Identification and Biological Characteristics of the Pathogen Causing <i>Lasiodiplodia</i> Leaf Spot of Robber Tree
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 107-114.DOI: 10.3969/j.issn.1000-2561.2019.01.016

• Crop Pests, Diseases and Their Control, Environmental Protection • Previous Articles     Next Articles

Identification and Biological Characteristics of the Pathogen Causing Lasiodiplodia Leaf Spot of Robber Tree

WU Ruhui,LI Zengping(),ZHANG Yu,SHI Zekun   

  1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received:2018-07-11 Revised:2018-08-20 Online:2019-01-15 Published:2019-01-15
  • Contact: LI Zengping


A new leaf spot disease of rubber tree was found in Sanya, Hainan. The pathogen strain HNSY003 was isolated and purified by methods of tissue isolation and single spore isolation. The pathogen was identified as Lasiodiplodia pseudotheobromae by morphological observation, sequence analyses of rDNA-ITS and EF1-α, and its pathogenicity was confirmed by Koch’s postulate. The fungal species is reported the first time as the pathogen of robber tree leaf spot. The results of biological characteristics test showed that the optimum conditions for the pathogen growth were as follows: temperature 32 ℃, pH 5, D-fructose as the carbon source, yeast extract as the nitrogen source. Different light conditions had little effect on the growth rate of the pathogen hypha.

Key words: rubber tree, Lasiodiplodia leaf spot, Lasiodiplodia pseudotheobromae, biological characteristic