Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 199-207.DOI: 10.3969/j.issn.1000-2561.2019.01.029

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Research Progress on DNA Methylation During Plant Tissue Culture

SHI Peng,WANG Yong,JIN Longfei,ZHANG Dapeng,ZHAO Zhihao,CAO Hongxing,LEI Xintao()   

  1. Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Biological Laboratory of Tropical Oil Crops, Wenchang, Hainan 571339, China
  • Received:2018-07-13 Revised:2018-09-04 Online:2019-01-15 Published:2019-01-15
  • Contact: LEI Xintao


Tissue culture is the important technical basis for plant asexual propagation and genetic transformation. Tissue culture includes explants dedifferentiation, callus formation, callus redifferentiation and plantlets formation. DNA methylation pattern is constantly changing during tissue culture, which means many epigenetic reprogramming events occur in cells. Generally speaking, DNA methylation can promote or accelerate the tissue culture process, it’s very important for many plants that are difficult to regenerate. The paper mainly summarizes the pattern change and molecular mechanism of DNA methylation during plant tissue culture, as well as new methods and ideas. We hope the paper could help the study of DNA methylation in plant tissue culture.

Key words: plant, tissue culture, DNA methylation