Study on Volatile Components of Croton crassifolius Leaf by HS- SPME-GC-MS

doi:10.3969/j.issn.1000-2561.2019.02.024

Abstract

Abstract:

The work was carried out to analyze the volatile components of Croton crassifolius Geisel. leaf by HS-SPME-GC-MS method. Based on the optimization for HS-SPME conditions, fifty-four compounds were identified by GC-MS and their relative contents were also calculated by area normalization method. It was found that the main volatile components of Croton crassifolius Geisel. leaf were (E)-2-Hexenal (38.62%), Linalool (11.96%) and Caryophyllene (11.10%), trans-Nerolidol (6.78%) and β-Elemene (3.67%), etc. Among these components, aldehyde, terpene and alcohol compounds accounted for 40.37%, 36.03%, and 21.90%, respectively, as well as small quantities of ester and ketone constituents.

Key words: Croton crassifolius Geisel. leaf, volatile components, HS-SPME, GC-MS

CLC Number:

R284. 1

ZHOU Xianglu,LYU Zhencheng,XU Liangxiong,PENG Yonghong. Study on Volatile Components of Croton crassifolius Leaf by HS- SPME-GC-MS[J]. Chinese Journal of Tropical Crops, 2019, 40(2): 380-387.

Figures/Tables 8

References 39

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序号 No.	化合物名称 Compound name	保留时间 Retention time/min	分子式 Molecular formula	相对百分含量 Relative content/%
1	己醛Hexanal	3.912	C₆H₁₂O	1.50
2	反-2-己烯醛(E)-2-Hexenal	5.116	C₆H₁₀O	38.62
3	2-乙基呋喃Furan, 2-ethyl	6.911	C₆H₈O	0.49
4	5-乙基-2-(5H)呋喃酮2(5H)-Furanone, 5-ethyl	8.421	C₆H₈O₂	0.36
5	β-蒎烯β-Pinene	9.044	C₁₀H₁₆	0.53
6	β-月桂烯β-Myrcene	9.692	C₁₀H₁₆	0.46
7	1,2-二甲基-1,5-环辛二烯1,2-Dimethyl-1,5-cyclooctadiene	10.201	C₁₀H₁₆	0.12
8	桉树醇Eucalyptol	11.057	C₁₀H₁₈O	0.72
9	3-蒈烯3-Carene	11.747	C₁₀H₁₆	2.16
10	蒿醇Artemisol	12.126	C₁₀H₁₈O	0.37
11	1-壬烯-3-醇1-Nonen-3-ol	12.946	C₉H₁₈O	0.88
12	3-壬酮3-Nonanone	13.236	C₉H₁₈O	0.46
13	芳樟醇Linalool	13.734	C₁₀H₁₈O	11.96
14	壬醛Nonanal	13.864	C₉H₁₈O	0.12
15	别罗勒稀Alloocimene	14.824	C₁₀H₁₆	0.16
16	α-松油醇α-Terpineol	17.065	C₁₀H₁₈O	0.41
17	癸醛Decanal	17.621	C₁₀H₂₀O	0.13
18	2-(3-甲基-2-烯-1-丁基）环戊酮 2-(3-methylbut-2-en-1-yl)cyclopentanone	18.181	C₁₀H₁₆O	0.15
19	6,6-二甲基二环[3.1.1]庚-2-基乙酸甲酯 (6,6-dimethylbicyclo[3.1.1]heptan-2-yl)methyl acetate	19.426	C₁₂H₂₀O₂	0.16
20	左旋乙酸龙脑酯l-Bornyl acetate	20.485	C₁₂H₂₀O₂	0.15
21	α-荜澄茄油烯α-Cubebene	23.105	C₁₅H₂₄	0.53
22	α-古巴烯α-Copaene	24.272	C₁₅H₂₄	0.23
23	4,4,6-三甲基-1-亚甲基-4-乙烯基二环[4.3.0]壬烷 4,6,6-trimethyl-1-methylene-4-vinyloctahydro-1H-indene	24.698	C₁₅H₂₄	0.22
24	β-榄香烯β-Elemene	25.040	C₁₅H₂₄	3.67
25	石竹烯Caryophyllene	26.332	C₁₅H₂₄	11.10
26	β-依兰烯β-Ylangene	26.727	C₁₅H₂₄	0.18
27	双环大根香叶烯Bicyclogermacrene	26.960	C₁₅H₂₄	2.02
28	α-香柑油烯α.-Bergamotene	27.064	C₁₅H₂₄	0.46
29	γ-依兰油烯γ-Muurolene	27.681	C₁₅H₂₄	0.19
30	（Z,Z,Z）-1,5,9,9-四甲基-1,4,7-环十一碳三烯 (1Z,4Z,7Z)-1,5,9,9-tetramethylcycloundeca-1,4,7-triene	27.899	C₁₅H₂₄	2.60
31	(E)-β-金合欢烯(E)-β-Famesene	28.112	C₁₅H₂₄	0.57
32	二环倍半水芹烯(+)-epi-Bicyclosesquiphellandrene	28.351	C₁₅H₂₄	0.13
33	白菖烯Calarene	29.036	C₁₅H₂₄	0.41
34	(1R,2S,6S,7S,8S)-1甲基-3-亚甲基-异丙基-三环- [4.4.0.0^2,7]-葵烷(1R,2S,6S,7S,8S)-8-isopropyl-1-methyl-3- methylenetricyclo[4.4.0.0^2,7]decane	29.222	C₁₅H₂₄	0.20
35	β-瑟林烯β-Selinene	29.482	C₁₅H₂₄	1.23
36	γ-古芸烯γ-Gurjunene	29.638	C₁₅H₂₄	0.32
37	γ-马榄烯γ-Maaliene	29.840	C₁₅H₂₄	2.93
序号 No.	化合物名称 Compound name	保留时间 Retention time/min	分子式 Molecular formula	相对百分含量 Relative content/%
38	α-衣兰油烯α-Muurolene	30.208	C₁₅H₂₄	0.26
39	异丁子香烯Isocaryophillene	30.655	C₁₅H₂₄	0.21
40	γ-杜松烯 γ-Cadinene	30.872	C₁₅H₂₄	0.17
41	α-马榄烯α-Maaliene	31.023	C₁₅H₂₄	0.64
42	(1S,8aR)-1-异丙基-4,7-二甲基-1,2,3,5,6,8a-六氢萘 (1S,8aR)-1-isopropyl-4,7-dimethyl- 1,2,3,5,6,8a-hexahydronaphthalene	31.360	C₁₅H₂₄	0.61
43	α-柏木烯α-Cedrene	31.760	C₁₅H₂₄	0.21
44	巴伦西亚橘烯Valencene	31.879	C₁₅H₂₄	0.28
45	β-马榄烯β-Maaliene	32.061	C₁₅H₂₄	1.24
46	芹子-3,7(11)-二烯Selina-3,7(11)-diene	32.206	C₁₅H₂₄	0.27
47	榄香醇Elemol	32.621	C₁₅H₂₆O	0.18
48	β-大根香叶烯β-Germacrene	32.953	C₁₅H₂₄	0.13
49	反-橙花叔醇trans-Nerolidol	33.488	C₁₅H₂₆O	6.78
50	氧化石竹烯Caryophyllene oxide	34.266	C₁₅H₂₄O	0.44
51	3,5,11-桉叶烷三烯3,5,11-Eudesmatriene	36.030	C₁₅H₂₂	0.20
52	木香醇Costol	38.723	C₁₅H₂₄O	0.19
53	(1R,4aR)-1,4a二甲基-7-(1-甲基亚乙基)-十氢萘醇 (1R,4aR)-1,4a-dimethyl-7-(propan-2-ylidene)decahydronaphthalen-1-ol	39.927	C₁₅H₂₆O	0.25
54	(E)-6,10-二甲基-2-(4-甲基苯基)-5-十一烯 (E)-1-(6,10-dimethylundec-5-en-2-yl)-4-methylbenzene	51.056	C₂₀H₃₂	0.26

序号 No.	化合物名称 Compound name	保留时间 Retention time/min	分子式 Molecular formula	相对百分含量 Relative content/%
1	己醛Hexanal	3.912	C₆H₁₂O	1.50
2	反-2-己烯醛(E)-2-Hexenal	5.116	C₆H₁₀O	38.62
3	2-乙基呋喃Furan, 2-ethyl	6.911	C₆H₈O	0.49
4	5-乙基-2-(5H)呋喃酮2(5H)-Furanone, 5-ethyl	8.421	C₆H₈O₂	0.36
5	β-蒎烯β-Pinene	9.044	C₁₀H₁₆	0.53
6	β-月桂烯β-Myrcene	9.692	C₁₀H₁₆	0.46
7	1,2-二甲基-1,5-环辛二烯1,2-Dimethyl-1,5-cyclooctadiene	10.201	C₁₀H₁₆	0.12
8	桉树醇Eucalyptol	11.057	C₁₀H₁₈O	0.72
9	3-蒈烯3-Carene	11.747	C₁₀H₁₆	2.16
10	蒿醇Artemisol	12.126	C₁₀H₁₈O	0.37
11	1-壬烯-3-醇1-Nonen-3-ol	12.946	C₉H₁₈O	0.88
12	3-壬酮3-Nonanone	13.236	C₉H₁₈O	0.46
13	芳樟醇Linalool	13.734	C₁₀H₁₈O	11.96
14	壬醛Nonanal	13.864	C₉H₁₈O	0.12
15	别罗勒稀Alloocimene	14.824	C₁₀H₁₆	0.16
16	α-松油醇α-Terpineol	17.065	C₁₀H₁₈O	0.41
17	癸醛Decanal	17.621	C₁₀H₂₀O	0.13
18	2-(3-甲基-2-烯-1-丁基）环戊酮 2-(3-methylbut-2-en-1-yl)cyclopentanone	18.181	C₁₀H₁₆O	0.15
19	6,6-二甲基二环[3.1.1]庚-2-基乙酸甲酯 (6,6-dimethylbicyclo[3.1.1]heptan-2-yl)methyl acetate	19.426	C₁₂H₂₀O₂	0.16
20	左旋乙酸龙脑酯l-Bornyl acetate	20.485	C₁₂H₂₀O₂	0.15
21	α-荜澄茄油烯α-Cubebene	23.105	C₁₅H₂₄	0.53
22	α-古巴烯α-Copaene	24.272	C₁₅H₂₄	0.23
23	4,4,6-三甲基-1-亚甲基-4-乙烯基二环[4.3.0]壬烷 4,6,6-trimethyl-1-methylene-4-vinyloctahydro-1H-indene	24.698	C₁₅H₂₄	0.22
24	β-榄香烯β-Elemene	25.040	C₁₅H₂₄	3.67
25	石竹烯Caryophyllene	26.332	C₁₅H₂₄	11.10
26	β-依兰烯β-Ylangene	26.727	C₁₅H₂₄	0.18
27	双环大根香叶烯Bicyclogermacrene	26.960	C₁₅H₂₄	2.02
28	α-香柑油烯α.-Bergamotene	27.064	C₁₅H₂₄	0.46
29	γ-依兰油烯γ-Muurolene	27.681	C₁₅H₂₄	0.19
30	（Z,Z,Z）-1,5,9,9-四甲基-1,4,7-环十一碳三烯 (1Z,4Z,7Z)-1,5,9,9-tetramethylcycloundeca-1,4,7-triene	27.899	C₁₅H₂₄	2.60
31	(E)-β-金合欢烯(E)-β-Famesene	28.112	C₁₅H₂₄	0.57
32	二环倍半水芹烯(+)-epi-Bicyclosesquiphellandrene	28.351	C₁₅H₂₄	0.13
33	白菖烯Calarene	29.036	C₁₅H₂₄	0.41
34	(1R,2S,6S,7S,8S)-1甲基-3-亚甲基-异丙基-三环- [4.4.0.0^2,7]-葵烷(1R,2S,6S,7S,8S)-8-isopropyl-1-methyl-3- methylenetricyclo[4.4.0.0^2,7]decane	29.222	C₁₅H₂₄	0.20
35	β-瑟林烯β-Selinene	29.482	C₁₅H₂₄	1.23
36	γ-古芸烯γ-Gurjunene	29.638	C₁₅H₂₄	0.32
37	γ-马榄烯γ-Maaliene	29.840	C₁₅H₂₄	2.93
序号 No.	化合物名称 Compound name	保留时间 Retention time/min	分子式 Molecular formula	相对百分含量 Relative content/%
38	α-衣兰油烯α-Muurolene	30.208	C₁₅H₂₄	0.26
39	异丁子香烯Isocaryophillene	30.655	C₁₅H₂₄	0.21
40	γ-杜松烯 γ-Cadinene	30.872	C₁₅H₂₄	0.17
41	α-马榄烯α-Maaliene	31.023	C₁₅H₂₄	0.64
42	(1S,8aR)-1-异丙基-4,7-二甲基-1,2,3,5,6,8a-六氢萘 (1S,8aR)-1-isopropyl-4,7-dimethyl- 1,2,3,5,6,8a-hexahydronaphthalene	31.360	C₁₅H₂₄	0.61
43	α-柏木烯α-Cedrene	31.760	C₁₅H₂₄	0.21
44	巴伦西亚橘烯Valencene	31.879	C₁₅H₂₄	0.28
45	β-马榄烯β-Maaliene	32.061	C₁₅H₂₄	1.24
46	芹子-3,7(11)-二烯Selina-3,7(11)-diene	32.206	C₁₅H₂₄	0.27
47	榄香醇Elemol	32.621	C₁₅H₂₆O	0.18
48	β-大根香叶烯β-Germacrene	32.953	C₁₅H₂₄	0.13
49	反-橙花叔醇trans-Nerolidol	33.488	C₁₅H₂₆O	6.78
50	氧化石竹烯Caryophyllene oxide	34.266	C₁₅H₂₄O	0.44
51	3,5,11-桉叶烷三烯3,5,11-Eudesmatriene	36.030	C₁₅H₂₂	0.20
52	木香醇Costol	38.723	C₁₅H₂₄O	0.19
53	(1R,4aR)-1,4a二甲基-7-(1-甲基亚乙基)-十氢萘醇 (1R,4aR)-1,4a-dimethyl-7-(propan-2-ylidene)decahydronaphthalen-1-ol	39.927	C₁₅H₂₆O	0.25
54	(E)-6,10-二甲基-2-(4-甲基苯基)-5-十一烯 (E)-1-(6,10-dimethylundec-5-en-2-yl)-4-methylbenzene	51.056	C₂₀H₃₂	0.26