Genetic Diversity Analysis of Thirteen Mango Germplasm Resources Based on SRAP Molecular Markers

doi:10.3969/j.issn.1000-2561.2019.01.013

Abstract

Abstract:

The genetic diversity and genetic relationship of mango germplasm resources were explored in Guizhou to provide the theoretical basis for mango germplasm identification, innovation and utilization. SRAP was used to assess the genetic diversity of 13 mango germplasm resources. 161 bands were amplified using 17 pairs of primers selected from 108 primers, of which 133 were polymorphic bands, and the polymorphic percentage of bands was 82.61%. Genetic similarity coefficients between genotypes was in the range of 0.602-0.820, with the minimum between mango Guire 10 and Panxi Red Mango, and the maximum between Guire 82 and Hongxiangya. Cluster analysis results showed that Mi-8 and Guire 82 had the closest relatives. Mi-9 and Red Mango 6 had the closest relatives. Mi-5, Chuan Mango, Guire 10 and Panxi Red Mango had the closest genetic relationship. Mi-6, Kate Mango and Jinhuang Mango had the closest genetic relationship. The test showed that SRAP technology could be used in the genetic diversity analysis of mango germplasm resources in Guizhou, and the genetic background of mango germplasm resources was complex in Guizhou.

Key words: mango, germplasm resources, SRAP, cluster analysis, Guizhou

LIU Rong,GONG Deyong,LIU Qingguo,HUANG Hai,FAN Jianxin. Genetic Diversity Analysis of Thirteen Mango Germplasm Resources Based on SRAP Molecular Markers[J]. Chinese Journal of Tropical Crops, 2019, 40(1): 87-91.

Figures/Tables 6

References 25

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编号Number	种质资源Germplasm resource	基本特征Basic characteristic
1	串芒	象牙芒22号芽变单株,引种于广西,多胚,适应性强,中熟品种
2	桂热芒10号	中大型果,多胚,抗寒性强,后期果实易感炭疽病
3	红芒6号	皮薄果软,易感炭疽病,抗寒性强
4	Mi-5	贵州野生种质资源,抗寒性强
5	台农1号	早熟品种,抗炭疽病强,耐贮运
6	Mi-6	贵州野生种质资源,抗寒性强
7	红象牙芒	引种于广西,单胚
8	攀西红芒	引种于四川,多胚,中熟品种,适应性强
9	桂热芒82号	引种于广西,单胚,晚熟品种
10	凯特芒	原产于美国,单胚
11	金煌芒	台湾自育品种,多胚,中熟品种
12	Mi-8	桂热芒82号实生单株,中大型果,抗寒性强,晚熟品种
13	Mi-9	红象牙芒实生单株,中小型果,抗寒能力强,中晚熟品种

编号Number	种质资源Germplasm resource	基本特征Basic characteristic
1	串芒	象牙芒22号芽变单株,引种于广西,多胚,适应性强,中熟品种
2	桂热芒10号	中大型果,多胚,抗寒性强,后期果实易感炭疽病
3	红芒6号	皮薄果软,易感炭疽病,抗寒性强
4	Mi-5	贵州野生种质资源,抗寒性强
5	台农1号	早熟品种,抗炭疽病强,耐贮运
6	Mi-6	贵州野生种质资源,抗寒性强
7	红象牙芒	引种于广西,单胚
8	攀西红芒	引种于四川,多胚,中熟品种,适应性强
9	桂热芒82号	引种于广西,单胚,晚熟品种
10	凯特芒	原产于美国,单胚
11	金煌芒	台湾自育品种,多胚,中熟品种
12	Mi-8	桂热芒82号实生单株,中大型果,抗寒性强,晚熟品种
13	Mi-9	红象牙芒实生单株,中小型果,抗寒能力强,中晚熟品种

正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
ME1: TGAGTCCAAACCGGATA	EM1: GACTGCGTACGAATTAAT
ME2: TGAGTCCAAACCGGAGC	EM2: GACTGCGTACGAATTTGC
ME3: TGAGTCCAAACCGGAAT	EM3: GACTGCGTACGAATTGAC
ME4: TGAGTCCAAACCGGACC	EM4: GACTGCGTACGAATTTGA
ME5: TGAGTCCAAACCGGAAG	EM5: GACTGCGTACGAATTAAC
ME6: TGAGTCCAAACCGGTAG	EM6: GACTGCGTACGAATTGCA
ME7: TGAGTCCAAACCGGTAA	EM7: GACTGCGTACGAATTATG
ME8: TGAGTCCAAACCGGTGC	EM8: GACTGCGTACGAATTAGC
ME9: TGAGTCCAAACCGGTCC	EM9: GACTGCGTACGAATTACG
	EM10: GACTGCGTACGAATTCAG
	EM11: GACTGCGTACGAATTCTG
	EM12: GACTGCGTACGAATTCCA

正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
ME1: TGAGTCCAAACCGGATA	EM1: GACTGCGTACGAATTAAT
ME2: TGAGTCCAAACCGGAGC	EM2: GACTGCGTACGAATTTGC
ME3: TGAGTCCAAACCGGAAT	EM3: GACTGCGTACGAATTGAC
ME4: TGAGTCCAAACCGGACC	EM4: GACTGCGTACGAATTTGA
ME5: TGAGTCCAAACCGGAAG	EM5: GACTGCGTACGAATTAAC
ME6: TGAGTCCAAACCGGTAG	EM6: GACTGCGTACGAATTGCA
ME7: TGAGTCCAAACCGGTAA	EM7: GACTGCGTACGAATTATG
ME8: TGAGTCCAAACCGGTGC	EM8: GACTGCGTACGAATTAGC
ME9: TGAGTCCAAACCGGTCC	EM9: GACTGCGTACGAATTACG
	EM10: GACTGCGTACGAATTCAG
	EM11: GACTGCGTACGAATTCTG
	EM12: GACTGCGTACGAATTCCA

引物组合 Primer pair	扩增总条带数 Total number of bands	多态性带数 Number of polymorphic bands	多态性比率 Rate of polymorphic bands/%
ME1-EM6	12	11	91.67
ME1-EM11	8	8	100.00
ME2-EM1	6	5	83.33
ME2-EM10	7	4	57.14
ME3-EM3	5	3	60.00
ME3-EM10	10	8	80.00
ME4-EM2	8	8	100.00
ME4-EM3	9	9	100.00
ME4-EM4	13	11	84.62
ME4-EM5	18	18	100.00
ME5-EM3	10	6	60.00
ME5-EM6	7	6	85.71
ME5-EM7	9	7	77.78
ME5-EM9	11	8	72.73
ME6-EM3	8	5	62.50
ME7-EM6	12	10	83.33
ME7-EM8	8	6	75.00