Optimization for Enzymatic Extraction Process of Polysaccharide from <i>Moringa oleifera</i> Lam. and the Contents
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 158-165.DOI: 10.3969/j.issn.1000-2561.2019.01.023

• Agricultural Product Processing, Preservation, Storage, Analysis and Detection • Previous Articles     Next Articles

Optimization for Enzymatic Extraction Process of Polysaccharide from Moringa oleifera Lam. and the Contents

ZHOU Danrong1,YE Xinfu1,*,WANG Xiao’an1,WEI Xiaoxia1,LIU Ping2   

  1. 1. Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
    2. Yangtze University, Jingzhou, Hubei 434023, China
  • Received:2018-04-10 Revised:2018-06-07 Online:2019-01-15 Published:2019-01-15
  • Contact: YE Xinfu


Single factor experiments incluing enzymatic dosage, extraction temperature, extraction time, ratio of liquid to material, and quadratic regression orthogonal rotation combination design were used to optimize the enzymatic extraction process of polysaccharide from Moringa oleifera Lam.. The quadratic orthogonal regression model of extracted polysaccharide rate (Y) for enzymatic dosage (X1), extraction temperature (X2), extraction time (X3) and ratio of liquid to material (X4) was Y=18.6602+0.8134X1+0.7572X2-0.4312X3+0.6909X4-0.5181X1 2-0.4935X2 2-0.6277X4 2, which had a good fitting degree. The four factors had significant effects on rate of polysaccharide extracted (p<0.05). Furthermore, the optimal compound enzymatic extraction process conditions were as follows: enzymatic dosage of 1.60%, extraction temperature of 53 ℃, extraction time of 68 min, and ratio of liquid to material of 52∶1. Under optimal conditions, the verified value of extracted polysaccharide rate was 19.83%. The contents of polysaccharide from different parts of Moringa had remarkable difference. The content was ranked downward in root, flower, tender leaves, and stalk. It was accurate and reliable that the quadratic regression orthogonal rotation combination design was used to optimize enzymatic extraction process conditions,and the extraction efficiency of polysaccharide from Moringa could be improved effectively, so it could be applied in the actual production. Furthermore, the content of polysaccharide in the root of Moringa was the highest and it was worthy of exploitation and utilization.

Key words: Moringa oleifera Lam., polysaccharide, cello lase, orthogonal rotation combination design, content