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热带作物学报 ›› 2020, Vol. 41 ›› Issue (5): 985-993.doi: 10.3969/j.issn.1000-2561.2020.05.019

• 生物技术与组织培养 • 上一篇    下一篇

墨兰‘吴字翠’× 墨兰‘红花’杂交后代根状茎增殖及芽分化研究

谢娟,李龙,邢玥,翁青史,樊晚林,彭东辉()   

  1. 福建农林大学园林学院/国家林业草原局兰科植物保育与利用重点实验室,福建福州 350002
  • 收稿日期:2019-05-10 修回日期:2019-09-12 出版日期:2020-05-25 发布日期:2020-06-15
  • 通讯作者: 彭东辉 E-mail:fjpdh@126.com
  • 作者简介:谢 娟(1994—),女,硕士研究生,研究方向:园林植物与应用。
  • 基金资助:
    福建省种业创新与产业化工程项目(ZYCX-LY-2017005);国家重点研发计划(2019YFD10011003);福建农林大学科技创新基金(KFA17100A)

Rhizome Proliferation and Bud differentiation of Cymbiduim sinense ‘Wuzicui’ × C. sinense ‘Honghua’ Hybrid

XIE Juan,LI Long,XING Yue,WENG Qingshi,FAN Wanlin,PENG Donghui()   

  1. College of Landscape Architecture, Fujian Agriculture and Forestry University / Key Laboratory of Orchidaceous Plant Conservation and Utilization, State Forestry and Grassland Administration, Fuzhou, Fujian 350002, China
  • Received:2019-05-10 Revised:2019-09-12 Online:2020-05-25 Published:2020-06-15
  • Contact: PENG Donghui E-mail:fjpdh@126.com

摘要:

墨兰(Cymbiduim sinense)又称报岁兰,是兰科兰属植物,以其株型健壮、花期在春节深受国人喜爱。鉴于墨兰组培根状茎增殖率低、分化成芽困难,本研究以杂交墨兰根状茎为材料,通过不同浓度的生长素、分裂素以及有机添加物的组合,以期找到适宜墨兰根状茎增殖和分化的培养基配方。结果表明,最适宜杂交墨兰根状茎增殖的培养基配方为MS+ 2 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L TDZ+1.5 g/L AC+35.0 g/L Su+6.0 g/L Ag,增殖系数达5.35,生长速度为3.44。在添加不同激素的情况下,最适宜叶芽分化的培养基为MS+10.0 mg/L 6-BA+0.7 mg/L NAA+0.2 mg/L TDZ+1.5 g/L AC+35.0 g/L Su+6.0 g/L Ag,叶芽分化率为52.5%。在添加其他添加物的情况下,墨兰叶芽分化最适宜的培养基为MS+8.0 mg/L 6-BA+0.1 mg/L NAA+7.0 g/L蛋白胨+100.0 mg/L CW+1.0 g/L AC,叶芽分化率为173.30%。本研究探讨了激素和添加物对根状茎增殖分化的影响,可实现杂交品种组培苗大量繁殖。

关键词: 墨兰, 根状茎, 增殖, 分化, 诱导

Abstract:

Cymbiduim sinense is also called Bao Sui Lan (Heralding New Year Orchid), which belongs to Cymbiduim (Orchidaceae). It is loved by Chinese people for its robust plant type and flowering period during the Spring Festival. In view of the low proliferation rate and the difficulty of bud differentiation of the rhizome of C. sinense. In this study, the hybrid rhizomes of C. sinense were used as materials, through the combination of auxin, mitogen and organic additives in different concentrations, in order to find a suitable medium formula for the proliferation and differentiation of the rhizomes of hybrid C. sinense. The results showed that the most suitable medium for rhizomes proliferation was MS+ 8 mg/L 6-BA+0.1 mg/L NAA+0.8 mg/L TDZ+1.5 g/L AC+35.0 g/L Su+6.0 g/L Ag, with a proliferation coefficient 5.35 and the growth rate 3.44. The most suitable medium for leaf bud differentiation was MS+10.0 mg/L 6-BA+ 0.7 mg/L NAA+0.2 mg/L TDZ+1.5 g/L AC+ 35.0 g/L Su+6.0 g/L Ag, with a leaf bud differentiation rate 52.5%. The most suitable medium for leaf bud differentiation was MS+8.0 mg/L 6-BA+0.1 mg/L NAA+7.0 g/L peptone+100.0 mg/L CW+1.0 g/L AC, with a leaf bud differentiation rate 173.30%. This study explored the effects of hormones and additives on the proliferation and differentiation of the rhizomes, which could achieve large-scale reproduction of the tissue culture seedlings.

Key words: Cymbiduim sinense, rhizome, proliferation, differentiation, induction

中图分类号: 

  • S682.31