Histochemical and Molecular Characterization of Laticifer Cells in Calli Derived from Petioles of Rubber Tree
Welcome to Chinese Journal of Tropical Crops,

Chinese Journal of Tropical Crops ›› 2019, Vol. 40 ›› Issue (1): 73-78.DOI: 10.3969/j.issn.1000-2561.2019.01.011

• Biotechnology and Tissue Culture • Previous Articles     Next Articles

Histochemical and Molecular Characterization of Laticifer Cells in Calli Derived from Petioles of Rubber Tree

TAN Deguan,PENG Jing,HAN Bingying,FU Lili,SUN Xuepiao,ZHANG Jiaming()   

  1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
  • Received:2018-07-10 Revised:2018-08-13 Online:2019-01-15 Published:2019-01-15
  • Contact: ZHANG Jiaming

Abstract:

The trunk bark of rubber tree (Hevea brasiliensis) is usually used as the material for the study of laticifer differentiation. In this study, we confirmed the presence of laticifer cells in petiole-derived calli by histochemical staining, nile red fluorescence staining, immunohistochemistry and molecular biology. Laticifer cells in petiole-derived calli were distributed randomly, similar to the primary laticifers. They contained less rubber particles in the early developmental stage, and were full of rubber particles in the late developmental stage. The laticifer-specific genes including REF, SRPP, Hevein and CPT were confirmed to be expressed from the petiole-derived callus by RT-PCR, and their transcripts were the same as those reported in the latex of the trunk barks. These results indicated that the laticifer cells in the petiole-derived calli and in barks had similar functions, which would provide a basis for petiole-derived callus as a novel research model to study laticifer differentiation in the rubber tree.

Key words: rubber tree (Hevea brasiliensis), petiole-derived calli, laticifer cells, laticfer differentiation, research model