素中等 |
甘油Glycerinum | 4.27±0.33gF | 菌丝稀薄,产生色素少 |
Tab. 1 Colony diameter and colony characteristics of pathogen under different carbon sources conditions
碳源 Carbon source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
---|---|---|
葡萄糖Glucose | 7.72±0.19aA | 菌丝稀薄,产生色素少 |
D-木糖D-Xylose | 7.67±0.08aA | 菌丝稀薄,产生色素少 |
D-半乳糖 D-Galactose | 7.10±0.22bB | 菌丝厚度中等,产生色素中等 |
α-乳糖α-Lactose | 6.58±0.15cC | 菌丝稀薄,产生色素少 |
麦芽糖Maltose | 6.30±0.22cC | 菌丝稀薄,产生色素少 |
甘露醇Mannitol | 5.42±0.45dD | 菌丝稀薄,产生色素少 |
碳源对照CK | 5.20±0.28deDE | 菌丝最薄,产生色素少 |
D-果糖 D-Fructose | 5.20±0.13edDE | 菌丝厚,产生色素多 |
D-山梨醇 D-Sorbitol | 5.08±0.10defDE | 菌丝稀薄,产生色素少 |
可溶性淀粉 Soluble starch | 4.92±0.13efDE | 菌丝厚度中等,产生色素少 |
蔗糖Sucrose | 4.77±0.13fEF | 菌丝厚,产生色素多 |
肌醇Inositol | 4.32±0.13gF | 菌丝稀薄,产生色素中等 |
甘油Glycerinum | 4.27±0.33gF | 菌丝稀薄,产生色素少 |
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
---|---|---|
牛肉膏 Beef extract | 7.50±0.10aA | 菌丝厚,产生色素多 |
大豆蛋白胨 Soy peptone | 7.30±0.13aA | 菌丝厚,产生色素多 |
氮源对照CK | 7.23±0.06aA | 菌丝最薄,产生色素少 |
酵母浸膏 Yeast extract | 6.80±0.09bB | 菌丝厚,产生色素中等 |
硝酸钙 Ca(NO3)2 | 6.78±0.23bB | 菌丝稀薄,产生色素少 |
硝酸钠 NaNO3 | 5.77±0.21cC | 菌丝厚,产生色素中等 |
草酸铵 (NH4)2C2O4 | 5.40±0.05cCD | 菌丝厚,产生色素多 |
硝酸钾 KNO3 | 5.27±0.21dD | 菌丝厚度中等,产生色素中等 |
L-天冬酰胺 L-Asparagine | 5.23±0.08dD | 菌丝厚度中等,产生色素中等 |
氯化铵NH4Cl | 4.52±0.18eE | 菌丝厚,产生色素多 |
硝酸铵NH4NO3 | 4.28±0.35eE | 菌丝厚,产生色素多 |
硫酸铵 (NH4)2SO4 | 3.62±0.08fF | 菌丝厚,产生色素多 |
Tab. 2 Colony diameter and colony characteristics of pathogen under different nitrogen sources conditions
氮源 Nitrogen source | 菌落直径 Colony diameter/cm | 菌落形态 Colony characteristics |
---|---|---|
牛肉膏 Beef extract | 7.50±0.10aA | 菌丝厚,产生色素多 |
大豆蛋白胨 Soy peptone | 7.30±0.13aA | 菌丝厚,产生色素多 |
氮源对照CK | 7.23±0.06aA | 菌丝最薄,产生色素少 |
酵母浸膏 Yeast extract | 6.80±0.09bB | 菌丝厚,产生色素中等 |
硝酸钙 Ca(NO3)2 | 6.78±0.23bB | 菌丝稀薄,产生色素少 |
硝酸钠 NaNO3 | 5.77±0.21cC | 菌丝厚,产生色素中等 |
草酸铵 (NH4)2C2O4 | 5.40±0.05cCD | 菌丝厚,产生色素多 |
硝酸钾 KNO3 | 5.27±0.21dD | 菌丝厚度中等,产生色素中等 |
L-天冬酰胺 L-Asparagine | 5.23±0.08dD | 菌丝厚度中等,产生色素中等 |
氯化铵NH4Cl | 4.52±0.18eE | 菌丝厚,产生色素多 |
硝酸铵NH4NO3 | 4.28±0.35eE | 菌丝厚,产生色素多 |
硫酸铵 (NH4)2SO4 | 3.62±0.08fF | 菌丝厚,产生色素多 |
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