Rapid Propagation of <i>Tillandsia cyanea</i> ‘Bert’ Through Liquid Shake Culture
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 92-97.DOI: 10.3969/j.issn.1000-2561.2019.01.014

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Rapid Propagation of Tillandsia cyanea ‘Bert’ Through Liquid Shake Culture

LI Zhiying1,2,3,FU Yunliu1,2,3,XU Li1,2,3,*()   

  1. 1. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hannan 571737, China
    2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Danzhou, Hainan 571737, China
    3. Hainan Provincial Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, Danzhou, Hainan 571737, China
  • Received:2018-06-22 Revised:2018-11-03 Online:2019-01-15 Published:2019-01-15
  • Contact: XU Li


Using the leaf of Tillandsia cyanea as the explants, the fragile callus was induced. The rapid multiplication of embryonic callus was realized through liquid shake culture. In solid medium, the germination of the embryos and rooting of adventitious shoots were induced respectively. Rooting plantlets were transplanted and survived with high rates. In the optimized culture condition, the induction rate of callus was more than 90%. All the embryonic callus turned into embryos after liquid culture and the induction rate of the explants volume reached 5.0. More than 90% embryos developed into adventitious buds and 95% of the buds rooted after culture in solid medium. The transplanting survival rate was also more than 95%. These results indicated that the liquid oscillation and rapid propagation of T. cyanea was successful.

Key words: Tillandsia cyanea ‘Bert’, embryonic callus, liquid shake culture, rapid propagation