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Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 107-114.DOI: 10.3969/j.issn.1000-2561.2019.01.016
• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles Next Articles
WU Ruhui,LI Zengping(
),ZHANG Yu,SHI Zekun
Received:2018-07-11
Revised:2018-08-20
Online:2019-01-15
Published:2019-01-15
Contact:
LI Zengping
WU Ruhui,LI Zengping,ZHANG Yu,SHI Zekun. Identification and Biological Characteristics of the Pathogen Causing Lasiodiplodia Leaf Spot of Robber Tree[J]. Chinese Journal of Tropical Crops, 2019, 40(1): 107-114.
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Fig. 1 Field symptoms of Lasiodiplodia leaf spot on rubber tree A: Initial symptoms; B: Round and semicircular lesions on the leaf.
Fig. 2 Pathogenicity test of Lasiodiplodia leaf spot on rubber tree A: Symptoms after 3 days of leaf inoculation; B: Symptoms after 7 days of leaf inoculation; C: Symptoms after 3 days of stem inoculation; D, E: Symptoms after 14 days of stem inoculation.
Fig. 3 The pathogen of Lasiodiplodia leaf spot on rubber tree A: Pathogen’s colony morphology on PDA medium (2 d); B: Pycnidia produced on diseased leaves; C: Pycnidia produced on the tender stems of the disease; D: Pycnidia aggregation; E: Agglomerated pycnidia; F: Timmature conidia; G: Mature conidia.
Fig. 4 Phylogenetic tree of isolate HNSY003 and species of Lasiodiplodia based on combined rDNA-ITS and EF1-α gene sequences
Fig. 5 The mycelia growth with different temperatures Different capital letters mean extremely significant different at 0.01 level; different lowercase letters mean significant different at 0.05 level.
Fig. 6 The mycelia growth with different light conditions Different capital letters mean extremely significant difference at 0.01 level; different lowercase letters mean significant difference at 0.05 level.
Fig. 7 The mycelia growth under different pH conditions Different capital letters mean extremely significant difference at 0.01 level; different lowercase letters mean significant difference at 0.05 level.
| 碳源Carbon source | 菌落直径Colony diameter/cm | 菌落特征Colony characteristics |
|---|---|---|
| D-果糖D-Fructose | 7.38±0.03aA | 菌落圆形,菌丝絮状,气生菌丝发达,致密 |
| 可溶性淀粉Soluble starch | 5.93±0.03bB | 菌落圆形,菌丝白色,较薄 |
| 葡萄糖Glucose | 5.62±0.03cC | 菌落圆形,菌丝絮状,致密 |
| 麦芽糖Maltcose | 5.57±0.08cC | 菌落圆形,菌丝絮状,稀薄 |
| 蔗糖Sucrose | 5.55±0.01cC | 菌落圆形,菌丝絮状,较密 |
| 肌醇Inositol | 5.05±0.10dD | 菌落圆形,丝絮状,白色,菌丝稀薄 |
| D-山梨醇D-Sorbitol | 4.83±0.03eE | 菌落圆形,菌丝絮状,乳白色,较薄 |
| 对照CK | 3.78±0.06fF | 菌落圆形,菌丝白色,稀薄 |
Tab. 1 Effect of different carbon sources on mycelia growth
| 碳源Carbon source | 菌落直径Colony diameter/cm | 菌落特征Colony characteristics |
|---|---|---|
| D-果糖D-Fructose | 7.38±0.03aA | 菌落圆形,菌丝絮状,气生菌丝发达,致密 |
| 可溶性淀粉Soluble starch | 5.93±0.03bB | 菌落圆形,菌丝白色,较薄 |
| 葡萄糖Glucose | 5.62±0.03cC | 菌落圆形,菌丝絮状,致密 |
| 麦芽糖Maltcose | 5.57±0.08cC | 菌落圆形,菌丝絮状,稀薄 |
| 蔗糖Sucrose | 5.55±0.01cC | 菌落圆形,菌丝絮状,较密 |
| 肌醇Inositol | 5.05±0.10dD | 菌落圆形,丝絮状,白色,菌丝稀薄 |
| D-山梨醇D-Sorbitol | 4.83±0.03eE | 菌落圆形,菌丝絮状,乳白色,较薄 |
| 对照CK | 3.78±0.06fF | 菌落圆形,菌丝白色,稀薄 |
| 氮源Nitrogen source | 菌落直径Colony diameter/cm | 菌落特征Colony characteristics |
|---|---|---|
| 酵母浸膏Yeast extract | 6.82±0.06aA | 菌落圆形,致密,菌丝絮状,气生菌丝发达 |
| 大豆蛋白胨Soya peptone | 6.12±0.06bB | 菌落圆形,致密,菌丝絮状,气生菌丝发达 |
| L-天冬酰胺L-Asparagine | 5.68±0.03cC | 菌落近圆形,菌丝絮状,气生菌丝发达,较密 |
| 牛肉浸膏Beef extract | 5.38±0.01dD | 菌落圆形,菌丝絮状,气生菌丝发达,较密 |
| 硝酸铵NH4NO3 | 5.27±0.03eE | 菌落近圆形,菌丝絮状,气生菌丝发达,较密 |
| 硝酸钙Ca(NO3)2 | 5.22±0.03fF | 菌落圆形,白色,致密 |
| 硝酸钾KNO3 | 5.18±0.03fF | 菌落圆形,米白色,气生菌丝发达 |
| 硫酸铵(NH4)2SO4 | 5.17±0.03fgFG | 菌落近圆形,白色,菌丝较密 |
| 硝酸钠NaNO3 | 5.07±0.03fgFG | 菌落近圆形,白色,菌丝稀疏 |
| 氯化铵NH4Cl | 5.02±0.03hH | 菌落近圆形,白色,菌丝稀疏 |
| 草酸铵(NH4)2C2O4 | 3.68±0.03iI | 菌落近圆形,白色,菌丝稀疏 |
| 对照CK | 5.07±0.03ghGH | 菌落圆形,菌丝稀薄,白色 |
Tab. 2 Effect of different nitrogen sources on mycelia growth
| 氮源Nitrogen source | 菌落直径Colony diameter/cm | 菌落特征Colony characteristics |
|---|---|---|
| 酵母浸膏Yeast extract | 6.82±0.06aA | 菌落圆形,致密,菌丝絮状,气生菌丝发达 |
| 大豆蛋白胨Soya peptone | 6.12±0.06bB | 菌落圆形,致密,菌丝絮状,气生菌丝发达 |
| L-天冬酰胺L-Asparagine | 5.68±0.03cC | 菌落近圆形,菌丝絮状,气生菌丝发达,较密 |
| 牛肉浸膏Beef extract | 5.38±0.01dD | 菌落圆形,菌丝絮状,气生菌丝发达,较密 |
| 硝酸铵NH4NO3 | 5.27±0.03eE | 菌落近圆形,菌丝絮状,气生菌丝发达,较密 |
| 硝酸钙Ca(NO3)2 | 5.22±0.03fF | 菌落圆形,白色,致密 |
| 硝酸钾KNO3 | 5.18±0.03fF | 菌落圆形,米白色,气生菌丝发达 |
| 硫酸铵(NH4)2SO4 | 5.17±0.03fgFG | 菌落近圆形,白色,菌丝较密 |
| 硝酸钠NaNO3 | 5.07±0.03fgFG | 菌落近圆形,白色,菌丝稀疏 |
| 氯化铵NH4Cl | 5.02±0.03hH | 菌落近圆形,白色,菌丝稀疏 |
| 草酸铵(NH4)2C2O4 | 3.68±0.03iI | 菌落近圆形,白色,菌丝稀疏 |
| 对照CK | 5.07±0.03ghGH | 菌落圆形,菌丝稀薄,白色 |
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