Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (4): 638-648.DOI: 10.3969/j.issn.1000-2561.2019.04.003

• Crop Culture and Nutrition,Genetic Breeding • Previous Articles     Next Articles

Evaluation of Cold Tolerance of Saccharum spontaneum L. Clones Collected from Different Habitats

YANG Yang,WANG Xianhong(),YANG Qinghui()   

  1. Sugarcane Research Institution, Yunnan Agricultural University, Kunming, Yunnan 650201, China
  • Received:2018-06-06 Revised:2019-01-16 Online:2019-04-15 Published:2019-05-21
  • Contact: Xianhong WANG,Qinghui YANG

Abstract:

To screen cold tolerance of wild sugarcane germplasms, and to explore the relationship between cold tolerance with original altitude and latitude, the comprehensive evaluation of cold tolerance of clones of Saccharum spontaneum L. from different habitats were performed. After low temperature stress at seedling stage, seven physiological indexes of 40 S. spontaneum clones samples were evaluated using cluster analysis, fuzzy membership function analysis and correlation analysis. Compared with the control group, the contents of Pro, MDA, PMP and SP in the stressed clones increased, in which the content of Pro was the highest by increasing 43.81%. The contents of Chl, SOD and POD decreased, moreover the content of Chl decreased the greatest by 35.01%. The coefficients of variation of Chl, Pro and SOD increased in different degrees, and the biggest increase was found in Chl, which was 1.76 times of the control. The result of correlation analysis showed that the cold tolerance of the 40 S. spontaneum clones was significantly positively correlated with the altitude and latitude of the habitats by correlation coefficients, which was 0.867 and 0.686, respectively. All the 40 S. spontaneum clones were divided into three categories according to the cold tolerance using fuzzy membership function and cluster analysis, in which 10 clones showed high cold tolerance, 19 clones showed medium cold tolerance and 11 clones were cold sensitive. The results showed that there were significant differences in cold tolerance among S. spontaneum clones collected from different habitats, and the membership function of cold tolerance was positively correlated with altitude and latitude, namely the clones collected from high altitude and high latitude had stronger cold tolerance than that collected from low altitude and low latitude habitats. It indicated that latitude is the main factor for the cold tolerance of S. spontaneum clones collected from the same altitude, and altitude is the main factor for the cold tolerance of S. spontaneum clones collected from the same latitude. This work would provide the scientific basis for the further utilization of S. spontaneum clones in sugarcane cold tolerance breeding.

Key words: Saccharum spontaneum L., cold tolerance, fuzz membership function, altitude, latitude