Pathogen Identification and Fungicide-screening of the Peanut Black Rot

doi:10.3969/j.issn.1000-2561.2019.03.020

Abstract

Abstract:

Peanut black rot is an severe disease on peanuts, which has a serious impact on production. It is also a quarantine plant disease in China. To identify the pathogen of peanut rot and get the effective fungicide which can inhibit the mycelium growth of the pathogen, the pathogen of peanut black rot was identified by colony characteristics, morphological characteristics and DNA sequence analysis. The inhibitory effect of 8 fungicides on C. iliciola was determined by the mycelium growth rate method. The results showed that the pathogen of peanut black rot was Calonectria iliciola. 10% Tebuconazole was the best fungicide to inhibit the mycelium growth of C. iliciola, and the EC₅₀ value was 1.12 μg/mL. 300 g/L Difenoconazole·propiconazol EC, 29% isopyrazam·azoxystrobin SC, 25% Fludioxonil SC, 15% pyraclostrobin SC and 22.5% picoxystrobin could inhibit SC the mycelium growth of C. iliciola, and the EC₅₀value was under 10 μg/mL. 30% metalaxy-hymexazol was the worst fungicide to the mycelium growth of C. iliciola and the EC₅₀ value was 76.71 μg/mL. The results above could provide a theoretical basis for the prevention and control of black rot of peanut.

Key words: peanut, black rot, fungicide, toxicity test

LI Na,WANG Zetian,HE Guibi,XIANG Meimei,ZHANG Yunxia. Pathogen Identification and Fungicide-screening of the Peanut Black Rot[J]. Chinese Journal of Tropical Crops, 2019, 40(3): 552-557.

Figures/Tables 4

References 18

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供试药剂 Fungicide	毒力回归方程 Toxicity regression equation	抑制中浓度EC₅₀/(μg?mL^-1)	95%抑制浓度 EC₉₅/(μg?mL^-1)	相关系数R Correlation coefficient
精甲噁霉灵	Y=3.2367+0.9355X	76.71	4396.92	0.9887
戊唑醇	Y=4.9546+0.9422X	1.12	62.23	0.9927
啶氧菌酯	Y=4.4997+0.6673X	5.62	1638.58	0.9893
噻夫吡唑醚	Y=4.1808+0.8135X	10.16	1069.10	0.9913
吡唑嘧菌酯	Y=4.5030+0.6781X	5.41	1441.65	0.9944
吡萘嘧菌酯	Y=4.4936+1.2096X	2.62	60.25	0.9720
苯甲·丙环唑	Y=4.8089+0.8396X	1.69	153.75	0.9991
咯菌腈	Y=4.5718+0.7001X	4.09	914.03	0.9892

供试药剂 Fungicide	毒力回归方程 Toxicity regression equation	抑制中浓度EC₅₀/(μg?mL^-1)	95%抑制浓度 EC₉₅/(μg?mL^-1)	相关系数R Correlation coefficient
精甲噁霉灵	Y=3.2367+0.9355X	76.71	4396.92	0.9887
戊唑醇	Y=4.9546+0.9422X	1.12	62.23	0.9927
啶氧菌酯	Y=4.4997+0.6673X	5.62	1638.58	0.9893
噻夫吡唑醚	Y=4.1808+0.8135X	10.16	1069.10	0.9913
吡唑嘧菌酯	Y=4.5030+0.6781X	5.41	1441.65	0.9944
吡萘嘧菌酯	Y=4.4936+1.2096X	2.62	60.25	0.9720
苯甲·丙环唑	Y=4.8089+0.8396X	1.69	153.75	0.9991
咯菌腈	Y=4.5718+0.7001X	4.09	914.03	0.9892