Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 281-288.DOI: 10.3969/j.issn.1000-2561.2019.02.011

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Gene Cloning and Expression Analysis of the COP9 Signalosome Members in Laticifer Cells of Rubber Tree

WU Shaohua,ZHANG Shixin,DENG Xiaomin,CHEN Yueyi,TIAN Weimin()   

  1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Haikou, Hainan 571101, China
  • Received:2018-07-19 Revised:2018-09-14 Online:2019-02-25 Published:2019-05-16
  • Contact: TIAN Weimin

Abstract:

The COP9 signalosome (CSN) is an evolutionarily conservative protein complex and plays a crucial role in jasmonate (JA) signaling. The laticifer in rubber tree is specific for rubber biosynthesis. Although it is suggested that rubber biosynthesis in laticifer may be regulated by JA signaling, little is known about the regulatory mechanism of the JA signaling in rubber biosynthesis. In this study, the full-length cDNAs of eight CSN genes (designated as HbCSN1 to HbCSN8) which were respectively related to the eight CSN genes in Arabidopsis were cloned from the laticifer of rubber trees. The differentially expressed pattern of the eight CSN genes among bark tissues, leaves at the different development stages, latex, male and female flowers was revealed by real-time quantitative RT-PCR. The result showed that the eight HbCSNs were differentially expressed in all the tested tissues. Of which, HbCSN5 was the most abundance in latex, and the other HbCSNs were mainly expressed in the leaf. Additionally, most of the eight CSN genes in laticifer were up-regulated by tapping and methyl jasmonate (MeJA). The differentially responsive CSN members indicated that the COP9 signalosome may be involved in the JA signalings of latex in rubber trees.

Key words: Hevea brasiliensis Muell. Arg., COP9 signalosome, gene cloning and expression, phylogenic analysis

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