Karyotype and Cluster Analysis of Five Cassava (<i>Manihot esculenta </i>Crantz) Varieties
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 79-86.DOI: 10.3969/j.issn.1000-2561.2019.01.012

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Karyotype and Cluster Analysis of Five Cassava (Manihot esculenta Crantz) Varieties

LI Xiaoli1,2,HE Xinya1,XIAO Xinhui2,GAO Heqiong1,ZHUANG Nansheng1,WANG Ying1,*()   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China
  • Received:2018-06-04 Revised:2018-09-12 Online:2019-01-15 Published:2019-01-15
  • Contact: WANG Ying


Karyotype analysis was performed on five cassava cultivars to reveal the karyotype characteristics. The similarity and genetic relationship among the five cultivars were analyzed by cluster analysis, using young leaves as the materials and the squashing method to analyze the features of chromosomes. The results showed that the chromosome number of the five varieties was 2n=36, and the karyotype formula of SC12, SM2300-1, Guire No. 5 was 2n=36=34m+2sm, 2n=36=36m(4SAT), 2n=36=36m, respectively, and that of both Yunnan No. 8 and ZM8752 was 2n=36=34m(4SAT)+2sm. The asymmetrical karyotype coefficient was ranged from 56.58 to 58.85, and the karyotype types was 1A, 1B, 1B, 2A and 1A, respectively. The degree of symmetry was high. The clustering results showed that when the genetic distance was 0.4, the five varieties were divided into 3 categories. The first category was Yunnan No. 8, the second category included ZM8752 and SM2300-1, and the third category included SC12 and Guire No. 5. The five cultivars had certain karyotype differences, indicating that they had abundant genetic diversity.

Key words: cassava, karyotype analysis, cluster analysis