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热带作物学报 ›› 2020, Vol. 41 ›› Issue (5): 947-954.doi: 10.3969/j.issn.1000-2561.2020.05.014

• 生物技术与组织培养 • 上一篇    下一篇

可可FAT基因家族进化及表达模式分析

李付鹏1,邓云梅1,2,伍宝朵1,秦晓威1,闫林1,赖剑雄1,*()   

  1. 1. 中国热带农业科学院香料饮料研究所/农业农村部香辛饮料作物遗传资源利用重点实验室/海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
    2. 云南农业大学热带作物学院,云南普洱 665099
  • 收稿日期:2019-05-12 修回日期:2019-09-02 出版日期:2020-05-25 发布日期:2020-06-15
  • 通讯作者: 赖剑雄 E-mail:ljx619@163.com
  • 作者简介:李付鹏(1984—),男,博士,副研究员,研究方向:作物种质资源与遗传育种。
  • 基金资助:
    国家自然科学基金面上项目(31670684);中国热带农业科学院基本科研业务费专项(1630142019003)

Phylogeny and Expression Profile of Fatty Acyl-ACP Thioesterase (FAT) Gene Family in Cacao (Theobroma cacao L.)

LI Fupeng1,DENG Yunmei1,2,WU Baoduo1,QIN Xiaowei1,YAN Lin1,LAI Jianxiong1,*()   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs / Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
    2. College of Tropical Crop Science, Yunnan Agricultural University, Pu'er, Yunnan 665099, China
  • Received:2019-05-12 Revised:2019-09-02 Online:2020-05-25 Published:2020-06-15
  • Contact: LAI Jianxiong E-mail:ljx619@163.com

摘要:

在植物中,酰基-ACP硫酯酶(fatty acyl-ACP thioesterase, FAT)是调控脂肪酸合成的关键酶。为解析可可FAT基因家族成员的特点与功能,本研究从可可基因组中筛选鉴定出FAT基因家族的6个成员,分别命名为TcFATATcFATB1TcFATB2TcFATB3TcFATB4TcFATB5。6个基因外显子数目为6~7个,编码区(CDS)长度介于1128~1263 bp,预测蛋白分子量介于42.72~46.47 kDa,等电点介于6.57~9.10。进化分析结果表明可可FAT基因家族分成FATA与FATB亚群,FATA亚群包含1个可可TcFATA成员,FATB亚群包含5个可可TcFATBs成员。不同可可种子发育时期表达分析结果表明:TcFATATcFATB1伴随果实发育成熟,表达量呈下降趋势,TcFATATcFATB1在不同种质中表达量与油酸(C18:1)和棕榈酸(C16:0)比例呈正相关,表明其与脂肪酸组分比例调控有紧密关联。

关键词: 可可, 酰基载体蛋白硫酯酶(FAT), 系统进化, 表达模式

Abstract:

Fatty acyl-ACP thioesterase (FAT) is the key enzyme regulating the synthesis of plant lipid. In order to better understand the characteristics of FAT gene family in Theobroma cacao, six novel FAT genes were identified from economically important cacao trees, designated as TcFATA, TcFATB1, TcFATB2, TcFATB3, TcFATB4, TcFATB5, respectively. Sequences analyses revealed that CDS of TcFATs was 1128-1263 bp, containing 6-7 exons. The molecular weight of the six predicted protein was 42.72-46.47 kDa, and pI of the proteins was 6.57-9.10. FAT family could be divided into FATA (one TcFATA) and FATB (five TcFATBs) subfamily. The expression patterns of the genes were investigated via real-time PCR in various developmental phases. The transcription level of TcFATA and TcFATB1 was continuously decreased along with pod development. Furthermore, the expression level of TcFATA and TcFATB1 was positively associated with the ratio of oleic acid (C18:1) and palmitic acid (C16:0) respectively. The results indicate that TcFATA and TcFATB1 are significantly related with the regulation of fatty acid component.

Key words: Theobroma cacao, fatty acyl-ACP thioesterase (FAT), phylogenetic, expression profile

中图分类号: 

  • S571.3