Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 98-106.DOI: 10.3969/j.issn.1000-2561.2019.01.015

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Tissue Culture and Plant Regeneration of Leaves of Primulina guigangensis

YAN Haixia,HUANG Changyan,ZHANG Zibin,CUI Xueqiang,DENG Jieling,GUAN Shikai,BU Zhaoyang()   

  1. Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China
  • Received:2018-05-16 Revised:2018-07-23 Online:2019-01-15 Published:2019-01-15
  • Contact: BU Zhaoyang


The effects of different media on the primary induction, adventitious buds proliferation, callus differentiation and rooting culture were investigated using the leaves of P. guigangensis as the explants. The results showed that the rip cutting was the suitable method; the suitable medium for adventitious buds regeneration was MS+6-BA 4.0 mg/L+IAA 1.5 mg/L; the suitable medium for callus induction was MS+6-BA 3.0-5.0 mg/L+2,4-D 0.5-1.5 mg/L; both rate of adventitious buds induction and rate of callus induction were 100.00%; the suitable medium for callus differentiation was MS+KT 1.0 mg/L+NAA 0.2 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L, and the differentiation coefficient was 12.64; the suitable medium for adventitious buds proliferation was MS+ZT 1.0 mg/L+NAA 0.10 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L, and the multiplication coefficient was 8.55; the suitable medium for rooting culture was 3/4 MS+NAA 0.01-0.05 mg/L+active carbon 1.0-3.0 g/L and the rooting rate was 100%. It was concluded that the leaves rip cutting could be used to establish the tissue culture system of P. guigangensis by the regeneration methods of adventitious buds and callus which had high induction and proliferation rates of adventitious buds and good rooting growth.

Key words: Primulina guigangensis, tissue culture, regeneration system, natural extracts