Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 701-707.DOI: 10.3969/j.issn.1000-2561.2019.04.012

• Biotechnology and Tissue Cultures • Previous Articles     Next Articles

Cloning and Expression Analysis of Cassava MeNAC29 and MeNAC30 Genes

LIN Zhaowei1,LI Chaoping2,SHI Tao2,WANG Guofen2,LI Boxun2,CAI Jimiao2,HUANG Guixiu2,*()   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2018-10-22 Revised:2019-02-22 Online:2019-04-15 Published:2019-05-21
  • Contact: Guixiu HUANG


The NAC (NAM/ATAF/CUC) transcription factor gene family was widely distributed in terrestrial plants and plays an important role in the regulation of growth and stress responses. The preliminary research about the role of this gene family on the cassava against bacterial blight was carried out in our lab. Two genes, MeNAC29 and MeNAC30, were cloned from cassava cDNA by RT-PCR, the different expression changes between resistant and susceptible cassava germplasms infected by Xanthomonas axonopodis pv. manihotis (Xam) were also quantified by qRT-PCR. The results showed both MeNAC29 and MeNAC30 contain three exons, two introns, and conserved NAM domain. The length of MeNAC29 gene’s ORF was 888 nt, which encode 295 amino acids, while the MeNAC30 gene’s ORF was 870 nt and encode 289 amino acids. Compared with susceptible cassava germplasms, both two genes’ expression was significantly increased in the resistant cassava germplasms - Xanthomonas axonopodis pv. manihotis interaction, which showed they are involved in resistance response of cassava to bacterial blight.

Key words: cassava, MeNAC29 gene, MeNAC30 gene, expression analysis