Chemical Mutagenesis for the Cuttings of <i>Bougainvillea glabra</i> ‘Mrs. Eva’ and <i>B.</i> × <i>buttiana</i> ‘Miss Manila’
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (2): 238-246.DOI: 10.3969/j.issn.1000-2561.2019.02.005

• Crop Culture and Nutrition, Genetic Breeding • Previous Articles     Next Articles

Chemical Mutagenesis for the Cuttings of Bougainvillea glabra ‘Mrs. Eva’ and B. × buttiana ‘Miss Manila’

CHANG Shengxin1,2,HUANG Surong1,2,XU Shisong1,2,YANG Guangsui1,2,*()   

  1. 1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
    2. Hainan Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, Hainan 571737, China
  • Received:2018-05-27 Revised:2018-09-26 Online:2019-02-25 Published:2019-05-16
  • Contact: YANG Guangsui

Abstract:

Bougainvillea Comm. ex Juss is an important ornamental plant in the tropical and sub-tropical regions of China. Because the sexual reproduction of Bougainvillea is difficult, mutation breeding is an available breeding method. The present study used ethylmethane sulfonate (EMS), sodium azide (NaN3) and pingyangmycin (PYM) to mutagenize the cuttings of B. glabra ‘Mrs. Eva’ and B. × buttiana ‘Miss Manila’, respectively. In 20 h EMS treatments, the half lethal dose of ‘Mrs. Eva’ and ‘Miss Manila’ cuttings was 0.22% and 0.62%, respectively. With the increasing of EMS concentration, the shoot number and length of the longest branch of two Bougainvillea varieties showed a decreasing trend, while the chlorophyll and carotenoid content increased gradually. With the increasing of EMS concentration, the leaf hue of ‘Mrs. Eva’ changed to grayish tone and that of ‘Miss Manila’ changed to red tone gradually. Almost all ‘Mrs. Eva’ cuttings died in 20 h NaN3 treatments, while the survival rate of ‘Miss Manila’ cuttings was 82%-92%. With the increasing of NaN3 concentration, the shoot number of ‘Miss Manila’ decreased significantly. 20 h PYM treatments significantly improved the length of the longest branch of two Bougainvillea varieties in contrast to the control, but had no significant influence on the survival rate. Moreover, variant seedlings were found in EMS and PYM treatments, representing transformative leaf margin, wrinkled leaf surface or double leaf tips.

Key words: Bougainvillea, ethylmethane sulfonate, sodium azide, pingyangmycin

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