Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 715-723.DOI: 10.3969/j.issn.1000-2561.2019.04.014

• Biotechnology and Tissue Cultures • Previous Articles     Next Articles

Rapid Propagation of Standard Cut Chrysanthemum ‘Baishan’

ZHOU Ting1,2,YANG Huiting1,HU Jihong1,ZHU Mengzhu1,HONG Rongqin3,PAN Dongming1,SHE Wenqin1,**(),CHEN Guixin1,**()   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Forest Seedling General Station of Fujian Province, Fuzhou, Fujian 350002, China
    3. Youxi Sanye Flower Limited Company, Youxi, Fujian 365100, China
  • Received:2018-06-12 Revised:2018-11-22 Online:2019-04-15 Published:2019-05-21
  • Contact: Wenqin SHE,Guixin CHEN


Using terminal buds and stems with axillary buds as the explants, the technology of tissue culture and rapid propagation of standard cut chrysanthemum ‘Baishan’ was studied. The best sterilization conditions of apical bud explants and axillary buds was 75% alcohol 30 s+0.1% HgCl2 for 3 min and 4 min. The best primary culture medium of ‘Baishan’ was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L with the best inducing effect rate 100% and average single bud induction 1.69. The optimal proliferation medium was MS+6-BA 1.5 mg/L+NAA 0.15 mg/L with multiplication coefficient 3.05, effective rate of bud 65.26% and average height 3.22 cm cultivated 30 d after. The 1/2MS medium supplemented with NAA and IBA could induce rooting in bottles, and the rooting rate was 100%. It could root outside bottles. The buds was inserted into the substrate after 5 minutes of immersion in the NAA solution, and the rooting condition was good. The results could be applied directly for production popularization of ‘Banshan’. The results could provide theoretical and technical guidance for the large-scale promotion and industrialization of ‘Baishan’ and standardized production of seedlings.

Key words: standard cut chrysanthemum, Chrysanthemum ‘Baishan’, tissue culture and rapid propagation