Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (3): 483-489.DOI: 10.3969/j.issn.1000-2561.2019.03.010
• Biotechnology and Tissue Culture • Previous Articles Next Articles
WU Yuanhang1,LIU Qin1,LIU Pandao2,GUO Pengfei1,LI Min1,JIANG Lingyan1,LUO Lijuan1,*()
Received:
2018-06-05
Revised:
2019-01-09
Online:
2019-03-15
Published:
2019-03-15
Contact:
LUO Lijuan
WU Yuanhang,LIU Qin,LIU Pandao,GUO Pengfei,LI Min,JIANG Lingyan,LUO Lijuan. Cloning of Cassava Phenylalanine Ammonia Lyase Genes and Their Responses to Low Temperature Stress[J]. Chinese Journal of Tropical Crops, 2019, 40(3): 483-489.
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引物名称 Primer name | 正向引物序列 Forward sequence ( 5'-3' ) | 反向引物序列 Reverse sequence ( 5'-3' ) |
---|---|---|
MePAL1全长引物 | ATGGAAGTGTCCGGCGGAA | CTAACAGATTGGCAGAGGTGTG |
MePAL2全长引物 | ATGGACTTCTGTCACGACAA | TTAGGAGATGGGAAGAGGAGC |
MePAL3全长引物 | ATGGCAACAATCTCTCAGAATGG | TTAACATATTGGAAGAGGGGCAC |
MePAL4全长引物 | ATGGCAACAGTCTCTCAGAAT | TTAACAGATTGGAAGAGGGGCA |
MePAL5全长引物 | ATGGAGTTTTGTGAGGCTCACAAT | TTAGCAGATTGGAAGAGGAGCA |
MePAL6全长引物 | ATGGCTGCAAATGGCGTTG | TTAAAACAGAGGAGCTCTCTCTTTC |
MePAL1-定量引物 | ATCCTTTGCAGAAGCCTAAGCAG | TAATGCCAATCTAGCGTTGTCC |
MePAL2-定量引物 | AGCCTAGACAAGATCGTTATGCTC | TTGTGCAAACATGAGTTTCCC |
MePAL3-定量引物 | CTTATCAGCTATTTTCGCCGAAG | TCAATCTGAGGACCTAGCCAT |
MePAL4-定量引物 | GCACACAGTGAAGAACACCGTA | TAAGGCATGATCCACAAGCAC |
MePAL5-定量引物 | GCTCCAATTTCTTGCCAATCCTG | GCCGTTGATGCCCATTGTCA |
MePAL6-定量引物 | TGAATATCTCTCGTGGGACT | AGAATCTCCCACCGAATACCAG |
ef1α-内参引物 | TCCACTGGGTAGATTCGCTGTTA | TAGCTCCACTTGGGTCCTTCTT |
Tab. 1 The sequences of primers used in this study
引物名称 Primer name | 正向引物序列 Forward sequence ( 5'-3' ) | 反向引物序列 Reverse sequence ( 5'-3' ) |
---|---|---|
MePAL1全长引物 | ATGGAAGTGTCCGGCGGAA | CTAACAGATTGGCAGAGGTGTG |
MePAL2全长引物 | ATGGACTTCTGTCACGACAA | TTAGGAGATGGGAAGAGGAGC |
MePAL3全长引物 | ATGGCAACAATCTCTCAGAATGG | TTAACATATTGGAAGAGGGGCAC |
MePAL4全长引物 | ATGGCAACAGTCTCTCAGAAT | TTAACAGATTGGAAGAGGGGCA |
MePAL5全长引物 | ATGGAGTTTTGTGAGGCTCACAAT | TTAGCAGATTGGAAGAGGAGCA |
MePAL6全长引物 | ATGGCTGCAAATGGCGTTG | TTAAAACAGAGGAGCTCTCTCTTTC |
MePAL1-定量引物 | ATCCTTTGCAGAAGCCTAAGCAG | TAATGCCAATCTAGCGTTGTCC |
MePAL2-定量引物 | AGCCTAGACAAGATCGTTATGCTC | TTGTGCAAACATGAGTTTCCC |
MePAL3-定量引物 | CTTATCAGCTATTTTCGCCGAAG | TCAATCTGAGGACCTAGCCAT |
MePAL4-定量引物 | GCACACAGTGAAGAACACCGTA | TAAGGCATGATCCACAAGCAC |
MePAL5-定量引物 | GCTCCAATTTCTTGCCAATCCTG | GCCGTTGATGCCCATTGTCA |
MePAL6-定量引物 | TGAATATCTCTCGTGGGACT | AGAATCTCCCACCGAATACCAG |
ef1α-内参引物 | TCCACTGGGTAGATTCGCTGTTA | TAGCTCCACTTGGGTCCTTCTT |
基因名称 Gene name | 基因ID Gene ID | 编码区长度 CDS/bp | 蛋白 Protein | ||
---|---|---|---|---|---|
长度 Length/aa | 分子量 MW/ku | 等电点 pI | |||
MePAL1 | Manes.04G018000 | 2148 | 715 | 77.77 | 6.06 |
MePAL2 | Manes.07G098700 | 2112 | 703 | 76.97 | 6.00 |
MePAL3 | Manes.08G008400 | 2139 | 712 | 77.43 | 6.31 |
MePAL4 | Manes.09G063700 | 2151 | 716 | 77.71 | 5.90 |
MePAL5 | Manes.10G047500 | 2133 | 710 | 77.59 | 6.03 |
MePAL6 | Manes.16G098200 | 2136 | 711 | 78.16 | 5.98 |
Tab. 2 Characterization of the 6 PAL genes in cassava
基因名称 Gene name | 基因ID Gene ID | 编码区长度 CDS/bp | 蛋白 Protein | ||
---|---|---|---|---|---|
长度 Length/aa | 分子量 MW/ku | 等电点 pI | |||
MePAL1 | Manes.04G018000 | 2148 | 715 | 77.77 | 6.06 |
MePAL2 | Manes.07G098700 | 2112 | 703 | 76.97 | 6.00 |
MePAL3 | Manes.08G008400 | 2139 | 712 | 77.43 | 6.31 |
MePAL4 | Manes.09G063700 | 2151 | 716 | 77.71 | 5.90 |
MePAL5 | Manes.10G047500 | 2133 | 710 | 77.59 | 6.03 |
MePAL6 | Manes.16G098200 | 2136 | 711 | 78.16 | 5.98 |
Fig. 4 Changes in PAL activity, total phenolic content, flavonoid content and total antioxidant capacity in leaves of cassava KU50 under low temperature stress Asterisks indicate signifcant differences between independent samples, student t test (*: p<0.05, **: p<0.01,***: p<0.001).
Fig. 5 Expression of six PAL genes in cassava under low temperature stress Asterisks indicate signifcant differences between independent samples, student t test (*: p<0.05, **: p<0.01,***: p<0.001).
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