欢迎访问《热带作物学报》,

热带作物学报 ›› 2020, Vol. 41 ›› Issue (5): 1007-1012.doi: 10.3969/j.issn.1000-2561.2020.05.022

• 植物病理、作物病虫害及其防治 • 上一篇    下一篇

降香黄檀叶枯病菌的鉴定及其生物学特性

陈瑶,李航宇,周德明()   

  1. 中南林业科技大学/南方人工林病虫害防控国家林草局重点实验室/森林有害生物防控湖南省重点实验室/经济林培育与保护教育部重点实验室,湖南长沙 410000
  • 收稿日期:2019-06-27 修回日期:2019-09-15 出版日期:2020-05-25 发布日期:2020-06-15
  • 通讯作者: 周德明 E-mail:499849637@qq.com
  • 作者简介:陈 瑶(1994—),男,硕士研究生,研究方向:森林保护学。
  • 基金资助:
    “十三五”国家重点研发计划课题(2016YFD0600601-1)

Identification and Biological Characteristics of Dalbergia odorifera Leaf Blight

CHEN Yao,LI Hangyu,ZHOU Deming()   

  1. Central South University of Forestry and Technology / Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China / Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests / Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Changsha, Hunan 410000, China
  • Received:2019-06-27 Revised:2019-09-15 Online:2020-05-25 Published:2020-06-15
  • Contact: ZHOU Deming E-mail:499849637@qq.com

摘要:

近年来,降香黄檀叶枯病的危害越来越严重,为深入了解该病菌特征,本研究对该病原菌进行鉴定,以期为制定更有效的防治措施提供依据。本研究对降香黄檀叶枯病病株进行病原菌分离,并对病原菌进行了形态学观察及rDNA-ITS序列测定,确定该病原菌为天门冬拟茎点霉菌[Phomopsis asparagi (Sacc.) Bubak]。通过观察该病原菌在不同培养基、温度、pH、光照等4种环境因素中的生长情况,发现该病原菌在马铃薯果糖琼脂培养基(PFA)中菌丝生长最快;菌丝在10~30 ℃均能生长,最适宜的生长温度为25~30 ℃;最适宜的pH为6;光照对该病原菌的生长没有显著影响。

关键词: 降香黄檀, 叶枯病, 天门冬拟茎点霉, 鉴定, 生物学特性

Abstract:

In recent years, the harmfulness of Dalbergia odorifera leaf blight has become more and more serious. In order to provide a basis for the control and comprehensive treatment of this disease, the pathogenic fungus causing the disease was isolated and identified and the biological characteristics were studied. In this study, the pathogenic fungus was isolated from the pathogen of D. odorifera leaf blight, and the morphological observation of the pathogenic fungi was carried out. And the rDNA-ITS sequence was obtained by sequencing. The pathogenic fungus was identified as Phomopsis asparagi. Through experiments on the growth medium, temperature, pH value and illumination of the pathogens, the mycelial growth of the fungus in PFA medium was the fastest. The mycelium could grow at 10-30 ℃, and the optimum temperature for mycelium growth was 25-30 ℃. The optimum pH for the mycelium growth was 6. Light had no significant effect on the growth of the pathogen.

Key words: Dalbergia odorifera, leaf blight, Phomopsis asparagi, identification, biological characteristics

中图分类号: 

  • S763.15