Rapid Propagation of Tillandsia cyanea ‘Bert’ Through Liquid Shake Culture

doi:10.3969/j.issn.1000-2561.2019.01.014

Abstract

Abstract:

Using the leaf of Tillandsia cyanea as the explants, the fragile callus was induced. The rapid multiplication of embryonic callus was realized through liquid shake culture. In solid medium, the germination of the embryos and rooting of adventitious shoots were induced respectively. Rooting plantlets were transplanted and survived with high rates. In the optimized culture condition, the induction rate of callus was more than 90%. All the embryonic callus turned into embryos after liquid culture and the induction rate of the explants volume reached 5.0. More than 90% embryos developed into adventitious buds and 95% of the buds rooted after culture in solid medium. The transplanting survival rate was also more than 95%. These results indicated that the liquid oscillation and rapid propagation of T. cyanea was successful.

Key words: Tillandsia cyanea ‘Bert’, embryonic callus, liquid shake culture, rapid propagation

LI Zhiying,FU Yunliu,XU Li. Rapid Propagation of Tillandsia cyanea ‘Bert’ Through Liquid Shake Culture[J]. Chinese Journal of Tropical Crops, 2019, 40(1): 92-97.

Figures/Tables 5

References 18

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6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	接种块数 Number of explants	出愈伤组织的块数 Number of callus	愈伤组织诱导率 Induction ratio/%
2.0	0.02	50	48	96
2.5	0.02	50	49	98
3.0	0.02	50	46	92

6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	接种块数 Number of explants	出愈伤组织的块数 Number of callus	愈伤组织诱导率 Induction ratio/%
2.0	0.02	50	48	96
2.5	0.02	50	49	98
3.0	0.02	50	46	92

2,4-D/ (mg·L^-1)	KT/ (mg·L^-1)	平均每代增殖体积Volume increment/mL	平均每个周期增殖系数Multiplication
0.1	0	—	—
0.3	0	—	—
0.5	0	—	—
0.1	0.01	2.9	3.0^b
0.1	0.03	3.1	3.3^b
0.1	0.05	3.1	3.3^b
0.3	0.01	3.2	3.5^bc
0.3	0.03	3.2	3.8^bc
0.3	0.05	3.7	5.0^c
0.5	0.01	1.2	1.4^a
0.5	0.03	1.3	1.4^a
0.5	0.05	1.4	1.6^a

2,4-D/ (mg·L^-1)	KT/ (mg·L^-1)	平均每代增殖体积Volume increment/mL	平均每个周期增殖系数Multiplication
0.1	0	—	—
0.3	0	—	—
0.5	0	—	—
0.1	0.01	2.9	3.0^b
0.1	0.03	3.1	3.3^b
0.1	0.05	3.1	3.3^b
0.3	0.01	3.2	3.5^bc
0.3	0.03	3.2	3.8^bc
0.3	0.05	3.7	5.0^c
0.5	0.01	1.2	1.4^a
0.5	0.03	1.3	1.4^a
0.5	0.05	1.4	1.6^a

6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	胚块萌发率 Germination rate of embryo piece/%	胚萌发率 Germination rate of embryos/%
0.1	0.05	30^a	11^a
0.5	0.05	50^b	23^ab
1.0	0.05	82^c	32^b
2.0	0.05	80^c	55^c
0.1	0.02	42^a	21^a
0.5	0.02	64^b	32^b
1.0	0.02	96^c	57^c
2.0	0.02	96^c	56^c
0.1	0.01	40^a	39^b
0.5	0.01	62^b	43^b
1.0	0.01	100^c	90^c
2.0	0.01	100^c	81^c