Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 300-307.DOI: 10.3969/j.issn.1000-2561.2019.02.013

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Genetic Diversity of Coffee Germplasms by ISSR Markers

YAN Lin1,HUANG Lifang1,WANG Xiaoyang1,ZHOU Hua2,CHENG Jinhuan3,LI Jinhong2,LONG Yuzhou1,DONG Yunping1,*()   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agriculturol Sciences / Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural affairs / Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
    2. Dehong Tropical Agriculture Institute of Yunnan, Ruili, Yunnan 678600, China
    3. Tropical and Subtropical Economic Crops Research Institute, Yunnan Academy of Agricultural Sciences, Baoshan, Yunnan 678025, China
  • Received:2018-04-18 Revised:2018-10-28 Online:2019-02-25 Published:2019-05-16
  • Contact: DONG Yunping


To assess the genetic diversity and the relationship of coffee germplasm accessions, 87 resources were analyzed by ISSR molecular marker technology. The results demonstrated that 140 bands were amjplified by 19 primers, among which 107 bands were polymorphic, accounting for 76.4%. Based on a clustering graph using UPGMA, 87 resources could be divided into three groups at the level of 0.625. Three accessions in C. liberica were in the first group. One accessions in C. canephora was in the second group. A total of 83 accessions in C. arabica were in the third group. There were large genetic differences among species (C. arabica, C. canephora, and C. liberica), and the small genetic differences within Coffea arabica. These results would provide an important scientific basis for pineapple germplasm identification, classification and molecular breeding of coffee. The ISSR markers was an available method to study the genetic diversity of coffee germplasm resources.

Key words: coffee, ISSR, cluster, genetic diversity

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