Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 675-680.DOI: 10.3969/j.issn.1000-2561.2019.04.008

• Phytophysiology and Biochemistry • Previous Articles     Next Articles

Changes of Sugar Contents and Related Enzyme Activities During Somatic Embryogenesis of Two Lychee Varieties

JI Xunzhi1,2,LI Huanling1,WANG Guo1,GAO Zhaoyin1,SUN Jinhua1,WANG Jiabao1,*()   

  1. 1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan 571101, China
    2. Beverage and Spice Research Institute, Chinese Academy of Tropical Agriculture Sciences, Wanning, Hainan 571533, China
  • Received:2018-07-30 Revised:2019-02-03 Online:2019-04-15 Published:2019-05-21
  • Contact: Jiabao WANG

Abstract:

In order to investigate the effect of sugar metabolism on the somatic embryogenesis of litchi, embryonic callus of ‘Dadingxiang’ and ‘Xinqiumili’ were inoculated on TD medium, respectively, and the embryonic callus of ‘Xinqiumili’ was inoculated on TX medium. Contents of starch, sucrose and other sugar components and activities of the metabolism-related enzymes were compared among the three embryonic cultures. The results showed that starch content on TD and TX medium in ‘Xinqiumili’ were lower than that in ‘Dadingxiang’ during somatic embryogenesis. All the glucose and fructose content were similar, but glucose and fructose contents in ‘Dadingxiang’ was higher than that in ‘Xinqiumili’, while sucrose content in ‘Xinqiumili’ was higher than that in ‘Dadingxiang’. The activity of amylase and β-amylase in ‘Xinqiumili’ were higher than that in ‘Dadingxiang’, but the α-amylase activity in ‘Xinqiumili’ and ‘Dadingxiang’ were similar. The activity of sucrose synthase and acid invertase on TD medium in ‘Xinqiumili’ were higher than that in ‘Dadingxiang’, but neutral invertase activity in ‘Dadingxiang’ was higher than that in ‘Xinqiumili’, and also the dominant by neutral invertase on TX medium in ‘Xinqiumili’. Through converted the starch into the sucrose by amylase, sucrose synthase and invertase in ‘Xinqiumili’ was provided material energy for somatic embryogenesis, while high neutral invertase activity and low amylase activity in ‘Dadingxiang’ can not accumulation of sucrose, maked the efficiency of somatic embryogenesis in ‘Dadingxiang’ was lower than that in ‘Xinqiumili’.

Key words: litchi (Litchi chinensis Sonn.), somatic embryogenesis, carbohydrate metabolism, enzyme activity