Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 681-687.DOI: 10.3969/j.issn.1000-2561.2019.04.009

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Relationship Between Sucrose Content and Sucrose Metabolism Enzymes in the High and Low Sugar Sugarcane at Seedling Stage

NIU Junqi1,2,3,MIAO Xiaorong1,2,3,HUANG Wei1,*(),YANG Litao3,4,*(),LI Yangrui3,4   

  1. 1. College of Biology and Pharmacy, Yulin Normal University, Yulin, Guangxi 537000, China
    2. Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin, Guangxi 537000, China
    3. College of Agriculture, Guangxi University, Nanning, Guangxi 530005, China
    4. Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
  • Received:2018-07-30 Revised:2019-02-03 Online:2019-04-15 Published:2019-05-21
  • Contact: Wei HUANG,Litao YANG

Abstract:

The stems and leaves of different maturity at the seedling stage of high sugar (GT35) and low sugar (B8) sugarcane were used as the materials, and the contents of sucrose, glucose and fructose were measured using high performance liquid chromatography (HPLC), to analyze the correlation and difference between sucrose metabolic enzymes and sugar accumulation in high and low sugar sugarcane. The sucrose content in the leaves was positively correlated with the activity of sucrose phosphate synthase (SPS) and sucrose synthase in the synthesis direction (SS-s). The hexose content in the stem was positively correlated with the activity of neutral/alkaline invertase (NI), the sucrose content in the stem was positively correlated with the activity of SPS, and the activity of sucrose synthetase in the cleavage direction (SS-c), soluble acid invertase (SAI) and cell wall-bound invertase (CIN) was negatively correlated. Sucrose content in the stem of GT35 was significantly higher than that of B8, and sucrose content in the leaf was significantly lower than that of B8. The SPS activity in stems and SS-s activity leaves of GT35 were significantly higher than that of B8, while the SS-s and SS-c activity in GT35 stems was lower than that of B8, but only the SS-c activity in young stems of GT35 was not significantly lower than of B8. It indicates that high SS-s activity in leaves, high SPS activity in stems, low SS-s and SS-c activity in stems may be important factors for regulating sucrose accumulation in high-sugar varieties of sugarcane at seedling stage.

Key words: sugarcane cultivars, sucrose metabolism enzymes, sucrose accumulation, correlation analysis