Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 92-97.DOI: 10.3969/j.issn.1000-2561.2019.01.014
• Biotechnology and Tissue Culture • Previous Articles Next Articles
LI Zhiying1,2,3,FU Yunliu1,2,3,XU Li1,2,3,*()
Received:
2018-06-22
Revised:
2018-11-03
Online:
2019-01-15
Published:
2019-01-15
Contact:
XU Li
LI Zhiying,FU Yunliu,XU Li. Rapid Propagation of Tillandsia cyanea ‘Bert’ Through Liquid Shake Culture[J]. Chinese Journal of Tropical Crops, 2019, 40(1): 92-97.
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Fig. 1 Regeneration of T. cyanea through callus induction from leaf and suspension of callus A: Embryonic callus induced from the base of in vitro leaf; B: Propagation of embryonic callus; C: Early stage of the suspension cultures; D: Embryo mass and embryos formed during the embryonic callus suspension culture; E: Form of the embryos; F: Malformed embryos; G: Adventitious bud developed from single embryo; H: Form of embryo mass after germination induction; I: Tufted adventitious buds developed from embryo mass; J: Separated bud; K: Rooted buds; L: Transplanted plantlets.
6-BA/ (mg·L-1) | NAA/ (mg·L-1) | 接种块数 Number of explants | 出愈伤组织的块数 Number of callus | 愈伤组织诱导率 Induction ratio/% |
---|---|---|---|---|
2.0 | 0.02 | 50 | 48 | 96 |
2.5 | 0.02 | 50 | 49 | 98 |
3.0 | 0.02 | 50 | 46 | 92 |
Tab. 1 Callus induction of T. cyanea from in vitro leaf
6-BA/ (mg·L-1) | NAA/ (mg·L-1) | 接种块数 Number of explants | 出愈伤组织的块数 Number of callus | 愈伤组织诱导率 Induction ratio/% |
---|---|---|---|---|
2.0 | 0.02 | 50 | 48 | 96 |
2.5 | 0.02 | 50 | 49 | 98 |
3.0 | 0.02 | 50 | 46 | 92 |
2,4-D/ (mg·L-1) | KT/ (mg·L-1) | 平均每代增殖体积Volume increment/mL | 平均每个周期增殖系数Multiplication |
---|---|---|---|
0.1 | 0 | — | — |
0.3 | 0 | — | — |
0.5 | 0 | — | — |
0.1 | 0.01 | 2.9 | 3.0b |
0.1 | 0.03 | 3.1 | 3.3b |
0.1 | 0.05 | 3.1 | 3.3b |
0.3 | 0.01 | 3.2 | 3.5bc |
0.3 | 0.03 | 3.2 | 3.8bc |
0.3 | 0.05 | 3.7 | 5.0c |
0.5 | 0.01 | 1.2 | 1.4a |
0.5 | 0.03 | 1.3 | 1.4a |
0.5 | 0.05 | 1.4 | 1.6a |
Tab. 2 Propagation and embryos formation of the embryonic callus from T. cyanea
2,4-D/ (mg·L-1) | KT/ (mg·L-1) | 平均每代增殖体积Volume increment/mL | 平均每个周期增殖系数Multiplication |
---|---|---|---|
0.1 | 0 | — | — |
0.3 | 0 | — | — |
0.5 | 0 | — | — |
0.1 | 0.01 | 2.9 | 3.0b |
0.1 | 0.03 | 3.1 | 3.3b |
0.1 | 0.05 | 3.1 | 3.3b |
0.3 | 0.01 | 3.2 | 3.5bc |
0.3 | 0.03 | 3.2 | 3.8bc |
0.3 | 0.05 | 3.7 | 5.0c |
0.5 | 0.01 | 1.2 | 1.4a |
0.5 | 0.03 | 1.3 | 1.4a |
0.5 | 0.05 | 1.4 | 1.6a |
6-BA/ (mg·L-1) | NAA/ (mg·L-1) | 胚块萌发率 Germination rate of embryo piece/% | 胚萌发率 Germination rate of embryos/% |
---|---|---|---|
0.1 | 0.05 | 30a | 11a |
0.5 | 0.05 | 50b | 23ab |
1.0 | 0.05 | 82c | 32b |
2.0 | 0.05 | 80c | 55c |
0.1 | 0.02 | 42a | 21a |
0.5 | 0.02 | 64b | 32b |
1.0 | 0.02 | 96c | 57c |
2.0 | 0.02 | 96c | 56c |
0.1 | 0.01 | 40a | 39b |
0.5 | 0.01 | 62b | 43b |
1.0 | 0.01 | 100c | 90c |
2.0 | 0.01 | 100c | 81c |
Tab. 3 Embryos ermination of T. cyanea
6-BA/ (mg·L-1) | NAA/ (mg·L-1) | 胚块萌发率 Germination rate of embryo piece/% | 胚萌发率 Germination rate of embryos/% |
---|---|---|---|
0.1 | 0.05 | 30a | 11a |
0.5 | 0.05 | 50b | 23ab |
1.0 | 0.05 | 82c | 32b |
2.0 | 0.05 | 80c | 55c |
0.1 | 0.02 | 42a | 21a |
0.5 | 0.02 | 64b | 32b |
1.0 | 0.02 | 96c | 57c |
2.0 | 0.02 | 96c | 56c |
0.1 | 0.01 | 40a | 39b |
0.5 | 0.01 | 62b | 43b |
1.0 | 0.01 | 100c | 90c |
2.0 | 0.01 | 100c | 81c |
基本培养基 Basal medium | IBA/ (mg·L-1) | NAA/ (mg·L-1) | 接种不定芽数 Number of explants | 生根率 Rooting rate/% | 状态 Status of seedlings |
---|---|---|---|---|---|
MS | 0.5 | 0 | 50 | 52c | 芽小,根粗壮,根毛少 |
MS | 0.5 | 0.5 | 50 | 39ab | 芽略高,根粗壮,根毛正常 |
MS | 0.5 | 1.0 | 50 | 42b | 芽较高,根正常 |
MS | 0.5 | 1.5 | 50 | 43b | 芽高,根较细弱 |
1/2 MS | 0.5 | 0 | 50 | 30a | 芽小,根粗壮,根毛少 |
1/2 MS | 0.5 | 0.5 | 50 | 41ab | 芽略高,根粗壮,根毛正常 |
1/2 MS | 0.5 | 1.0 | 50 | 47c | 芽较高,根正常 |
1/2 MS | 0.5 | 1.5 | 50 | 48c | 芽高,根细弱 |
Tab. 4 Rooting of the adventitious shoots from T. cyanea
基本培养基 Basal medium | IBA/ (mg·L-1) | NAA/ (mg·L-1) | 接种不定芽数 Number of explants | 生根率 Rooting rate/% | 状态 Status of seedlings |
---|---|---|---|---|---|
MS | 0.5 | 0 | 50 | 52c | 芽小,根粗壮,根毛少 |
MS | 0.5 | 0.5 | 50 | 39ab | 芽略高,根粗壮,根毛正常 |
MS | 0.5 | 1.0 | 50 | 42b | 芽较高,根正常 |
MS | 0.5 | 1.5 | 50 | 43b | 芽高,根较细弱 |
1/2 MS | 0.5 | 0 | 50 | 30a | 芽小,根粗壮,根毛少 |
1/2 MS | 0.5 | 0.5 | 50 | 41ab | 芽略高,根粗壮,根毛正常 |
1/2 MS | 0.5 | 1.0 | 50 | 47c | 芽较高,根正常 |
1/2 MS | 0.5 | 1.5 | 50 | 48c | 芽高,根细弱 |
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