Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (3): 522-529.DOI: 10.3969/j.issn.1000-2561.2019.03.015

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Biological Characteristics and Toxicity Test of Pathogen Strains Causing Rubber Red Root Rot from Different Regions

HE Chunping,LI Rui,LIANG Yanqiong,WU Weihuai,HUANG Xing,XI Jingen,ZHENG Jinlong,YI Kexian()   

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, China / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China
  • Received:2018-04-03 Revised:2019-01-04 Online:2019-03-15 Published:2019-03-15
  • Contact: LI Rui,YI Kexian

Abstract:

Red root disease of the rubber tree, which occurred widely, not only causes severe damage to rubber tree production, but also becomes one of the major obstacles to the sustainable development of the rubber tree industry in China. To clarify the biological characteristics and sensitivity to different fungicides, the mycelia growth of Ganoderma pseudoferreum was determined using the crossing method, and the inhibitory activities of 10 fungicides on G. pseudoferreum were also studied. The results showed that the mycelia of the pathogen grew better on the medium CMA with rubber root. They could grow at temperature of 13 to 31 ℃, best at 28 ℃. They grew well at pH varied from 3 to 10, best at 7–9. They could utilize many substances as carbon and nitrogen sources. Among carbon sources tested, fructose, galactose, maltose, glucose and mannose were found to be the best ones for the growth of G. pseudoferreum. Tyrosine and beef extract were the most suitable nitrogen sources while tryptophan and ureal were the worst. Darkness was good for mycelial growth while light had an inhibitory action. The lethal temperature for the mycelium was 47 ℃ for 10 min. The sensitivity of the isolates sourced from different regions to fungicides was different from each other. Tebuconazole was the most effective fungicide against the pathogen with an EC50 of 0.0312 μg/mL, followed by azoxystrobin, tridemorph, prochloraz and mycolbutanil with an EC50 of 0.5581, 0.6759, 1.3763 and 1.5603 μg/mL respectively.

Key words: rubber red root rot, Ganoderma pseudoferreum, biological characteristics, toxicity